Fluoroquinolone resistance does not impose a cost on the fitness of Clostridium difficile in vitro

Research output: Contribution to journalArticlepeer-review


  • François Wasels
  • Stephen T Cartman
  • Patrizia Spigaglia
  • Fabrizio Barbanti
  • Nigel P Minton
  • Paola Mastrantonio

Colleges, School and Institutes


Point mutations conferring resistance to fluoroquinolones were introduced in the gyr genes of the reference strain Clostridium difficile 630. Only mutants with the substitution Thr-82→Ile in GyrA, which characterizes the hypervirulent epidemic clone III/027/NAP1, were resistant to all fluoroquinolones tested. The absence of a fitness cost in vitro for the most frequent mutations detected in resistant clinical isolates suggests that resistance will be maintained even in the absence of antibiotic pressure.


Original languageEnglish
Pages (from-to)1794-1796
Number of pages3
JournalAntimicrobial Agents and Chemotherapy
Issue number3
Early online date22 Dec 2014
Publication statusPublished - Mar 2015


  • Anti-Bacterial Agents, Clostridium difficile, DNA Gyrase, Drug Resistance, Bacterial, Fluoroquinolones, Point Mutation, Journal Article, Research Support, Non-U.S. Gov't