Functional analysis of the finO distal region of plasmid R1

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Functional analysis of the finO distal region of plasmid R1. / Nuk, Monika R; Reisner, Andreas; Neuwirth, Martina; Schilcher, Katrin; Arnold, Roland; Jehl, Andre; Rattei, Thomas; Zechner, Ellen L.

In: Plasmid, Vol. 65, No. 2, 03.2011, p. 159-68.

Research output: Contribution to journalArticle

Harvard

Nuk, MR, Reisner, A, Neuwirth, M, Schilcher, K, Arnold, R, Jehl, A, Rattei, T & Zechner, EL 2011, 'Functional analysis of the finO distal region of plasmid R1', Plasmid, vol. 65, no. 2, pp. 159-68. https://doi.org/10.1016/j.plasmid.2010.12.002

APA

Nuk, M. R., Reisner, A., Neuwirth, M., Schilcher, K., Arnold, R., Jehl, A., Rattei, T., & Zechner, E. L. (2011). Functional analysis of the finO distal region of plasmid R1. Plasmid, 65(2), 159-68. https://doi.org/10.1016/j.plasmid.2010.12.002

Vancouver

Nuk MR, Reisner A, Neuwirth M, Schilcher K, Arnold R, Jehl A et al. Functional analysis of the finO distal region of plasmid R1. Plasmid. 2011 Mar;65(2):159-68. https://doi.org/10.1016/j.plasmid.2010.12.002

Author

Nuk, Monika R ; Reisner, Andreas ; Neuwirth, Martina ; Schilcher, Katrin ; Arnold, Roland ; Jehl, Andre ; Rattei, Thomas ; Zechner, Ellen L. / Functional analysis of the finO distal region of plasmid R1. In: Plasmid. 2011 ; Vol. 65, No. 2. pp. 159-68.

Bibtex

@article{a4cd12f641d0480a8b774f5e48b85a9f,
title = "Functional analysis of the finO distal region of plasmid R1",
abstract = "The intergenic region linking conjugative transfer and replication copy control modules of IncF plasmids shows conservation of gene homology and organization. Genes distal to finO are coordinately expressed with the upstream transfer operon encoding the majority of conjugation genes in related plasmids. Here we investigate potential functions for these genes in copy number control and in processes related to conjugation: gene transfer, pilus specific phage infection and plasmid-promoted biofilm formation by an Escherichia coli host. We find that insertional inactivation of genes in the finO distal region reduced transcriptional read through into the downstream copB gene of plasmid R1. The mutant plasmid derivatives exhibited a reduced copy number compared to the wild type. Moreover all insertion mutant derivatives of plasmid R1-16 with aberrantly low copy numbers conferred poor biofilm forming ability to their hosts. The general mutagenesis thus identified plasmid stability genes as the only plasmid functions besides conjugation genes linked to plasmid-promoted biofilm production under these laboratory conditions. Our findings imply that a novel component of cis- or trans-regulation on the transcriptional level is important to normal R1 plasmid copy number regulation.",
keywords = "Biofilms, Escherichia coli, Escherichia coli Proteins, Gene Dosage, Gene Expression Regulation, Bacterial, Gene Order, Molecular Sequence Data, Mutagenesis, Mutagenesis, Insertional, Mutation, Phenotype, R Factors, RNA-Binding Proteins, Repressor Proteins, Journal Article, Research Support, Non-U.S. Gov't",
author = "Nuk, {Monika R} and Andreas Reisner and Martina Neuwirth and Katrin Schilcher and Roland Arnold and Andre Jehl and Thomas Rattei and Zechner, {Ellen L}",
note = "Copyright {\textcopyright} 2010 Elsevier Inc. All rights reserved.",
year = "2011",
month = mar,
doi = "10.1016/j.plasmid.2010.12.002",
language = "English",
volume = "65",
pages = "159--68",
journal = "Plasmid",
issn = "0147-619X",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Functional analysis of the finO distal region of plasmid R1

AU - Nuk, Monika R

AU - Reisner, Andreas

AU - Neuwirth, Martina

AU - Schilcher, Katrin

AU - Arnold, Roland

AU - Jehl, Andre

AU - Rattei, Thomas

AU - Zechner, Ellen L

N1 - Copyright © 2010 Elsevier Inc. All rights reserved.

PY - 2011/3

Y1 - 2011/3

N2 - The intergenic region linking conjugative transfer and replication copy control modules of IncF plasmids shows conservation of gene homology and organization. Genes distal to finO are coordinately expressed with the upstream transfer operon encoding the majority of conjugation genes in related plasmids. Here we investigate potential functions for these genes in copy number control and in processes related to conjugation: gene transfer, pilus specific phage infection and plasmid-promoted biofilm formation by an Escherichia coli host. We find that insertional inactivation of genes in the finO distal region reduced transcriptional read through into the downstream copB gene of plasmid R1. The mutant plasmid derivatives exhibited a reduced copy number compared to the wild type. Moreover all insertion mutant derivatives of plasmid R1-16 with aberrantly low copy numbers conferred poor biofilm forming ability to their hosts. The general mutagenesis thus identified plasmid stability genes as the only plasmid functions besides conjugation genes linked to plasmid-promoted biofilm production under these laboratory conditions. Our findings imply that a novel component of cis- or trans-regulation on the transcriptional level is important to normal R1 plasmid copy number regulation.

AB - The intergenic region linking conjugative transfer and replication copy control modules of IncF plasmids shows conservation of gene homology and organization. Genes distal to finO are coordinately expressed with the upstream transfer operon encoding the majority of conjugation genes in related plasmids. Here we investigate potential functions for these genes in copy number control and in processes related to conjugation: gene transfer, pilus specific phage infection and plasmid-promoted biofilm formation by an Escherichia coli host. We find that insertional inactivation of genes in the finO distal region reduced transcriptional read through into the downstream copB gene of plasmid R1. The mutant plasmid derivatives exhibited a reduced copy number compared to the wild type. Moreover all insertion mutant derivatives of plasmid R1-16 with aberrantly low copy numbers conferred poor biofilm forming ability to their hosts. The general mutagenesis thus identified plasmid stability genes as the only plasmid functions besides conjugation genes linked to plasmid-promoted biofilm production under these laboratory conditions. Our findings imply that a novel component of cis- or trans-regulation on the transcriptional level is important to normal R1 plasmid copy number regulation.

KW - Biofilms

KW - Escherichia coli

KW - Escherichia coli Proteins

KW - Gene Dosage

KW - Gene Expression Regulation, Bacterial

KW - Gene Order

KW - Molecular Sequence Data

KW - Mutagenesis

KW - Mutagenesis, Insertional

KW - Mutation

KW - Phenotype

KW - R Factors

KW - RNA-Binding Proteins

KW - Repressor Proteins

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.plasmid.2010.12.002

DO - 10.1016/j.plasmid.2010.12.002

M3 - Article

C2 - 21145347

VL - 65

SP - 159

EP - 168

JO - Plasmid

JF - Plasmid

SN - 0147-619X

IS - 2

ER -