Generation of liposomes to study the effect of mycobacterium tuberculosis lipids on HIV-1 cis- and trans-infections

Research output: Contribution to journalArticlepeer-review

Standard

Generation of liposomes to study the effect of mycobacterium tuberculosis lipids on HIV-1 cis- and trans-infections. / Pouget, Marion; Coussens, Anna K; Ruggiero, Alessandra; Koch, Anastasia; Thomas, Jordan; Besra, Gurdyal S; Wilkinson, Robert J; Bhatt, Apoorva; Pollakis, Georgios; Paxton, William A.

In: International Journal of Molecular Sciences, Vol. 22, No. 4, 1945, 16.02.2021.

Research output: Contribution to journalArticlepeer-review

Harvard

APA

Vancouver

Author

Pouget, Marion ; Coussens, Anna K ; Ruggiero, Alessandra ; Koch, Anastasia ; Thomas, Jordan ; Besra, Gurdyal S ; Wilkinson, Robert J ; Bhatt, Apoorva ; Pollakis, Georgios ; Paxton, William A. / Generation of liposomes to study the effect of mycobacterium tuberculosis lipids on HIV-1 cis- and trans-infections. In: International Journal of Molecular Sciences. 2021 ; Vol. 22, No. 4.

Bibtex

@article{47c43e7bf95b452e9b1c964b2316f11e,
title = "Generation of liposomes to study the effect of mycobacterium tuberculosis lipids on HIV-1 cis- and trans-infections",
abstract = "Tuberculosis (TB) is the leading cause of death among HIV-1-infected individuals and Mycobacterium tuberculosis (Mtb) co-infection is an early precipitate to AIDS. We aimed to determine whether Mtb strains differentially modulate cellular susceptibility to HIV-1 infection (cis- and trans-infection), via surface receptor interaction by their cell envelope lipids. Total lipids from pathogenic (lineage 4 Mtb H37Rv, CDC1551 and lineage 2 Mtb HN878, EU127) and non-pathogenic (Mycobacterium bovis BCG and Mycobacterium smegmatis) Mycobacterium strains were integrated into liposomes mimicking the lipid distribution and antigen accessibility of the mycobacterial cell wall. The resulting liposomes were tested for modulating in vitro HIV-1 cis- and trans-infection of TZM-bl cells using single-cycle infectious virus particles. Mtb glycolipids did not affect HIV-1 direct infection however, trans-infection of both R5 and X4 tropic HIV-1 strains were impaired in the presence of glycolipids from M. bovis, Mtb H37Rv and Mtb EU127 strains when using Raji-DC-SIGN cells or immature and mature dendritic cells (DCs) to capture virus. SL1, PDIM and TDM lipids were identified to be involved in DC-SIGN recognition and impairment of HIV-1 trans-infection. These findings indicate that variant strains of Mtb have differential effect on HIV-1 trans-infection with the potential to influence HIV-1 disease course in co-infected individuals.",
keywords = "HIV-1, TB, Mycobacterium tuberculosis, in vitro, DC-SIGN, liposomes, trans-infection, SL1, TDM, PDIM, H37Rv, HN878, CDC1551, EU127, BCG, M. smegmatis",
author = "Marion Pouget and Coussens, {Anna K} and Alessandra Ruggiero and Anastasia Koch and Jordan Thomas and Besra, {Gurdyal S} and Wilkinson, {Robert J} and Apoorva Bhatt and Georgios Pollakis and Paxton, {William A}",
year = "2021",
month = feb,
day = "16",
doi = "10.3390/ijms22041945",
language = "English",
volume = "22",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "MDPI",
number = "4",

}

RIS

TY - JOUR

T1 - Generation of liposomes to study the effect of mycobacterium tuberculosis lipids on HIV-1 cis- and trans-infections

AU - Pouget, Marion

AU - Coussens, Anna K

AU - Ruggiero, Alessandra

AU - Koch, Anastasia

AU - Thomas, Jordan

AU - Besra, Gurdyal S

AU - Wilkinson, Robert J

AU - Bhatt, Apoorva

AU - Pollakis, Georgios

AU - Paxton, William A

PY - 2021/2/16

Y1 - 2021/2/16

N2 - Tuberculosis (TB) is the leading cause of death among HIV-1-infected individuals and Mycobacterium tuberculosis (Mtb) co-infection is an early precipitate to AIDS. We aimed to determine whether Mtb strains differentially modulate cellular susceptibility to HIV-1 infection (cis- and trans-infection), via surface receptor interaction by their cell envelope lipids. Total lipids from pathogenic (lineage 4 Mtb H37Rv, CDC1551 and lineage 2 Mtb HN878, EU127) and non-pathogenic (Mycobacterium bovis BCG and Mycobacterium smegmatis) Mycobacterium strains were integrated into liposomes mimicking the lipid distribution and antigen accessibility of the mycobacterial cell wall. The resulting liposomes were tested for modulating in vitro HIV-1 cis- and trans-infection of TZM-bl cells using single-cycle infectious virus particles. Mtb glycolipids did not affect HIV-1 direct infection however, trans-infection of both R5 and X4 tropic HIV-1 strains were impaired in the presence of glycolipids from M. bovis, Mtb H37Rv and Mtb EU127 strains when using Raji-DC-SIGN cells or immature and mature dendritic cells (DCs) to capture virus. SL1, PDIM and TDM lipids were identified to be involved in DC-SIGN recognition and impairment of HIV-1 trans-infection. These findings indicate that variant strains of Mtb have differential effect on HIV-1 trans-infection with the potential to influence HIV-1 disease course in co-infected individuals.

AB - Tuberculosis (TB) is the leading cause of death among HIV-1-infected individuals and Mycobacterium tuberculosis (Mtb) co-infection is an early precipitate to AIDS. We aimed to determine whether Mtb strains differentially modulate cellular susceptibility to HIV-1 infection (cis- and trans-infection), via surface receptor interaction by their cell envelope lipids. Total lipids from pathogenic (lineage 4 Mtb H37Rv, CDC1551 and lineage 2 Mtb HN878, EU127) and non-pathogenic (Mycobacterium bovis BCG and Mycobacterium smegmatis) Mycobacterium strains were integrated into liposomes mimicking the lipid distribution and antigen accessibility of the mycobacterial cell wall. The resulting liposomes were tested for modulating in vitro HIV-1 cis- and trans-infection of TZM-bl cells using single-cycle infectious virus particles. Mtb glycolipids did not affect HIV-1 direct infection however, trans-infection of both R5 and X4 tropic HIV-1 strains were impaired in the presence of glycolipids from M. bovis, Mtb H37Rv and Mtb EU127 strains when using Raji-DC-SIGN cells or immature and mature dendritic cells (DCs) to capture virus. SL1, PDIM and TDM lipids were identified to be involved in DC-SIGN recognition and impairment of HIV-1 trans-infection. These findings indicate that variant strains of Mtb have differential effect on HIV-1 trans-infection with the potential to influence HIV-1 disease course in co-infected individuals.

KW - HIV-1

KW - TB

KW - Mycobacterium tuberculosis

KW - in vitro

KW - DC-SIGN

KW - liposomes

KW - trans-infection

KW - SL1

KW - TDM

KW - PDIM

KW - H37Rv

KW - HN878

KW - CDC1551

KW - EU127

KW - BCG

KW - M. smegmatis

U2 - 10.3390/ijms22041945

DO - 10.3390/ijms22041945

M3 - Article

C2 - 33669411

VL - 22

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 4

M1 - 1945

ER -