Fluorescence tomography characterization for sub-surface imaging with protoporphyrin IX

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Fluorescence tomography characterization for sub-surface imaging with protoporphyrin IX. / Kepshire, D; Davis, SC; Dehghani, Hamid; Paulsen, KD; Pogue, BW.

In: Optics Express, Vol. 16, No. 12, 09.06.2008, p. 8581-8593.

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Kepshire, D ; Davis, SC ; Dehghani, Hamid ; Paulsen, KD ; Pogue, BW. / Fluorescence tomography characterization for sub-surface imaging with protoporphyrin IX. In: Optics Express. 2008 ; Vol. 16, No. 12. pp. 8581-8593.

Bibtex

@article{16f5212179e34aef91a159962f7613b5,
title = "Fluorescence tomography characterization for sub-surface imaging with protoporphyrin IX",
abstract = "Optical imaging of fluorescent objects embedded in a tissue simulating medium was characterized using non-contact based approaches to fluorescence remittance imaging (FRI) and sub-surface fluorescence diffuse optical tomography (FDOT). Using Protoporphyrin IX as a fluorescent agent, experiments were performed on tissue phantoms comprised of typical in-vivo tumor to normal tissue contrast ratios, ranging from 3.5:1 up to 10:1. It was found that tomographic imaging was able to recover interior inclusions with high contrast relative to the background; however, simple planar fluorescence imaging provided a superior contrast to noise ratio. Overall, FRI performed optimally when the object was located on or close to the surface and, perhaps most importantly, FDOT was able to recover specific depth information about the location of embedded regions. The results indicate that an optimal system for localizing embedded fluorescent regions should combine fluorescence reflectance imaging for high sensitivity and sub-surface tomography for depth detection, thereby allowing more accurate localization in all three directions within the tissue.",
author = "D Kepshire and SC Davis and Hamid Dehghani and KD Paulsen and BW Pogue",
year = "2008",
month = jun,
day = "9",
doi = "10.1364/OE.16.008581",
language = "English",
volume = "16",
pages = "8581--8593",
journal = "Optics Express",
issn = "1094-4087",
publisher = "Optical Society of America",
number = "12",

}

RIS

TY - JOUR

T1 - Fluorescence tomography characterization for sub-surface imaging with protoporphyrin IX

AU - Kepshire, D

AU - Davis, SC

AU - Dehghani, Hamid

AU - Paulsen, KD

AU - Pogue, BW

PY - 2008/6/9

Y1 - 2008/6/9

N2 - Optical imaging of fluorescent objects embedded in a tissue simulating medium was characterized using non-contact based approaches to fluorescence remittance imaging (FRI) and sub-surface fluorescence diffuse optical tomography (FDOT). Using Protoporphyrin IX as a fluorescent agent, experiments were performed on tissue phantoms comprised of typical in-vivo tumor to normal tissue contrast ratios, ranging from 3.5:1 up to 10:1. It was found that tomographic imaging was able to recover interior inclusions with high contrast relative to the background; however, simple planar fluorescence imaging provided a superior contrast to noise ratio. Overall, FRI performed optimally when the object was located on or close to the surface and, perhaps most importantly, FDOT was able to recover specific depth information about the location of embedded regions. The results indicate that an optimal system for localizing embedded fluorescent regions should combine fluorescence reflectance imaging for high sensitivity and sub-surface tomography for depth detection, thereby allowing more accurate localization in all three directions within the tissue.

AB - Optical imaging of fluorescent objects embedded in a tissue simulating medium was characterized using non-contact based approaches to fluorescence remittance imaging (FRI) and sub-surface fluorescence diffuse optical tomography (FDOT). Using Protoporphyrin IX as a fluorescent agent, experiments were performed on tissue phantoms comprised of typical in-vivo tumor to normal tissue contrast ratios, ranging from 3.5:1 up to 10:1. It was found that tomographic imaging was able to recover interior inclusions with high contrast relative to the background; however, simple planar fluorescence imaging provided a superior contrast to noise ratio. Overall, FRI performed optimally when the object was located on or close to the surface and, perhaps most importantly, FDOT was able to recover specific depth information about the location of embedded regions. The results indicate that an optimal system for localizing embedded fluorescent regions should combine fluorescence reflectance imaging for high sensitivity and sub-surface tomography for depth detection, thereby allowing more accurate localization in all three directions within the tissue.

U2 - 10.1364/OE.16.008581

DO - 10.1364/OE.16.008581

M3 - Article

C2 - 18545571

VL - 16

SP - 8581

EP - 8593

JO - Optics Express

JF - Optics Express

SN - 1094-4087

IS - 12

ER -