Generation of knockout rabbits using transcription activator-like effector nucleases

Research output: Contribution to journalArticle

Standard

Generation of knockout rabbits using transcription activator-like effector nucleases. / Wang, Yu; Fan, Nana; Song, Jun; Zhong, Juan; Guo, Xiaogang; Tian, Weihua; Zhang, Quanjun; Cui, Fenggong; Li, Li; Newsome, Philip N; Frampton, Jonathan; Esteban, Miguel A; Lai, Liangxue.

In: Cell Regeneration , Vol. 3, 3, 05.02.2014.

Research output: Contribution to journalArticle

Harvard

Wang, Y, Fan, N, Song, J, Zhong, J, Guo, X, Tian, W, Zhang, Q, Cui, F, Li, L, Newsome, PN, Frampton, J, Esteban, MA & Lai, L 2014, 'Generation of knockout rabbits using transcription activator-like effector nucleases', Cell Regeneration , vol. 3, 3. https://doi.org/10.1186/2045-9769-3-3

APA

Wang, Y., Fan, N., Song, J., Zhong, J., Guo, X., Tian, W., Zhang, Q., Cui, F., Li, L., Newsome, P. N., Frampton, J., Esteban, M. A., & Lai, L. (2014). Generation of knockout rabbits using transcription activator-like effector nucleases. Cell Regeneration , 3, [3]. https://doi.org/10.1186/2045-9769-3-3

Vancouver

Author

Wang, Yu ; Fan, Nana ; Song, Jun ; Zhong, Juan ; Guo, Xiaogang ; Tian, Weihua ; Zhang, Quanjun ; Cui, Fenggong ; Li, Li ; Newsome, Philip N ; Frampton, Jonathan ; Esteban, Miguel A ; Lai, Liangxue. / Generation of knockout rabbits using transcription activator-like effector nucleases. In: Cell Regeneration . 2014 ; Vol. 3.

Bibtex

@article{4a32af13ea2c4af3a338432392a446be,
title = "Generation of knockout rabbits using transcription activator-like effector nucleases",
abstract = "Zinc-finger nucleases and transcription activator-like effector nucleases are novel gene-editing platforms contributing to redefine the boundaries of modern biological research. They are composed of a non-specific cleavage domain and a tailor made DNA-binding module, which enables a broad range of genetic modifications by inducing efficient DNA double-strand breaks at desired loci. Among other remarkable uses, these nucleases have been employed to produce gene knockouts in mid-size and large animals, such as rabbits and pigs, respectively. This approach is cost effective, relatively quick, and can produce invaluable models for human disease studies, biotechnology or agricultural purposes. Here we describe a protocol for the efficient generation of knockout rabbits using transcription activator-like effector nucleases, and a perspective of the field.",
author = "Yu Wang and Nana Fan and Jun Song and Juan Zhong and Xiaogang Guo and Weihua Tian and Quanjun Zhang and Fenggong Cui and Li Li and Newsome, {Philip N} and Jonathan Frampton and Esteban, {Miguel A} and Liangxue Lai",
year = "2014",
month = feb,
day = "5",
doi = "10.1186/2045-9769-3-3",
language = "English",
volume = "3",
journal = "Cell Regeneration ",
issn = "2045-9769",
publisher = "BioMed Central Ltd",

}

RIS

TY - JOUR

T1 - Generation of knockout rabbits using transcription activator-like effector nucleases

AU - Wang, Yu

AU - Fan, Nana

AU - Song, Jun

AU - Zhong, Juan

AU - Guo, Xiaogang

AU - Tian, Weihua

AU - Zhang, Quanjun

AU - Cui, Fenggong

AU - Li, Li

AU - Newsome, Philip N

AU - Frampton, Jonathan

AU - Esteban, Miguel A

AU - Lai, Liangxue

PY - 2014/2/5

Y1 - 2014/2/5

N2 - Zinc-finger nucleases and transcription activator-like effector nucleases are novel gene-editing platforms contributing to redefine the boundaries of modern biological research. They are composed of a non-specific cleavage domain and a tailor made DNA-binding module, which enables a broad range of genetic modifications by inducing efficient DNA double-strand breaks at desired loci. Among other remarkable uses, these nucleases have been employed to produce gene knockouts in mid-size and large animals, such as rabbits and pigs, respectively. This approach is cost effective, relatively quick, and can produce invaluable models for human disease studies, biotechnology or agricultural purposes. Here we describe a protocol for the efficient generation of knockout rabbits using transcription activator-like effector nucleases, and a perspective of the field.

AB - Zinc-finger nucleases and transcription activator-like effector nucleases are novel gene-editing platforms contributing to redefine the boundaries of modern biological research. They are composed of a non-specific cleavage domain and a tailor made DNA-binding module, which enables a broad range of genetic modifications by inducing efficient DNA double-strand breaks at desired loci. Among other remarkable uses, these nucleases have been employed to produce gene knockouts in mid-size and large animals, such as rabbits and pigs, respectively. This approach is cost effective, relatively quick, and can produce invaluable models for human disease studies, biotechnology or agricultural purposes. Here we describe a protocol for the efficient generation of knockout rabbits using transcription activator-like effector nucleases, and a perspective of the field.

U2 - 10.1186/2045-9769-3-3

DO - 10.1186/2045-9769-3-3

M3 - Article

C2 - 25408882

VL - 3

JO - Cell Regeneration

JF - Cell Regeneration

SN - 2045-9769

M1 - 3

ER -