Genome-wide Screening Identifies Phosphotransferase System Permease BepA to Be Involved in Enterococcus faecium Endocarditis and Biofilm Formation

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Genome-wide Screening Identifies Phosphotransferase System Permease BepA to Be Involved in Enterococcus faecium Endocarditis and Biofilm Formation. / Paganelli, Fernanda L; Huebner, Johannes; Singh, Kavindra V; Zhang, Xinglin; van Schaik, Willem; Wobser, Dominique; Braat, Johanna C; Murray, Barbara E; Bonten, Marc J M; Willems, Rob J L; Leavis, Helen L.

In: The Journal of Infectious Diseases, Vol. 214, No. 2, 15.07.2016, p. 189-95.

Research output: Contribution to journalArticlepeer-review

Harvard

Paganelli, FL, Huebner, J, Singh, KV, Zhang, X, van Schaik, W, Wobser, D, Braat, JC, Murray, BE, Bonten, MJM, Willems, RJL & Leavis, HL 2016, 'Genome-wide Screening Identifies Phosphotransferase System Permease BepA to Be Involved in Enterococcus faecium Endocarditis and Biofilm Formation', The Journal of Infectious Diseases, vol. 214, no. 2, pp. 189-95. https://doi.org/10.1093/infdis/jiw108

APA

Paganelli, F. L., Huebner, J., Singh, K. V., Zhang, X., van Schaik, W., Wobser, D., Braat, J. C., Murray, B. E., Bonten, M. J. M., Willems, R. J. L., & Leavis, H. L. (2016). Genome-wide Screening Identifies Phosphotransferase System Permease BepA to Be Involved in Enterococcus faecium Endocarditis and Biofilm Formation. The Journal of Infectious Diseases, 214(2), 189-95. https://doi.org/10.1093/infdis/jiw108

Vancouver

Author

Paganelli, Fernanda L ; Huebner, Johannes ; Singh, Kavindra V ; Zhang, Xinglin ; van Schaik, Willem ; Wobser, Dominique ; Braat, Johanna C ; Murray, Barbara E ; Bonten, Marc J M ; Willems, Rob J L ; Leavis, Helen L. / Genome-wide Screening Identifies Phosphotransferase System Permease BepA to Be Involved in Enterococcus faecium Endocarditis and Biofilm Formation. In: The Journal of Infectious Diseases. 2016 ; Vol. 214, No. 2. pp. 189-95.

Bibtex

@article{38b818f7fa004c66a0edf7fcf023365b,
title = "Genome-wide Screening Identifies Phosphotransferase System Permease BepA to Be Involved in Enterococcus faecium Endocarditis and Biofilm Formation",
abstract = "Enterococcus faecium is a common cause of nosocomial infections, of which infective endocarditis is associated with substantial mortality. In this study, we used a microarray-based transposon mapping (M-TraM) approach to evaluate a rat endocarditis model and identified a gene, originally annotated as {"}fruA{"} and renamed {"}bepA,{"} putatively encoding a carbohydrate phosphotransferase system (PTS) permease (biofilm and endocarditis-associated permease A [BepA]), as important in infective endocarditis. This gene is highly enriched in E. faecium clinical isolates and absent in commensal isolates that are not associated with infection. Confirmation of the phenotype was established in a competition experiment of wild-type and a markerless bepA mutant in a rat endocarditis model. In addition, deletion of bepA impaired biofilm formation in vitro in the presence of 100% human serum and metabolism of β-methyl-D-glucoside. β-glucoside metabolism has been linked to the metabolism of glycosaminoglycans that are exposed on injured heart valves, where bacteria attach and form vegetations. Therefore, we propose that the PTS permease BepA is directly implicated in E. faecium pathogenesis.",
keywords = "Journal Article",
author = "Paganelli, {Fernanda L} and Johannes Huebner and Singh, {Kavindra V} and Xinglin Zhang and {van Schaik}, Willem and Dominique Wobser and Braat, {Johanna C} and Murray, {Barbara E} and Bonten, {Marc J M} and Willems, {Rob J L} and Leavis, {Helen L}",
note = "{\textcopyright} The Author 2016. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail journals.permissions@oup.com.",
year = "2016",
month = jul,
day = "15",
doi = "10.1093/infdis/jiw108",
language = "English",
volume = "214",
pages = "189--95",
journal = "The Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "2",

}

RIS

TY - JOUR

T1 - Genome-wide Screening Identifies Phosphotransferase System Permease BepA to Be Involved in Enterococcus faecium Endocarditis and Biofilm Formation

AU - Paganelli, Fernanda L

AU - Huebner, Johannes

AU - Singh, Kavindra V

AU - Zhang, Xinglin

AU - van Schaik, Willem

AU - Wobser, Dominique

AU - Braat, Johanna C

AU - Murray, Barbara E

AU - Bonten, Marc J M

AU - Willems, Rob J L

AU - Leavis, Helen L

N1 - © The Author 2016. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail journals.permissions@oup.com.

PY - 2016/7/15

Y1 - 2016/7/15

N2 - Enterococcus faecium is a common cause of nosocomial infections, of which infective endocarditis is associated with substantial mortality. In this study, we used a microarray-based transposon mapping (M-TraM) approach to evaluate a rat endocarditis model and identified a gene, originally annotated as "fruA" and renamed "bepA," putatively encoding a carbohydrate phosphotransferase system (PTS) permease (biofilm and endocarditis-associated permease A [BepA]), as important in infective endocarditis. This gene is highly enriched in E. faecium clinical isolates and absent in commensal isolates that are not associated with infection. Confirmation of the phenotype was established in a competition experiment of wild-type and a markerless bepA mutant in a rat endocarditis model. In addition, deletion of bepA impaired biofilm formation in vitro in the presence of 100% human serum and metabolism of β-methyl-D-glucoside. β-glucoside metabolism has been linked to the metabolism of glycosaminoglycans that are exposed on injured heart valves, where bacteria attach and form vegetations. Therefore, we propose that the PTS permease BepA is directly implicated in E. faecium pathogenesis.

AB - Enterococcus faecium is a common cause of nosocomial infections, of which infective endocarditis is associated with substantial mortality. In this study, we used a microarray-based transposon mapping (M-TraM) approach to evaluate a rat endocarditis model and identified a gene, originally annotated as "fruA" and renamed "bepA," putatively encoding a carbohydrate phosphotransferase system (PTS) permease (biofilm and endocarditis-associated permease A [BepA]), as important in infective endocarditis. This gene is highly enriched in E. faecium clinical isolates and absent in commensal isolates that are not associated with infection. Confirmation of the phenotype was established in a competition experiment of wild-type and a markerless bepA mutant in a rat endocarditis model. In addition, deletion of bepA impaired biofilm formation in vitro in the presence of 100% human serum and metabolism of β-methyl-D-glucoside. β-glucoside metabolism has been linked to the metabolism of glycosaminoglycans that are exposed on injured heart valves, where bacteria attach and form vegetations. Therefore, we propose that the PTS permease BepA is directly implicated in E. faecium pathogenesis.

KW - Journal Article

U2 - 10.1093/infdis/jiw108

DO - 10.1093/infdis/jiw108

M3 - Article

C2 - 26984142

VL - 214

SP - 189

EP - 195

JO - The Journal of Infectious Diseases

JF - The Journal of Infectious Diseases

SN - 0022-1899

IS - 2

ER -