Hepatic transcriptomic and metabolomic responses in the Stickleback (Gasterosteus aculeatus) exposed to ethinyl-estradiol☆

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Hepatic transcriptomic and metabolomic responses in the Stickleback (Gasterosteus aculeatus) exposed to ethinyl-estradiol☆. / Katsiadaki, I; Williams, Timothy; Ball, J; Bean, TP; Sanders, MB; Wu, H; Santos, EM; Brown, Margaret; Baker, P; Ortega, F; Falciani, Francesco; Craft, JA; Tyler, CR; Viant, Mark; Chipman, James.

In: Aquatic Toxicology, Vol. 97, No. 3, 05.05.2010, p. 174-187.

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Katsiadaki, I ; Williams, Timothy ; Ball, J ; Bean, TP ; Sanders, MB ; Wu, H ; Santos, EM ; Brown, Margaret ; Baker, P ; Ortega, F ; Falciani, Francesco ; Craft, JA ; Tyler, CR ; Viant, Mark ; Chipman, James. / Hepatic transcriptomic and metabolomic responses in the Stickleback (Gasterosteus aculeatus) exposed to ethinyl-estradiol☆. In: Aquatic Toxicology. 2010 ; Vol. 97, No. 3. pp. 174-187.

Bibtex

@article{7c7b65a496494536a9e86da63af77fbb,
title = "Hepatic transcriptomic and metabolomic responses in the Stickleback (Gasterosteus aculeatus) exposed to ethinyl-estradiol☆",
abstract = "An established three-spined stickleback (Gasterosteus aculeatus) cDNA array was expanded to 14,496 probes with the addition of hepatic clones derived from subtractive and normalized libraries from control males and males exposed to model toxicants. Microarrays and one-dimensional H-1 nuclear magnetic resonance (NMR) spectroscopy, together with individual protein and gene biomarkers were employed to investigate the hepatic responses of the stickleback to ethinyl-estradiol (EE2) exposure. Male fish were exposed via the water to EE2, including environmentally relevant concentrations (0.1-100 ng/l) for 4 days, and hepatic transcript and metabolite profiles, kidney spiggin protein and serum vitellogenin concentrations were determined in comparison to controls. EE2 exposure did not significantly affect spiggin concentration but significantly induced serum vitellogenin protein at the threshold concentration of 32 ng/I. H-1 NMR coupled with robust univariate testing revealed only limited changes, but these did support the predicted modulation of the amino acid profile by transcriptomics. Transcriptional induction was found for hepatic vitellogenins and choriogenins as expected, together with a range of other EE2-responsive genes. Choriogenins showed the more sensitive responses with statistically significant induction at 10 ng/I. Real-time polymerase chain reaction (PCR) confirmed transcriptional induction of these genes. Phosvitinless vitellogenin C transcripts were highly expressed and represent a major form of the egg yolk precursors, and this is in contrast to other fish species where it is a minor component of vitellogenic transcripts. Differences in inducibility between the vitellogenins and choriogenins appear to be in accordance with the sequential formation of chorion and yolk during oogenesis in fish. (C) 2009 Elsevier B.V. All rights reserved.",
keywords = "Choriogenins, Ecotoxicogenomics, Estrogen, Endocrine disruption, Fish, Vitellogenins",
author = "I Katsiadaki and Timothy Williams and J Ball and TP Bean and MB Sanders and H Wu and EM Santos and Margaret Brown and P Baker and F Ortega and Francesco Falciani and JA Craft and CR Tyler and Mark Viant and James Chipman",
year = "2010",
month = may,
day = "5",
doi = "10.1016/j.aquatox.2009.07.005",
language = "English",
volume = "97",
pages = "174--187",
journal = "Aquatic Toxicology",
issn = "0166-445X",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - Hepatic transcriptomic and metabolomic responses in the Stickleback (Gasterosteus aculeatus) exposed to ethinyl-estradiol☆

