Function of CD4+CD3- cells in relation to B and T zone stroma in spleen

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@article{b618fa01d5d04b28961069adb6482d78,
title = "Function of CD4+CD3- cells in relation to B and T zone stroma in spleen",
abstract = "Lymphocytes from lymphotoxin (LT) alpha-deficient mice, which lack segregation of their B- and T-cell areas, acquire normal organization following adoptive transfer into RAG-deficient recipients, identifying a non-B non-T cell in the segregation process. Here we show that a CD4+CD3- accessory cell is tightly associated with discrete VCAM-1-expressing stromal cells in B- and T-cell areas of the mouse spleen. CD4+CD3- cells express high levels of LTalpha, LTbeta, and tumor necrosis factor (TNF) alpha, which are the ligands for the LTbeta receptor and TNFR1 expressed by stromal cells. The expression of these ligands is functional, as transferring CD4+CD3- cells derived from either embryonic or adult tissues into LTalpha-deficient mice organizes B/T segregation and up-regulates CCL21 protein expression in areas where T cells are segregated from B cells. We propose that the function of CD4+CD3- cells is to form a link between primed CD4 T cells and the underlying stromal elements, creating distinct microenvironments in which they enable effector responses.",
author = "Mi-Yeon Kim and Fiona McConnell and Fabrina Gaspal and Andrea White and Stephanie Glanville and Vasileios Bekiaris and Lucy Walker and Jorge Caamano and Eric Jenkinson and Graham Anderson and Peter Lane",
year = "2007",
month = feb,
day = "15",
doi = "10.1182/blood-2006-04-018465",
language = "English",
volume = "109",
pages = "1602--10",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "4",

}

RIS

TY - JOUR

T1 - Function of CD4+CD3- cells in relation to B and T zone stroma in spleen

AU - Kim, Mi-Yeon

AU - McConnell, Fiona

AU - Gaspal, Fabrina

AU - White, Andrea

AU - Glanville, Stephanie

AU - Bekiaris, Vasileios

AU - Walker, Lucy

AU - Caamano, Jorge

AU - Jenkinson, Eric

AU - Anderson, Graham

AU - Lane, Peter

PY - 2007/2/15

Y1 - 2007/2/15

N2 - Lymphocytes from lymphotoxin (LT) alpha-deficient mice, which lack segregation of their B- and T-cell areas, acquire normal organization following adoptive transfer into RAG-deficient recipients, identifying a non-B non-T cell in the segregation process. Here we show that a CD4+CD3- accessory cell is tightly associated with discrete VCAM-1-expressing stromal cells in B- and T-cell areas of the mouse spleen. CD4+CD3- cells express high levels of LTalpha, LTbeta, and tumor necrosis factor (TNF) alpha, which are the ligands for the LTbeta receptor and TNFR1 expressed by stromal cells. The expression of these ligands is functional, as transferring CD4+CD3- cells derived from either embryonic or adult tissues into LTalpha-deficient mice organizes B/T segregation and up-regulates CCL21 protein expression in areas where T cells are segregated from B cells. We propose that the function of CD4+CD3- cells is to form a link between primed CD4 T cells and the underlying stromal elements, creating distinct microenvironments in which they enable effector responses.

AB - Lymphocytes from lymphotoxin (LT) alpha-deficient mice, which lack segregation of their B- and T-cell areas, acquire normal organization following adoptive transfer into RAG-deficient recipients, identifying a non-B non-T cell in the segregation process. Here we show that a CD4+CD3- accessory cell is tightly associated with discrete VCAM-1-expressing stromal cells in B- and T-cell areas of the mouse spleen. CD4+CD3- cells express high levels of LTalpha, LTbeta, and tumor necrosis factor (TNF) alpha, which are the ligands for the LTbeta receptor and TNFR1 expressed by stromal cells. The expression of these ligands is functional, as transferring CD4+CD3- cells derived from either embryonic or adult tissues into LTalpha-deficient mice organizes B/T segregation and up-regulates CCL21 protein expression in areas where T cells are segregated from B cells. We propose that the function of CD4+CD3- cells is to form a link between primed CD4 T cells and the underlying stromal elements, creating distinct microenvironments in which they enable effector responses.

UR - http://www.scopus.com/inward/record.url?scp=33846904226&partnerID=8YFLogxK

U2 - 10.1182/blood-2006-04-018465

DO - 10.1182/blood-2006-04-018465

M3 - Article

C2 - 17018858

VL - 109

SP - 1602

EP - 1610

JO - Blood

JF - Blood

SN - 0006-4971

IS - 4

ER -