Generation of a selective 5-HT_3B subunit-recognising polyclonal antibody; identification of immunoreactive cells in rat hippocampus
Research output: Contribution to journal › Article
The present study generated a polyclonal antibody (AP86/3) that recognises a peptide sequence of the h5-HT(3B) receptor subunit. Western blot analysis of homogenates prepared from cell lines expressing either homomeric (h5-HT(3A)) or heteromeric (h5-HT(3A/3B)) receptors, as well as immunocytochemical studies with the same cell lines, indicated that AP86/3 recognised, selectively, the 5-HT(3B) subunit. Immunohistochemical labelling was also apparent in cells in the rat hippocampus that displayed the distribution and morphology of interneurones.
|Number of pages||4|
|Publication status||Published - 1 Dec 2001|
- 5-HT3 receptor, hippocampus, 5-HT3B subunit, ligand-gated ion channel