Hepatic transcriptional responses to copper in the three-spined stickleback are affected by their pollution exposure history

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Hepatic transcriptional responses to copper in the three-spined stickleback are affected by their pollution exposure history. / Uren Webster, Tamsyn M.; Williams, Timothy D.; Katsiadaki, Ioanna; Lange, Anke; Lewis, Ceri; Shears, Janice A.; Tyler, Charles R.; Santos, Eduarda M.

In: Aquatic Toxicology, Vol. 184, 01.03.2017, p. 26-36.

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Uren Webster, Tamsyn M. ; Williams, Timothy D. ; Katsiadaki, Ioanna ; Lange, Anke ; Lewis, Ceri ; Shears, Janice A. ; Tyler, Charles R. ; Santos, Eduarda M. / Hepatic transcriptional responses to copper in the three-spined stickleback are affected by their pollution exposure history. In: Aquatic Toxicology. 2017 ; Vol. 184. pp. 26-36.

Bibtex

@article{ec7fba8fc3e8414ea6a723604d40b750,
title = "Hepatic transcriptional responses to copper in the three-spined stickleback are affected by their pollution exposure history",
abstract = "Some fish populations inhabiting contaminated environments show evidence of increased chemical tolerance, however the mechanisms contributing to this tolerance, and whether this is heritable, are poorly understood. We investigated the responses of two populations of wild three-spined stickleback (Gasterosteus aculeatus) with different histories of contaminant exposure to an oestrogen and copper, two widespread aquatic pollutants. Male stickleback originating from two sites, the River Aire, with a history of complex pollution discharges, and Siblyback Lake, with a history of metal contamination, were depurated and then exposed to copper (46 μg/L) and the synthetic oestrogen ethinyloestradiol (22 ng/L). The hepatic transcriptomic response was compared between the two populations and to a reference population with no known history of exposure (Houghton Springs, Dorset). Gene responses included those typical for both copper and oestrogen, with no discernable difference in response to oestrogen between populations. There was, however, some difference in the magnitude of response to copper between populations. Siblyback fish showed an elevated baseline transcription of genes encoding metallothioneins and a lower level of metallothionein induction following copper exposure, compared to those from the River Aire. Similarly, a further experiment with an F1 generation of Siblyback fish bred in the laboratory found evidence for elevated transcription of genes encoding metallothioneins in unexposed fish, together with an altered transcriptional response to 125 μg/L copper, compared with F1 fish originating from the clean reference population exposed to the same copper concentration. These data suggest that the stickleback from Siblyback Lake have a differential response to copper, which is inherited by the F1 generation in laboratory conditions, and for which the underlying mechanism may include an elevation of baseline transcription of genes encoding metallothioneins. The genetic and/or epigenetic mechanisms contributing to this inherited alteration of metallothionein transcription have yet to be established.",
keywords = "Metals , Pollution , Adaptation , Freshwater , Teleost",
author = "{Uren Webster}, {Tamsyn M.} and Williams, {Timothy D.} and Ioanna Katsiadaki and Anke Lange and Ceri Lewis and Shears, {Janice A.} and Tyler, {Charles R.} and Santos, {Eduarda M.}",
year = "2017",
month = mar,
day = "1",
doi = "10.1016/j.aquatox.2016.12.023",
language = "English",
volume = "184",
pages = "26--36",
journal = "Aquatic Toxicology",
issn = "0166-445X",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Hepatic transcriptional responses to copper in the three-spined stickleback are affected by their pollution exposure history

AU - Uren Webster, Tamsyn M.

AU - Williams, Timothy D.

AU - Katsiadaki, Ioanna

AU - Lange, Anke

AU - Lewis, Ceri

AU - Shears, Janice A.

AU - Tyler, Charles R.

AU - Santos, Eduarda M.

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Some fish populations inhabiting contaminated environments show evidence of increased chemical tolerance, however the mechanisms contributing to this tolerance, and whether this is heritable, are poorly understood. We investigated the responses of two populations of wild three-spined stickleback (Gasterosteus aculeatus) with different histories of contaminant exposure to an oestrogen and copper, two widespread aquatic pollutants. Male stickleback originating from two sites, the River Aire, with a history of complex pollution discharges, and Siblyback Lake, with a history of metal contamination, were depurated and then exposed to copper (46 μg/L) and the synthetic oestrogen ethinyloestradiol (22 ng/L). The hepatic transcriptomic response was compared between the two populations and to a reference population with no known history of exposure (Houghton Springs, Dorset). Gene responses included those typical for both copper and oestrogen, with no discernable difference in response to oestrogen between populations. There was, however, some difference in the magnitude of response to copper between populations. Siblyback fish showed an elevated baseline transcription of genes encoding metallothioneins and a lower level of metallothionein induction following copper exposure, compared to those from the River Aire. Similarly, a further experiment with an F1 generation of Siblyback fish bred in the laboratory found evidence for elevated transcription of genes encoding metallothioneins in unexposed fish, together with an altered transcriptional response to 125 μg/L copper, compared with F1 fish originating from the clean reference population exposed to the same copper concentration. These data suggest that the stickleback from Siblyback Lake have a differential response to copper, which is inherited by the F1 generation in laboratory conditions, and for which the underlying mechanism may include an elevation of baseline transcription of genes encoding metallothioneins. The genetic and/or epigenetic mechanisms contributing to this inherited alteration of metallothionein transcription have yet to be established.

AB - Some fish populations inhabiting contaminated environments show evidence of increased chemical tolerance, however the mechanisms contributing to this tolerance, and whether this is heritable, are poorly understood. We investigated the responses of two populations of wild three-spined stickleback (Gasterosteus aculeatus) with different histories of contaminant exposure to an oestrogen and copper, two widespread aquatic pollutants. Male stickleback originating from two sites, the River Aire, with a history of complex pollution discharges, and Siblyback Lake, with a history of metal contamination, were depurated and then exposed to copper (46 μg/L) and the synthetic oestrogen ethinyloestradiol (22 ng/L). The hepatic transcriptomic response was compared between the two populations and to a reference population with no known history of exposure (Houghton Springs, Dorset). Gene responses included those typical for both copper and oestrogen, with no discernable difference in response to oestrogen between populations. There was, however, some difference in the magnitude of response to copper between populations. Siblyback fish showed an elevated baseline transcription of genes encoding metallothioneins and a lower level of metallothionein induction following copper exposure, compared to those from the River Aire. Similarly, a further experiment with an F1 generation of Siblyback fish bred in the laboratory found evidence for elevated transcription of genes encoding metallothioneins in unexposed fish, together with an altered transcriptional response to 125 μg/L copper, compared with F1 fish originating from the clean reference population exposed to the same copper concentration. These data suggest that the stickleback from Siblyback Lake have a differential response to copper, which is inherited by the F1 generation in laboratory conditions, and for which the underlying mechanism may include an elevation of baseline transcription of genes encoding metallothioneins. The genetic and/or epigenetic mechanisms contributing to this inherited alteration of metallothionein transcription have yet to be established.

KW - Metals

KW - Pollution

KW - Adaptation

KW - Freshwater

KW - Teleost

U2 - 10.1016/j.aquatox.2016.12.023

DO - 10.1016/j.aquatox.2016.12.023

M3 - Article

VL - 184

SP - 26

EP - 36

JO - Aquatic Toxicology

JF - Aquatic Toxicology

SN - 0166-445X

ER -