Förster resonance energy transfer nanoplatform based on recognition-induced fusion/fission of DNA mixed micelles for nucleic acid sensing

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@article{4915182c1398497693f4a618b471656c,
title = "F{\"o}rster resonance energy transfer nanoplatform based on recognition-induced fusion/fission of DNA mixed micelles for nucleic acid sensing",
abstract = "The dynamic nature of micellar nanostructures is employed to form a self-assembled F{\"o}rster resonance energy transfer (FRET) nanoplatform for enhanced sensing of DNA. The platform consists of lipid oligonucleotide FRET probes incorporated into micellar scaffolds, where single recognition events result in fusion and fission of DNA mixed micelles, triggering the fluorescence response of multiple rather than a single FRET pair. In comparison to conventional FRET substrates where a single donor interacts with a single acceptor, the micellar multiplex FRET system showed ∼20- and ∼3-fold enhancements in the limit of detection and FRET efficiency, respectively. This supramolecular signal amplification approach could potentially be used to improve FRET-based diagnostic assays of nucleic acid and non-DNA based targets.",
keywords = "DNA sensing, FRET, lipid oligonucleotide conjugates, micelle, signal enhancement, DNA, Micelles, Fluorescence Resonance Energy Transfer, Nanostructures, Nucleic Acids, Lipid Oligonucleotide Conjugates, Dna Sensing, Micelle, Signal Enhancement",
author = "Setareh Vafaei and Francia Allabush and Tabaei, {Seyed R} and Louise Male and Dafforn, {Timothy R} and Tucker, {James H R} and Mendes, {Paula M}",
note = "Funding Information: We acknowledge financial support of this work by the EPSRC (Grant EP/K027263/1) and ERC (Consolidator Grant 614787). This project was also supported by the European Union{\textquoteright}s Horizon 2020 research and innovation program under the Marie Sk{\l}odowska-Curie Grant Agreement No. 795415. The Centre for Chemical and Materials Analysis at the University of Birmingham is acknowledged for technical support.",
year = "2021",
month = may,
day = "25",
doi = "10.1021/acsnano.1c00156",
language = "English",
volume = "15",
pages = "8517--8524",
journal = "ACS Nano",
issn = "1936-0851",
publisher = "American Chemical Society",
number = "5",

}

RIS

TY - JOUR

T1 - Förster resonance energy transfer nanoplatform based on recognition-induced fusion/fission of DNA mixed micelles for nucleic acid sensing

AU - Vafaei, Setareh

AU - Allabush, Francia

AU - Tabaei, Seyed R

AU - Male, Louise

AU - Dafforn, Timothy R

AU - Tucker, James H R

AU - Mendes, Paula M

N1 - Funding Information: We acknowledge financial support of this work by the EPSRC (Grant EP/K027263/1) and ERC (Consolidator Grant 614787). This project was also supported by the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie Grant Agreement No. 795415. The Centre for Chemical and Materials Analysis at the University of Birmingham is acknowledged for technical support.

PY - 2021/5/25

Y1 - 2021/5/25

N2 - The dynamic nature of micellar nanostructures is employed to form a self-assembled Förster resonance energy transfer (FRET) nanoplatform for enhanced sensing of DNA. The platform consists of lipid oligonucleotide FRET probes incorporated into micellar scaffolds, where single recognition events result in fusion and fission of DNA mixed micelles, triggering the fluorescence response of multiple rather than a single FRET pair. In comparison to conventional FRET substrates where a single donor interacts with a single acceptor, the micellar multiplex FRET system showed ∼20- and ∼3-fold enhancements in the limit of detection and FRET efficiency, respectively. This supramolecular signal amplification approach could potentially be used to improve FRET-based diagnostic assays of nucleic acid and non-DNA based targets.

AB - The dynamic nature of micellar nanostructures is employed to form a self-assembled Förster resonance energy transfer (FRET) nanoplatform for enhanced sensing of DNA. The platform consists of lipid oligonucleotide FRET probes incorporated into micellar scaffolds, where single recognition events result in fusion and fission of DNA mixed micelles, triggering the fluorescence response of multiple rather than a single FRET pair. In comparison to conventional FRET substrates where a single donor interacts with a single acceptor, the micellar multiplex FRET system showed ∼20- and ∼3-fold enhancements in the limit of detection and FRET efficiency, respectively. This supramolecular signal amplification approach could potentially be used to improve FRET-based diagnostic assays of nucleic acid and non-DNA based targets.

KW - DNA sensing

KW - FRET

KW - lipid oligonucleotide conjugates

KW - micelle

KW - signal enhancement

KW - DNA

KW - Micelles

KW - Fluorescence Resonance Energy Transfer

KW - Nanostructures

KW - Nucleic Acids

KW - Lipid Oligonucleotide Conjugates

KW - Dna Sensing

KW - Micelle

KW - Signal Enhancement

UR - http://www.scopus.com/inward/record.url?scp=85106368218&partnerID=8YFLogxK

U2 - 10.1021/acsnano.1c00156

DO - 10.1021/acsnano.1c00156

M3 - Article

C2 - 33961404

VL - 15

SP - 8517

EP - 8524

JO - ACS Nano

JF - ACS Nano

SN - 1936-0851

IS - 5

ER -