Generation of stem cell-based bioartificial anterior cruciate ligament (ACL) grafts for effective ACL rupture repair
Research output: Contribution to journal › Article › peer-review
Authors
Colleges, School and Institutes
External organisations
- 1. Department of Biomedical Research, Institute of Molecular Biology & Biotechnology, Foundation of Research and Technology-Hellas, University Campus, 45110 Ioannina, Greece
- Department of Pathology, University of Ioannina, 45110 Ioannina, Greece
- Centre of Clinical, Experimental Surgery and Translational Research, Experimental Surgery Unit, Biomedical Research Foundation of the Academy of Athens, 11527 Athens, Greece and 4. Attikon animal hospital, 19002 Paiania, Athens, Greece
- 5. Department of Biology, Electron microscopy laboratory, University of Crete, 70013 Heraklio, Greece
- 6. Orthopaedic Sports Medicine Center-Department of Orthopaedic Surgery, University of Ioannina, 45110 Ioannina, Greece
Abstract
In the present study, we combined stem cell technology with a non-absorbable biomaterial for the reconstruction of the ruptured ACL. Towards this purpose, multipotential stromal cells derived either from subcutaneous human adipose tissue (hAT-MSCs) or from induced pluripotent stem cells (iPSCs) generated from human foreskin fibroblasts (hiPSC-MSCs) were cultured on the biomaterial for 21 days in vitro to generate a 3D bioartifical ACL graft. Stem cell differentiation towards bone and ligament at the ends and central part of the biomaterial was selectively induced using either BMP-2/FGF-2 or TGF-β/FGF-2 combinations, respectively. The bioartificial ACL graft was subsequently implanted in a swine ACL rupture model in place of the surgically removed normal ACL. Four months post-implantation, the tissue engineered ACL graft generated an ACL-like tissue exhibiting morphological and biochemical characteristics resembling those of normal ACL.
Details
| Original language | English |
|---|---|
| Journal | Stem Cell Research |
| Early online date | 22 Apr 2016 |
| Publication status | E-pub ahead of print - 22 Apr 2016 |
Keywords
- human induced pluripotent stem cells, bioartificial ACL graft, tissue engineering, biomaterials, Anterior cruciate ligament reconstruction, Human adipose tissue multipotential stromal cells