Generation of a selective 5-HT_3B subunit-recognising polyclonal antibody; identification of immunoreactive cells in rat hippocampus

Research output: Contribution to journalArticle

Authors

  • SA Monk
  • K Desai
  • CA Brady
  • L Lin
  • A Princivalle
  • A Hope

Colleges, School and Institutes

Abstract

The present study generated a polyclonal antibody (AP86/3) that recognises a peptide sequence of the h5-HT(3B) receptor subunit. Western blot analysis of homogenates prepared from cell lines expressing either homomeric (h5-HT(3A)) or heteromeric (h5-HT(3A/3B)) receptors, as well as immunocytochemical studies with the same cell lines, indicated that AP86/3 recognised, selectively, the 5-HT(3B) subunit. Immunohistochemical labelling was also apparent in cells in the rat hippocampus that displayed the distribution and morphology of interneurones.

Details

Original languageEnglish
Pages (from-to)1013-1016
Number of pages4
JournalNeuropharmacology
Volume41
Issue number8
Publication statusPublished - 1 Dec 2001

Keywords

  • 5-HT3 receptor, hippocampus, 5-HT3B subunit, ligand-gated ion channel