Fluorescent PSC-derived cardiomyocyte reporter lines: generation approaches and their applications in cardiovascular medicine

Research output: Contribution to journalReview articlepeer-review

Authors

Colleges, School and Institutes

External organisations

  • Wellcome Trust Centre for Human Genetics
  • University of Oxford

Abstract

Recent advances have made pluripotent stem cell (PSC)-derived cardiomyocytes an attractive option to model both normal and diseased cardiac function at the single-cell level. However, in vitro differentiation yields heterogeneous populations of cardiomyocytes and other cell types, potentially confounding phenotypic analyses. Fluorescent PSC-derived cardiomyocyte reporter systems allow specific cell lineages to be labelled, facilitating cell isolation for downstream applications including drug testing, disease modelling and cardiac regeneration. In this review, the different genetic strategies used to generate such reporter lines are presented with an emphasis on their relative technical advantages and disadvantages. Next, we explore how the fluorescent reporter lines have provided insights into cardiac development and cardiomyocyte physiology. Finally, we discuss how exciting new approaches using PSC-derived cardiomyocyte reporter lines are contributing to progress in cardiac cell therapy with respect to both graft adaptation and clinical safety.

Details

Original languageEnglish
Article number402
Number of pages26
JournalBiology
Volume9
Issue number11
Publication statusPublished - 16 Nov 2020

Keywords

  • fluorescent PSC-CM reporter lines, transgenic methods, CM purification, cardiac development, optical electrophysiology