Hepatitis C virus glycoproteins mediate pH-dependent cell entry of pseudotyped retroviral particles

Research output: Contribution to journalArticle

Authors

  • M Hsu
  • J Zhang
  • M Flint
  • C Logvinoff
  • C Cheng-Mayrer
  • CM Rice

Colleges, School and Institutes

Abstract

HIV pseudotypes bearing native hepatitis C virus (HCV) glycoproteins (strain H and Con1) are infectious for the human hepatoma cell lines Huh-7 and PLC/PR5. Infectivity depends on coexpression of both E1 and E2 glycoproteins, is pH-dependent, and can be neutralized by mAbs mapping to amino acids 412-447 within E2. Cell-surface expression of one or all of the candidate receptor molecules (CD81, low-density lipoprotein receptor, scavenger receptor class B type 1, and dendritic cell-specific intercellular adhesion molecule 3 grabbing nonintegrin) failed to confer permissivity to HIV-HCV pseudotype infection. However, HIV-HCV pseudotype infectivity was inhibited by a recombinant soluble form of CD81 and a mAb specific for CD81, suggesting that CD81 may be a component of a receptor complex.

Details

Original languageEnglish
Pages (from-to)7271-7276
Number of pages6
JournalNational Academy of Sciences. Proceedings
Volume100
Publication statusPublished - 2 Jun 2003