AU - Katsiadaki, I

AU - Williams, Timothy

AU - Ball, J

AU - Bean, TP

AU - Sanders, MB

AU - Wu, H

AU - Santos, EM

AU - Brown, Margaret

AU - Baker, P

AU - Ortega, F

AU - Falciani, Francesco

AU - Craft, JA

AU - Tyler, CR

AU - Viant, Mark

AU - Chipman, James

PY - 2010/5/5

Y1 - 2010/5/5

N2 - An established three-spined stickleback (Gasterosteus aculeatus) cDNA array was expanded to 14,496 probes with the addition of hepatic clones derived from subtractive and normalized libraries from control males and males exposed to model toxicants. Microarrays and one-dimensional H-1 nuclear magnetic resonance (NMR) spectroscopy, together with individual protein and gene biomarkers were employed to investigate the hepatic responses of the stickleback to ethinyl-estradiol (EE2) exposure. Male fish were exposed via the water to EE2, including environmentally relevant concentrations (0.1-100 ng/l) for 4 days, and hepatic transcript and metabolite profiles, kidney spiggin protein and serum vitellogenin concentrations were determined in comparison to controls. EE2 exposure did not significantly affect spiggin concentration but significantly induced serum vitellogenin protein at the threshold concentration of 32 ng/I. H-1 NMR coupled with robust univariate testing revealed only limited changes, but these did support the predicted modulation of the amino acid profile by transcriptomics. Transcriptional induction was found for hepatic vitellogenins and choriogenins as expected, together with a range of other EE2-responsive genes. Choriogenins showed the more sensitive responses with statistically significant induction at 10 ng/I. Real-time polymerase chain reaction (PCR) confirmed transcriptional induction of these genes. Phosvitinless vitellogenin C transcripts were highly expressed and represent a major form of the egg yolk precursors, and this is in contrast to other fish species where it is a minor component of vitellogenic transcripts. Differences in inducibility between the vitellogenins and choriogenins appear to be in accordance with the sequential formation of chorion and yolk during oogenesis in fish. (C) 2009 Elsevier B.V. All rights reserved.

AB - An established three-spined stickleback (Gasterosteus aculeatus) cDNA array was expanded to 14,496 probes with the addition of hepatic clones derived from subtractive and normalized libraries from control males and males exposed to model toxicants. Microarrays and one-dimensional H-1 nuclear magnetic resonance (NMR) spectroscopy, together with individual protein and gene biomarkers were employed to investigate the hepatic responses of the stickleback to ethinyl-estradiol (EE2) exposure. Male fish were exposed via the water to EE2, including environmentally relevant concentrations (0.1-100 ng/l) for 4 days, and hepatic transcript and metabolite profiles, kidney spiggin protein and serum vitellogenin concentrations were determined in comparison to controls. EE2 exposure did not significantly affect spiggin concentration but significantly induced serum vitellogenin protein at the threshold concentration of 32 ng/I. H-1 NMR coupled with robust univariate testing revealed only limited changes, but these did support the predicted modulation of the amino acid profile by transcriptomics. Transcriptional induction was found for hepatic vitellogenins and choriogenins as expected, together with a range of other EE2-responsive genes. Choriogenins showed the more sensitive responses with statistically significant induction at 10 ng/I. Real-time polymerase chain reaction (PCR) confirmed transcriptional induction of these genes. Phosvitinless vitellogenin C transcripts were highly expressed and represent a major form of the egg yolk precursors, and this is in contrast to other fish species where it is a minor component of vitellogenic transcripts. Differences in inducibility between the vitellogenins and choriogenins appear to be in accordance with the sequential formation of chorion and yolk during oogenesis in fish. (C) 2009 Elsevier B.V. All rights reserved.

KW - Choriogenins

KW - Ecotoxicogenomics

KW - Estrogen

KW - Endocrine disruption

KW - Fish

KW - Vitellogenins

U2 - 10.1016/j.aquatox.2009.07.005

DO - 10.1016/j.aquatox.2009.07.005

M3 - Article

C2 - 19665239

VL - 97

SP - 174

EP - 187

JO - Aquatic Toxicology

JF - Aquatic Toxicology

SN - 0166-445X

IS - 3

ER -