Heterogeneity of lymphoid tissue inducer cell populations present in embryonic and adult mouse lymphoid tissues

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@article{2e9112426b1d466c9d478e60e0ad996a,
title = "Heterogeneity of lymphoid tissue inducer cell populations present in embryonic and adult mouse lymphoid tissues",
abstract = "Lymphoid tissue inducer (LTi) cells have a well established role in secondary lymphoid tissue development. Here, we report on the heterogeneity of LTi cells based on their CD4 and chemokine receptor expression. The CD4(-) LTi-cell population has a similar phenotype to the CD4(+) population, with similar chemokine-receptor-expressing subsets. In both embryonic and adult spleen the CD4(-) LTi-cell population is comparable as a proportion of total splenocytes to its CD4(+) counterpart. In contrast, different proportions of CD4(+) and CD4(-) LTi cells are found in different lymph nodes. Both CD4(+) and CD4(-) LTi cells share the anatomical location and are associated with vascular cell adhesion molecule-1-positive stromal cells in spleen and lymph nodes. The numbers of both CD4(+) and CD4(-) LTi cells in adult spleen are augmented in the presence of B cells. With the exception of CD4, there is a strong correlation coefficient (0.89) for gene expression between the two populations. Polymerase chain reaction analysis of individual CD4(+) and CD4(-) LTi cells shows that a similar proportion in embryonic and adult spleen co-expressed both CXCR5 and CCR7 or CXCR5 alone: 84.6% for adult CD4(+) and 87.6% for adult CD4(-); 95.3% for embryonic CD4(+) and 91.5% for embryonic CD4(-). Consistently fewer CCR7 single-positive cells were found in the CD4(+) and CD4(-) fractions in the embryo.",
keywords = "tumour necrosis factor and tumour necrosis factor receptor family members, CCR7, CXCR5, lymphoid tissue inducer cell",
author = "MY Kim and S Rossi and David Withers and F McConnell and Kai-Michael Toellner and Fabrina Gaspal and Eric Jenkinson and Graham Anderson and Peter Lane",
year = "2008",
month = jun,
day = "1",
doi = "10.1111/j.1365-2567.2007.02750.x",
language = "English",
volume = "124",
pages = "166--74",
journal = "Immunology",
issn = "0019-2805",
publisher = "Wiley",
number = "2",

}

RIS

TY - JOUR

T1 - Heterogeneity of lymphoid tissue inducer cell populations present in embryonic and adult mouse lymphoid tissues

AU - Kim, MY

AU - Rossi, S

AU - Withers, David

AU - McConnell, F

AU - Toellner, Kai-Michael

AU - Gaspal, Fabrina

AU - Jenkinson, Eric

AU - Anderson, Graham

AU - Lane, Peter

PY - 2008/6/1

Y1 - 2008/6/1

N2 - Lymphoid tissue inducer (LTi) cells have a well established role in secondary lymphoid tissue development. Here, we report on the heterogeneity of LTi cells based on their CD4 and chemokine receptor expression. The CD4(-) LTi-cell population has a similar phenotype to the CD4(+) population, with similar chemokine-receptor-expressing subsets. In both embryonic and adult spleen the CD4(-) LTi-cell population is comparable as a proportion of total splenocytes to its CD4(+) counterpart. In contrast, different proportions of CD4(+) and CD4(-) LTi cells are found in different lymph nodes. Both CD4(+) and CD4(-) LTi cells share the anatomical location and are associated with vascular cell adhesion molecule-1-positive stromal cells in spleen and lymph nodes. The numbers of both CD4(+) and CD4(-) LTi cells in adult spleen are augmented in the presence of B cells. With the exception of CD4, there is a strong correlation coefficient (0.89) for gene expression between the two populations. Polymerase chain reaction analysis of individual CD4(+) and CD4(-) LTi cells shows that a similar proportion in embryonic and adult spleen co-expressed both CXCR5 and CCR7 or CXCR5 alone: 84.6% for adult CD4(+) and 87.6% for adult CD4(-); 95.3% for embryonic CD4(+) and 91.5% for embryonic CD4(-). Consistently fewer CCR7 single-positive cells were found in the CD4(+) and CD4(-) fractions in the embryo.

AB - Lymphoid tissue inducer (LTi) cells have a well established role in secondary lymphoid tissue development. Here, we report on the heterogeneity of LTi cells based on their CD4 and chemokine receptor expression. The CD4(-) LTi-cell population has a similar phenotype to the CD4(+) population, with similar chemokine-receptor-expressing subsets. In both embryonic and adult spleen the CD4(-) LTi-cell population is comparable as a proportion of total splenocytes to its CD4(+) counterpart. In contrast, different proportions of CD4(+) and CD4(-) LTi cells are found in different lymph nodes. Both CD4(+) and CD4(-) LTi cells share the anatomical location and are associated with vascular cell adhesion molecule-1-positive stromal cells in spleen and lymph nodes. The numbers of both CD4(+) and CD4(-) LTi cells in adult spleen are augmented in the presence of B cells. With the exception of CD4, there is a strong correlation coefficient (0.89) for gene expression between the two populations. Polymerase chain reaction analysis of individual CD4(+) and CD4(-) LTi cells shows that a similar proportion in embryonic and adult spleen co-expressed both CXCR5 and CCR7 or CXCR5 alone: 84.6% for adult CD4(+) and 87.6% for adult CD4(-); 95.3% for embryonic CD4(+) and 91.5% for embryonic CD4(-). Consistently fewer CCR7 single-positive cells were found in the CD4(+) and CD4(-) fractions in the embryo.

KW - tumour necrosis factor and tumour necrosis factor receptor family members

KW - CCR7

KW - CXCR5

KW - lymphoid tissue inducer cell

U2 - 10.1111/j.1365-2567.2007.02750.x

DO - 10.1111/j.1365-2567.2007.02750.x

M3 - Article

C2 - 18205791

VL - 124

SP - 166

EP - 174

JO - Immunology

JF - Immunology

SN - 0019-2805

IS - 2

ER -