Gene expression profiles of dynamically compressed single chondrocytes and chondrons

Research output: Contribution to journalArticle

Authors

  • QG Wang
  • JL Magnay
  • B Nguyen
  • AJ El Haj
  • NJ Kuiper

Colleges, School and Institutes

Abstract

A chondrocyte produces a hydrated pericellular matrix (PCM); together they form a chondron. Previous work has shown that the presence of the PCM influences the biological response of chondrocytes to loading. The objective of this study was to determine the gene expression profiles of enzymatically isolated single chondrocytes and chondrons in response to dynamic compression. Cartilage specific extracellular matrix components and transcription factors were examined. Following dynamic compression, chondrocytes and chondrons showed variations in gene expression profiles. Aggrecan, Type II collagen and osteopontin gene expression were significantly increased in chondrons. Lubricin gene expression decreased in both chondrons and chondrocytes. Dynamic compression had no effect on SOX9 gene expression. Our results demonstrate a clear role for the PCM in interfacing the mechanical signalling in chondrocytes in response to dynamic compression. Further investigation of single chondrocytes and chondrons from different zones within articular cartilage may further our understanding of cartilage mechanobiology. (C) 2009 Published by Elsevier Inc.

Details

Original languageEnglish
Pages (from-to)738-742
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume379
Issue number3
Publication statusPublished - 1 Feb 2009

Keywords

  • Chondrons, Micromanipulation, Dynamic compression, Cartilage, Chondrocytes, Cyclic loading, Tissue engineering, Single cell quantitative PCR, Biomechanical properties, Pericellular matrix