High density micromass cultures of a human chondrocyte cell line: a reliable assay system to reveal the modulatory functions of pharmacological agents

Research output: Contribution to journalArticle

Standard

High density micromass cultures of a human chondrocyte cell line : a reliable assay system to reveal the modulatory functions of pharmacological agents. / Greco, K V; Iqbal, A J; Rattazzi, L; Nalesso, G; Moradi-Bidhendi, N; Moore, A R; Goldring, M B; Dell'Accio, F; Perretti, M.

In: Biochemical Pharmacology, Vol. 82, No. 12, 15.12.2011, p. 1919-29.

Research output: Contribution to journalArticle

Harvard

Greco, KV, Iqbal, AJ, Rattazzi, L, Nalesso, G, Moradi-Bidhendi, N, Moore, AR, Goldring, MB, Dell'Accio, F & Perretti, M 2011, 'High density micromass cultures of a human chondrocyte cell line: a reliable assay system to reveal the modulatory functions of pharmacological agents', Biochemical Pharmacology, vol. 82, no. 12, pp. 1919-29. https://doi.org/10.1016/j.bcp.2011.09.009

APA

Greco, K. V., Iqbal, A. J., Rattazzi, L., Nalesso, G., Moradi-Bidhendi, N., Moore, A. R., Goldring, M. B., Dell'Accio, F., & Perretti, M. (2011). High density micromass cultures of a human chondrocyte cell line: a reliable assay system to reveal the modulatory functions of pharmacological agents. Biochemical Pharmacology, 82(12), 1919-29. https://doi.org/10.1016/j.bcp.2011.09.009

Vancouver

Author

Greco, K V ; Iqbal, A J ; Rattazzi, L ; Nalesso, G ; Moradi-Bidhendi, N ; Moore, A R ; Goldring, M B ; Dell'Accio, F ; Perretti, M. / High density micromass cultures of a human chondrocyte cell line : a reliable assay system to reveal the modulatory functions of pharmacological agents. In: Biochemical Pharmacology. 2011 ; Vol. 82, No. 12. pp. 1919-29.

Bibtex

@article{68602559c6f84aa5bd6dd0ad314ccdff,
title = "High density micromass cultures of a human chondrocyte cell line: a reliable assay system to reveal the modulatory functions of pharmacological agents",
abstract = "Osteoarthritis is a highly prevalent and disabling disease for which we do not have a cure. The identification of suitable molecular targets is hindered by the lack of standardized, reproducible and convenient screening assays. Following extensive comparisons of a number of chondrocytic cell lines, culture conditions, and readouts, we have optimized an assay utilizing C-28/I2, a chondrocytic cell line cultured in high-density micromasses. Utilizing molecules with known effects on cartilage (e.g. IL-1β, TGFβ1, BMP-2), we have exploited this improved protocol to (i) evoke responses characteristic of primary chondrocytes; (ii) assess the pharmacodynamics of gene over-expression using non-viral expression vectors; (iii) establish the response profiles of known pharmacological treatments; and (iv) investigate their mechanisms of action. These data indicate that we have established a medium-throughput methodology for studying chondrocyte-specific cellular and molecular responses (from gene expression to rapid quantitative measurement of sulfated glycosaminoglycans by Alcian blue staining) that may enable the discovery of novel therapeutics for pharmacological modulation of chondrocyte activation in osteoarthritis.",
keywords = "Alcian Blue, Anti-Inflammatory Agents, Cartilage, Articular, Cell Line, Cell Proliferation, Chondrocytes, Gene Expression Regulation, Humans, Interleukin-1beta, Naproxen, Prednisolone, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't",
author = "Greco, {K V} and Iqbal, {A J} and L Rattazzi and G Nalesso and N Moradi-Bidhendi and Moore, {A R} and Goldring, {M B} and F Dell'Accio and M Perretti",
note = "Copyright {\^A}{\textcopyright} 2011 Elsevier Inc. All rights reserved.",
year = "2011",
month = dec
day = "15",
doi = "10.1016/j.bcp.2011.09.009",
language = "English",
volume = "82",
pages = "1919--29",
journal = "Biochemical Pharmacology",
issn = "0006-2952",
publisher = "Elsevier",
number = "12",

}

RIS

TY - JOUR

T1 - High density micromass cultures of a human chondrocyte cell line

T2 - a reliable assay system to reveal the modulatory functions of pharmacological agents

AU - Greco, K V

AU - Iqbal, A J

AU - Rattazzi, L

AU - Nalesso, G

AU - Moradi-Bidhendi, N

AU - Moore, A R

AU - Goldring, M B

AU - Dell'Accio, F

AU - Perretti, M

N1 - Copyright © 2011 Elsevier Inc. All rights reserved.

PY - 2011/12/15

Y1 - 2011/12/15

N2 - Osteoarthritis is a highly prevalent and disabling disease for which we do not have a cure. The identification of suitable molecular targets is hindered by the lack of standardized, reproducible and convenient screening assays. Following extensive comparisons of a number of chondrocytic cell lines, culture conditions, and readouts, we have optimized an assay utilizing C-28/I2, a chondrocytic cell line cultured in high-density micromasses. Utilizing molecules with known effects on cartilage (e.g. IL-1β, TGFβ1, BMP-2), we have exploited this improved protocol to (i) evoke responses characteristic of primary chondrocytes; (ii) assess the pharmacodynamics of gene over-expression using non-viral expression vectors; (iii) establish the response profiles of known pharmacological treatments; and (iv) investigate their mechanisms of action. These data indicate that we have established a medium-throughput methodology for studying chondrocyte-specific cellular and molecular responses (from gene expression to rapid quantitative measurement of sulfated glycosaminoglycans by Alcian blue staining) that may enable the discovery of novel therapeutics for pharmacological modulation of chondrocyte activation in osteoarthritis.

AB - Osteoarthritis is a highly prevalent and disabling disease for which we do not have a cure. The identification of suitable molecular targets is hindered by the lack of standardized, reproducible and convenient screening assays. Following extensive comparisons of a number of chondrocytic cell lines, culture conditions, and readouts, we have optimized an assay utilizing C-28/I2, a chondrocytic cell line cultured in high-density micromasses. Utilizing molecules with known effects on cartilage (e.g. IL-1β, TGFβ1, BMP-2), we have exploited this improved protocol to (i) evoke responses characteristic of primary chondrocytes; (ii) assess the pharmacodynamics of gene over-expression using non-viral expression vectors; (iii) establish the response profiles of known pharmacological treatments; and (iv) investigate their mechanisms of action. These data indicate that we have established a medium-throughput methodology for studying chondrocyte-specific cellular and molecular responses (from gene expression to rapid quantitative measurement of sulfated glycosaminoglycans by Alcian blue staining) that may enable the discovery of novel therapeutics for pharmacological modulation of chondrocyte activation in osteoarthritis.

KW - Alcian Blue

KW - Anti-Inflammatory Agents

KW - Cartilage, Articular

KW - Cell Line

KW - Cell Proliferation

KW - Chondrocytes

KW - Gene Expression Regulation

KW - Humans

KW - Interleukin-1beta

KW - Naproxen

KW - Prednisolone

KW - Journal Article

KW - Research Support, N.I.H., Extramural

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.bcp.2011.09.009

DO - 10.1016/j.bcp.2011.09.009

M3 - Article

C2 - 21946086

VL - 82

SP - 1919

EP - 1929

JO - Biochemical Pharmacology

JF - Biochemical Pharmacology

SN - 0006-2952

IS - 12

ER -