Generation of human induced pluripotent stem cells in defined, feeder-free conditions

Athena Kyrkou; Dimitris Stellas; Marika Syrrou; Apostolos Klinakis; Theodore Fotsis; Carol Murphy

doi:10.1016/j.scr.2016.05.006

Generation of human induced pluripotent stem cells in defined, feeder-free conditions

Athena Kyrkou, Dimitris Stellas, Marika Syrrou, Apostolos Klinakis, Theodore Fotsis, Carol Murphy

Biosciences

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Abstract

Herein, we describe a modified protocol for the generation of human induced pluripotent stem cells (hiPS) and expansion under defined, serum free and feeder free conditions. These cells exhibit a high level of plasticity towards various differentiation pathways both in vitro and in vivo. Ultimately, hiPS-derived lines
achieved high standards of three dimensional differentiations on biomaterial scaffolds and promoted in vivo regeneration of complex organs, such as Anterior Cruciate Ligament (in swine ACL-rupture models) and other tissues as well.

Original language	English
Journal	Stem Cell Research
Early online date	12 May 2016
DOIs	https://doi.org/10.1016/j.scr.2016.05.006
Publication status	E-pub ahead of print - 12 May 2016

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title = "Generation of human induced pluripotent stem cells in defined, feeder-free conditions",

abstract = "Herein, we describe a modified protocol for the generation of human induced pluripotent stem cells (hiPS) and expansion under defined, serum free and feeder free conditions. These cells exhibit a high level of plasticity towards various differentiation pathways both in vitro and in vivo. Ultimately, hiPS-derived linesachieved high standards of three dimensional differentiations on biomaterial scaffolds and promoted in vivo regeneration of complex organs, such as Anterior Cruciate Ligament (in swine ACL-rupture models) and other tissues as well.",

author = "Athena Kyrkou and Dimitris Stellas and Marika Syrrou and Apostolos Klinakis and Theodore Fotsis and Carol Murphy",

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AU - Kyrkou, Athena

AU - Stellas, Dimitris

AU - Syrrou, Marika

AU - Klinakis, Apostolos

AU - Fotsis, Theodore

AU - Murphy, Carol

PY - 2016/5/12

Y1 - 2016/5/12

N2 - Herein, we describe a modified protocol for the generation of human induced pluripotent stem cells (hiPS) and expansion under defined, serum free and feeder free conditions. These cells exhibit a high level of plasticity towards various differentiation pathways both in vitro and in vivo. Ultimately, hiPS-derived linesachieved high standards of three dimensional differentiations on biomaterial scaffolds and promoted in vivo regeneration of complex organs, such as Anterior Cruciate Ligament (in swine ACL-rupture models) and other tissues as well.

AB - Herein, we describe a modified protocol for the generation of human induced pluripotent stem cells (hiPS) and expansion under defined, serum free and feeder free conditions. These cells exhibit a high level of plasticity towards various differentiation pathways both in vitro and in vivo. Ultimately, hiPS-derived linesachieved high standards of three dimensional differentiations on biomaterial scaffolds and promoted in vivo regeneration of complex organs, such as Anterior Cruciate Ligament (in swine ACL-rupture models) and other tissues as well.

U2 - 10.1016/j.scr.2016.05.006

DO - 10.1016/j.scr.2016.05.006

M3 - Article

SN - 1873-5061

JO - Stem Cell Research

JF - Stem Cell Research

ER -

Generation of human induced pluripotent stem cells in defined, feeder-free conditions

Abstract

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