Germ cell differentiation requires Tdrd7-dependent chromatin and transcriptome reprogramming marked by germ plasm relocalization

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Germ cell differentiation requires Tdrd7-dependent chromatin and transcriptome reprogramming marked by germ plasm relocalization. / D'Orazio, Fabio M; Balwierz, Piotr J; González, Ada Jimenez; Guo, Yixuan; Hernández-Rodríguez, Benjamín; Wheatley, Lucy; Jasiulewicz, Aleksandra; Hadzhiev, Yavor; Vaquerizas, Juan M; Cairns, Bradley; Lenhard, Boris; Müller, Ferenc.

In: Developmental Cell, Vol. 56, No. 5, 08.03.2021, p. 641-656.e5.

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@article{9dab9a7f215c437f90f8fd4170f7ba09,
title = "Germ cell differentiation requires Tdrd7-dependent chromatin and transcriptome reprogramming marked by germ plasm relocalization",
abstract = "In many animal models, primordial germ cell (PGC) development depends on maternally deposited germ plasm, which prevents somatic cell fate. Here, we show that PGCs respond to regulatory information from the germ plasm in two distinct phases using two distinct mechanisms in zebrafish. We demonstrate that PGCs commence zygotic genome activation together with the somatic blastocysts with no demonstrable differences in transcriptional and chromatin opening. Unexpectedly, both PGC and somatic blastocysts activate germ-cell-specific genes, which are only stabilized in PGCs by cytoplasmic germ plasm determinants. Disaggregated perinuclear relocalization of germ plasm during PGC migration is regulated by the germ plasm determinant Tdrd7 and is coupled to dramatic divergence between PGC and somatic transcriptomes. This transcriptional divergence relies on PGC-specific cis-regulatory elements characterized by promoter-proximal distribution. We show that Tdrd7-dependent reconfiguration of chromatin accessibility is required for elaboration of PGC fate but not for PGC migration.",
author = "D'Orazio, {Fabio M} and Balwierz, {Piotr J} and Gonz{\'a}lez, {Ada Jimenez} and Yixuan Guo and Benjam{\'i}n Hern{\'a}ndez-Rodr{\'i}guez and Lucy Wheatley and Aleksandra Jasiulewicz and Yavor Hadzhiev and Vaquerizas, {Juan M} and Bradley Cairns and Boris Lenhard and Ferenc M{\"u}ller",
note = "Copyright {\textcopyright} 2021 The Authors. Published by Elsevier Inc. All rights reserved.",
year = "2021",
month = mar,
day = "8",
doi = "10.1016/j.devcel.2021.02.007",
language = "English",
volume = "56",
pages = "641--656.e5",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Elsevier",
number = "5",

}

RIS

TY - JOUR

T1 - Germ cell differentiation requires Tdrd7-dependent chromatin and transcriptome reprogramming marked by germ plasm relocalization

AU - D'Orazio, Fabio M

AU - Balwierz, Piotr J

AU - González, Ada Jimenez

AU - Guo, Yixuan

AU - Hernández-Rodríguez, Benjamín

AU - Wheatley, Lucy

AU - Jasiulewicz, Aleksandra

AU - Hadzhiev, Yavor

AU - Vaquerizas, Juan M

AU - Cairns, Bradley

AU - Lenhard, Boris

AU - Müller, Ferenc

N1 - Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.

PY - 2021/3/8

Y1 - 2021/3/8

N2 - In many animal models, primordial germ cell (PGC) development depends on maternally deposited germ plasm, which prevents somatic cell fate. Here, we show that PGCs respond to regulatory information from the germ plasm in two distinct phases using two distinct mechanisms in zebrafish. We demonstrate that PGCs commence zygotic genome activation together with the somatic blastocysts with no demonstrable differences in transcriptional and chromatin opening. Unexpectedly, both PGC and somatic blastocysts activate germ-cell-specific genes, which are only stabilized in PGCs by cytoplasmic germ plasm determinants. Disaggregated perinuclear relocalization of germ plasm during PGC migration is regulated by the germ plasm determinant Tdrd7 and is coupled to dramatic divergence between PGC and somatic transcriptomes. This transcriptional divergence relies on PGC-specific cis-regulatory elements characterized by promoter-proximal distribution. We show that Tdrd7-dependent reconfiguration of chromatin accessibility is required for elaboration of PGC fate but not for PGC migration.

AB - In many animal models, primordial germ cell (PGC) development depends on maternally deposited germ plasm, which prevents somatic cell fate. Here, we show that PGCs respond to regulatory information from the germ plasm in two distinct phases using two distinct mechanisms in zebrafish. We demonstrate that PGCs commence zygotic genome activation together with the somatic blastocysts with no demonstrable differences in transcriptional and chromatin opening. Unexpectedly, both PGC and somatic blastocysts activate germ-cell-specific genes, which are only stabilized in PGCs by cytoplasmic germ plasm determinants. Disaggregated perinuclear relocalization of germ plasm during PGC migration is regulated by the germ plasm determinant Tdrd7 and is coupled to dramatic divergence between PGC and somatic transcriptomes. This transcriptional divergence relies on PGC-specific cis-regulatory elements characterized by promoter-proximal distribution. We show that Tdrd7-dependent reconfiguration of chromatin accessibility is required for elaboration of PGC fate but not for PGC migration.

U2 - 10.1016/j.devcel.2021.02.007

DO - 10.1016/j.devcel.2021.02.007

M3 - Article

C2 - 33651978

VL - 56

SP - 641-656.e5

JO - Developmental Cell

JF - Developmental Cell

SN - 1534-5807

IS - 5

ER -