Projects per year
Abstract
Collagen activates mammalian platelets through a complex of the immunoglobulin (Ig) receptor GPVI and the Fc receptor γ-chain, which has an immunoreceptor tyrosine-based activation motif (ITAM). Cross-linking of GPVI mediates activation through the sequential activation of Src and Syk family kinases and activation of PLCγ2. Nucleated thrombocytes in fish are activated by collagen but lack an ortholog of GPVI. In this study we show that collagen activates trout thrombocytes in whole blood and under flow conditions through a Src kinase driven pathway. We identify the Ig receptor G6f-like as a collagen receptor and demonstrate in a cell line assay that it signals through its cytoplasmic ITAM. Using a morpholino for in vivo knock-down of G6f-like levels in zebrafish, we observed a marked delay or absence of occlusion of the venous and arterial systems in response to laser injury. Thus, G6f-like is a physiologically relevant collagen receptor in fish thrombocytes which signals through the same ITAM-based signalling pathway as mammalian GPVI, providing a novel example of convergent evolution.
Original language | English |
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Article number | e52622 |
Journal | PLoS ONE |
Volume | 7 |
Issue number | 12 |
DOIs | |
Publication status | Published - 21 Dec 2012 |
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Dive into the research topics of 'G6f-like is an ITAM-containing collagen receptor in thrombocytes'. Together they form a unique fingerprint.Projects
- 6 Finished
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The Early Events in Signalling by Platelet ITAM and ITAM-Like Receptors
1/08/09 → 31/03/16
Project: Research
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Dissection of the Proximal Events in the CLEC-2 Signalling Cascade
1/03/08 → 21/08/11
Project: Research
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Studies on Three Novel Platelet Membrane Signalling Proteins
Watson, S., Frampton, J., Senis, Y. & Tomlinson, M.
1/08/06 → 31/07/09
Project: Research
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Signalling by ITAM and Integrin Receptors in Platelets
THE WELLCOME TRUST, UHB CHARITABLE FUNDS
1/05/04 → 31/10/09
Project: Research