Genome complexity in acute lymphoblastic leukemia is revealed by array-based comparative genomic hybridization

Research output: Contribution to journalArticle

Standard

Genome complexity in acute lymphoblastic leukemia is revealed by array-based comparative genomic hybridization. / Strefford, JC; Worley, H; Barber, K; Wright, S; Stewart, Antony; Robinson, HM; Bettney, G; van Delft, FW; Atherton, MG; Davies, T; Griffiths, Michael; Hing, S; Ross, FM; Talley, P; Saha, V; Moorman, AV; Harrison, CJ.

In: Oncogene, Vol. 26, No. 29, 21.06.2007, p. 4306-4318.

Research output: Contribution to journalArticle

Harvard

Strefford, JC, Worley, H, Barber, K, Wright, S, Stewart, A, Robinson, HM, Bettney, G, van Delft, FW, Atherton, MG, Davies, T, Griffiths, M, Hing, S, Ross, FM, Talley, P, Saha, V, Moorman, AV & Harrison, CJ 2007, 'Genome complexity in acute lymphoblastic leukemia is revealed by array-based comparative genomic hybridization', Oncogene, vol. 26, no. 29, pp. 4306-4318. https://doi.org/10.1038/sj.onc.1210190

APA

Strefford, JC., Worley, H., Barber, K., Wright, S., Stewart, A., Robinson, HM., Bettney, G., van Delft, FW., Atherton, MG., Davies, T., Griffiths, M., Hing, S., Ross, FM., Talley, P., Saha, V., Moorman, AV., & Harrison, CJ. (2007). Genome complexity in acute lymphoblastic leukemia is revealed by array-based comparative genomic hybridization. Oncogene, 26(29), 4306-4318. https://doi.org/10.1038/sj.onc.1210190

Vancouver

Strefford JC, Worley H, Barber K, Wright S, Stewart A, Robinson HM et al. Genome complexity in acute lymphoblastic leukemia is revealed by array-based comparative genomic hybridization. Oncogene. 2007 Jun 21;26(29):4306-4318. https://doi.org/10.1038/sj.onc.1210190

Author

Strefford, JC ; Worley, H ; Barber, K ; Wright, S ; Stewart, Antony ; Robinson, HM ; Bettney, G ; van Delft, FW ; Atherton, MG ; Davies, T ; Griffiths, Michael ; Hing, S ; Ross, FM ; Talley, P ; Saha, V ; Moorman, AV ; Harrison, CJ. / Genome complexity in acute lymphoblastic leukemia is revealed by array-based comparative genomic hybridization. In: Oncogene. 2007 ; Vol. 26, No. 29. pp. 4306-4318.

Bibtex

@article{798aad1b2cbf4633bc1f6cb02ec4fb7b,
title = "Genome complexity in acute lymphoblastic leukemia is revealed by array-based comparative genomic hybridization",
abstract = "Chromosomal abnormalities are important for the classification and risk stratification of patients with acute lymphoblastic leukemia (ALL). However, approximately 30% of childhood and 50% of adult patients lack abnormalities with clinical relevance. Here, we describe the use of array-based comparative genomic hybridization (aCGH) to identify copy number alterations (CNA) in 58 ALL patients. CNA were identified in 83% of cases, and most frequently involved chromosomes 21 (n=42), 9 (n=21), 6 (n=16), 12 (n=11), 15 (n=11), 8 (n=10) and 17 (n=10). Deletions of 6q (del(6q)) were heterogeneous in size, in agreement with previous data, demonstrating the sensitivity of aCGH to measure CNA. Although 9p deletions showed considerable variability in both the extent and location, all encompassed the CDKN2A locus. Six patients showed del(12p), with a common region encompassing the ETV6 gene. Complex CNA were observed involving chromosomes 6 (n=2), 15 (n=2) and 21 (n=11) with multiple regions of loss and gain along each chromosome. Chromosome 21 CNA shared a common region of gain, with associated subtelomeric deletions. Other recurrent findings included dim(13q), dim(16q) and enh(17q). This is the first report of genome-wide detection of CNA in ALL patients using aCGH, and it has demonstrated a higher level of karyotype complexity than anticipated from conventional cytogenetic analysis.",
author = "JC Strefford and H Worley and K Barber and S Wright and Antony Stewart and HM Robinson and G Bettney and {van Delft}, FW and MG Atherton and T Davies and Michael Griffiths and S Hing and FM Ross and P Talley and V Saha and AV Moorman and CJ Harrison",
year = "2007",
month = jun,
day = "21",
doi = "10.1038/sj.onc.1210190",
language = "English",
volume = "26",
pages = "4306--4318",
journal = "Oncogene",
issn = "0950-9232",
publisher = "Nature Publishing Group",
number = "29",

