Generation of a selective 5-HT_3B subunit-recognising polyclonal antibody; identification of immunoreactive cells in rat hippocampus

SA Monk; K Desai; CA Brady; Julie Williams; L Lin; A Princivalle; A Hope; Nicholas Barnes

doi:10.1016/S0028-3908(01)00153-8

Generation of a selective 5-HT_3B subunit-recognising polyclonal antibody; identification of immunoreactive cells in rat hippocampus

SA Monk, K Desai, CA Brady, Julie Williams, L Lin, A Princivalle, A Hope, Nicholas Barnes

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Abstract

The present study generated a polyclonal antibody (AP86/3) that recognises a peptide sequence of the h5-HT(3B) receptor subunit. Western blot analysis of homogenates prepared from cell lines expressing either homomeric (h5-HT(3A)) or heteromeric (h5-HT(3A/3B)) receptors, as well as immunocytochemical studies with the same cell lines, indicated that AP86/3 recognised, selectively, the 5-HT(3B) subunit. Immunohistochemical labelling was also apparent in cells in the rat hippocampus that displayed the distribution and morphology of interneurones.

Original language	English
Pages (from-to)	1013-1016
Number of pages	4
Journal	Neuropharmacology
Volume	41
Issue number	8
DOIs	https://doi.org/10.1016/S0028-3908(01)00153-8
Publication status	Published - 1 Dec 2001

Keywords

5-HT3 receptor
hippocampus
5-HT3B subunit
ligand-gated ion channel

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10.1016/S0028-3908(01)00153-8

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abstract = "The present study generated a polyclonal antibody (AP86/3) that recognises a peptide sequence of the h5-HT(3B) receptor subunit. Western blot analysis of homogenates prepared from cell lines expressing either homomeric (h5-HT(3A)) or heteromeric (h5-HT(3A/3B)) receptors, as well as immunocytochemical studies with the same cell lines, indicated that AP86/3 recognised, selectively, the 5-HT(3B) subunit. Immunohistochemical labelling was also apparent in cells in the rat hippocampus that displayed the distribution and morphology of interneurones.",

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T1 - Generation of a selective 5-HT_3B subunit-recognising polyclonal antibody; identification of immunoreactive cells in rat hippocampus

AU - Monk, SA

AU - Desai, K

AU - Brady, CA

AU - Williams, Julie

AU - Lin, L

AU - Princivalle, A

AU - Hope, A

AU - Barnes, Nicholas

PY - 2001/12/1

Y1 - 2001/12/1

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AB - The present study generated a polyclonal antibody (AP86/3) that recognises a peptide sequence of the h5-HT(3B) receptor subunit. Western blot analysis of homogenates prepared from cell lines expressing either homomeric (h5-HT(3A)) or heteromeric (h5-HT(3A/3B)) receptors, as well as immunocytochemical studies with the same cell lines, indicated that AP86/3 recognised, selectively, the 5-HT(3B) subunit. Immunohistochemical labelling was also apparent in cells in the rat hippocampus that displayed the distribution and morphology of interneurones.

KW - 5-HT3 receptor

KW - hippocampus

KW - 5-HT3B subunit

KW - ligand-gated ion channel

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JO - Neuropharmacology

JF - Neuropharmacology

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ER -

Generation of a selective 5-HT_3B subunit-recognising polyclonal antibody; identification of immunoreactive cells in rat hippocampus

Abstract

Keywords

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