Generation of human induced pluripotent stem cells in defined, feeder-free conditions

Research output: Contribution to journalArticlepeer-review


  • Athena Kyrkou
  • Dimitris Stellas
  • Marika Syrrou
  • Apostolos Klinakis
  • Theodore Fotsis

Colleges, School and Institutes

External organisations

  • Centre for Basic Research, Biomedical Research Foundation of the Academy of Athens, 11527 Athens, Greece
  • Laboratory of Biology, Medical School, University of Ioannina, 45110 Ioannina, Greece
  • 1. Department of Biomedical Research, Institute of Molecular Biology & Biotechnology, Foundation of Research and Technology-Hellas, University Campus, 45110 Ioannina, Greece
  • Laboratory of Biological Chemistry, Medical School, University of Ioannina, 45110 Ioannina, Greece
  • School of Biosciences, University of Birmingham


Herein, we describe a modified protocol for the generation of human induced pluripotent stem cells (hiPS) and expansion under defined, serum free and feeder free conditions. These cells exhibit a high level of plasticity towards various differentiation pathways both in vitro and in vivo. Ultimately, hiPS-derived lines
achieved high standards of three dimensional differentiations on biomaterial scaffolds and promoted in vivo regeneration of complex organs, such as Anterior Cruciate Ligament (in swine ACL-rupture models) and other tissues as well.


Original languageEnglish
JournalStem Cell Research
Early online date12 May 2016
Publication statusE-pub ahead of print - 12 May 2016