}

RIS

TY - JOUR

T1 - Genome complexity in acute lymphoblastic leukemia is revealed by array-based comparative genomic hybridization

AU - Strefford, JC

AU - Worley, H

AU - Barber, K

AU - Wright, S

AU - Stewart, Antony

AU - Robinson, HM

AU - Bettney, G

AU - van Delft, FW

AU - Atherton, MG

AU - Davies, T

AU - Griffiths, Michael

AU - Hing, S

AU - Ross, FM

AU - Talley, P

AU - Saha, V

AU - Moorman, AV

AU - Harrison, CJ

PY - 2007/6/21

Y1 - 2007/6/21

N2 - Chromosomal abnormalities are important for the classification and risk stratification of patients with acute lymphoblastic leukemia (ALL). However, approximately 30% of childhood and 50% of adult patients lack abnormalities with clinical relevance. Here, we describe the use of array-based comparative genomic hybridization (aCGH) to identify copy number alterations (CNA) in 58 ALL patients. CNA were identified in 83% of cases, and most frequently involved chromosomes 21 (n=42), 9 (n=21), 6 (n=16), 12 (n=11), 15 (n=11), 8 (n=10) and 17 (n=10). Deletions of 6q (del(6q)) were heterogeneous in size, in agreement with previous data, demonstrating the sensitivity of aCGH to measure CNA. Although 9p deletions showed considerable variability in both the extent and location, all encompassed the CDKN2A locus. Six patients showed del(12p), with a common region encompassing the ETV6 gene. Complex CNA were observed involving chromosomes 6 (n=2), 15 (n=2) and 21 (n=11) with multiple regions of loss and gain along each chromosome. Chromosome 21 CNA shared a common region of gain, with associated subtelomeric deletions. Other recurrent findings included dim(13q), dim(16q) and enh(17q). This is the first report of genome-wide detection of CNA in ALL patients using aCGH, and it has demonstrated a higher level of karyotype complexity than anticipated from conventional cytogenetic analysis.

AB - Chromosomal abnormalities are important for the classification and risk stratification of patients with acute lymphoblastic leukemia (ALL). However, approximately 30% of childhood and 50% of adult patients lack abnormalities with clinical relevance. Here, we describe the use of array-based comparative genomic hybridization (aCGH) to identify copy number alterations (CNA) in 58 ALL patients. CNA were identified in 83% of cases, and most frequently involved chromosomes 21 (n=42), 9 (n=21), 6 (n=16), 12 (n=11), 15 (n=11), 8 (n=10) and 17 (n=10). Deletions of 6q (del(6q)) were heterogeneous in size, in agreement with previous data, demonstrating the sensitivity of aCGH to measure CNA. Although 9p deletions showed considerable variability in both the extent and location, all encompassed the CDKN2A locus. Six patients showed del(12p), with a common region encompassing the ETV6 gene. Complex CNA were observed involving chromosomes 6 (n=2), 15 (n=2) and 21 (n=11) with multiple regions of loss and gain along each chromosome. Chromosome 21 CNA shared a common region of gain, with associated subtelomeric deletions. Other recurrent findings included dim(13q), dim(16q) and enh(17q). This is the first report of genome-wide detection of CNA in ALL patients using aCGH, and it has demonstrated a higher level of karyotype complexity than anticipated from conventional cytogenetic analysis.

U2 - 10.1038/sj.onc.1210190

DO - 10.1038/sj.onc.1210190

M3 - Article

C2 - 17237825

VL - 26

SP - 4306

EP - 4318

JO - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 29

ER -