Generation and characterization of novel stromal specific antibodies

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@article{beac8c21a857433e9b018ad445fe4bb0,
title = "Generation and characterization of novel stromal specific antibodies",
abstract = "Rheumatoid synovial fibroblasts were used as an immunogen to produce monoclonal antibodies selected for their reactivity with stromal cell antigens. Mice were immunised with low passage whole cell preparations and the subsequent hybridomas were screened by immunohistochemistry on rheumatoid synovium and tonsil sections. The aim was to identify those antibodies that recognised antigens that were restricted to stromal cells and were not expressed on CD45 positive leucocytes. A significant number of antibodies detected antigen that identified endothelial cells. These antibodies were further characterised to determine whether the vessels identified by these antibodies were vascular or lymphatic. From five fusions clones were identified with predominant reactivity with: 1) fibroblasts and endothelial cells; or 2) broad stromal elements (fibroblast, endothelium, epithelium, follicular dendritic cells). A fibroblast-specific antibody that did not also identify vessels was not generated. Examples of each reactivity pattern are discussed.",
author = "Sapna Halder and Deborah Hardie and Dagmar Scheel-Toellner and Michael Salmon and Christopher Buckley",
year = "2005",
month = sep,
day = "1",
doi = "10.1038/sj.cr.7290344",
language = "English",
volume = "15",
pages = "739--744",
journal = "Cell Research",
issn = "1001-0602",
publisher = "Nature Publishing Group",
number = "9",

}

RIS

TY - JOUR

T1 - Generation and characterization of novel stromal specific antibodies

AU - Halder, Sapna

AU - Hardie, Deborah

AU - Scheel-Toellner, Dagmar

AU - Salmon, Michael

AU - Buckley, Christopher

PY - 2005/9/1

Y1 - 2005/9/1

N2 - Rheumatoid synovial fibroblasts were used as an immunogen to produce monoclonal antibodies selected for their reactivity with stromal cell antigens. Mice were immunised with low passage whole cell preparations and the subsequent hybridomas were screened by immunohistochemistry on rheumatoid synovium and tonsil sections. The aim was to identify those antibodies that recognised antigens that were restricted to stromal cells and were not expressed on CD45 positive leucocytes. A significant number of antibodies detected antigen that identified endothelial cells. These antibodies were further characterised to determine whether the vessels identified by these antibodies were vascular or lymphatic. From five fusions clones were identified with predominant reactivity with: 1) fibroblasts and endothelial cells; or 2) broad stromal elements (fibroblast, endothelium, epithelium, follicular dendritic cells). A fibroblast-specific antibody that did not also identify vessels was not generated. Examples of each reactivity pattern are discussed.

AB - Rheumatoid synovial fibroblasts were used as an immunogen to produce monoclonal antibodies selected for their reactivity with stromal cell antigens. Mice were immunised with low passage whole cell preparations and the subsequent hybridomas were screened by immunohistochemistry on rheumatoid synovium and tonsil sections. The aim was to identify those antibodies that recognised antigens that were restricted to stromal cells and were not expressed on CD45 positive leucocytes. A significant number of antibodies detected antigen that identified endothelial cells. These antibodies were further characterised to determine whether the vessels identified by these antibodies were vascular or lymphatic. From five fusions clones were identified with predominant reactivity with: 1) fibroblasts and endothelial cells; or 2) broad stromal elements (fibroblast, endothelium, epithelium, follicular dendritic cells). A fibroblast-specific antibody that did not also identify vessels was not generated. Examples of each reactivity pattern are discussed.

UR - http://www.scopus.com/inward/record.url?scp=27944467186&partnerID=8YFLogxK

U2 - 10.1038/sj.cr.7290344

DO - 10.1038/sj.cr.7290344

M3 - Article

C2 - 16212881

VL - 15

SP - 739

EP - 744

JO - Cell Research

JF - Cell Research

SN - 1001-0602

IS - 9

ER -