Generation of a selective 5-HT_3B subunit-recognising polyclonal antibody; identification of immunoreactive cells in rat hippocampus

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Generation of a selective 5-HT_3B subunit-recognising polyclonal antibody; identification of immunoreactive cells in rat hippocampus. / Monk, SA; Desai, K; Brady, CA; Williams, Julie; Lin, L; Princivalle, A; Hope, A; Barnes, Nicholas.

In: Neuropharmacology, Vol. 41, No. 8, 01.12.2001, p. 1013-1016.

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@article{5216e68fb5cf40d2b4e1a07b436c346d,
title = "Generation of a selective 5-HT_3B subunit-recognising polyclonal antibody; identification of immunoreactive cells in rat hippocampus",
abstract = "The present study generated a polyclonal antibody (AP86/3) that recognises a peptide sequence of the h5-HT(3B) receptor subunit. Western blot analysis of homogenates prepared from cell lines expressing either homomeric (h5-HT(3A)) or heteromeric (h5-HT(3A/3B)) receptors, as well as immunocytochemical studies with the same cell lines, indicated that AP86/3 recognised, selectively, the 5-HT(3B) subunit. Immunohistochemical labelling was also apparent in cells in the rat hippocampus that displayed the distribution and morphology of interneurones.",
keywords = "5-HT3 receptor, hippocampus, 5-HT3B subunit, ligand-gated ion channel",
author = "SA Monk and K Desai and CA Brady and Julie Williams and L Lin and A Princivalle and A Hope and Nicholas Barnes",
year = "2001",
month = dec,
day = "1",
doi = "10.1016/S0028-3908(01)00153-8",
language = "English",
volume = "41",
pages = "1013--1016",
journal = "Neuropharmacology",
issn = "0028-3908",
publisher = "Elsevier",
number = "8",

}

RIS

TY - JOUR

T1 - Generation of a selective 5-HT_3B subunit-recognising polyclonal antibody; identification of immunoreactive cells in rat hippocampus

AU - Monk, SA

AU - Desai, K

AU - Brady, CA

AU - Williams, Julie

AU - Lin, L

AU - Princivalle, A

AU - Hope, A

AU - Barnes, Nicholas

PY - 2001/12/1

Y1 - 2001/12/1

N2 - The present study generated a polyclonal antibody (AP86/3) that recognises a peptide sequence of the h5-HT(3B) receptor subunit. Western blot analysis of homogenates prepared from cell lines expressing either homomeric (h5-HT(3A)) or heteromeric (h5-HT(3A/3B)) receptors, as well as immunocytochemical studies with the same cell lines, indicated that AP86/3 recognised, selectively, the 5-HT(3B) subunit. Immunohistochemical labelling was also apparent in cells in the rat hippocampus that displayed the distribution and morphology of interneurones.

AB - The present study generated a polyclonal antibody (AP86/3) that recognises a peptide sequence of the h5-HT(3B) receptor subunit. Western blot analysis of homogenates prepared from cell lines expressing either homomeric (h5-HT(3A)) or heteromeric (h5-HT(3A/3B)) receptors, as well as immunocytochemical studies with the same cell lines, indicated that AP86/3 recognised, selectively, the 5-HT(3B) subunit. Immunohistochemical labelling was also apparent in cells in the rat hippocampus that displayed the distribution and morphology of interneurones.

KW - 5-HT3 receptor

KW - hippocampus

KW - 5-HT3B subunit

KW - ligand-gated ion channel

UR - http://www.scopus.com/inward/record.url?scp=0035666156&partnerID=8YFLogxK

U2 - 10.1016/S0028-3908(01)00153-8

DO - 10.1016/S0028-3908(01)00153-8

M3 - Article

C2 - 11747906

VL - 41

SP - 1013

EP - 1016

JO - Neuropharmacology

JF - Neuropharmacology

SN - 0028-3908

IS - 8

ER -