Flow cytometric analysis of E. coli on agar plates: implications for recombinant protein production.

Research output: Contribution to journalArticlepeer-review

Authors

Colleges, School and Institutes

Abstract

Recombinant protein production in bacterial hosts is a commercially important process in the pharmaceutical industry. Optimisation of such processes is of critical importance for process productivity and reproducibility. Here, flow cytometry methods were developed to assess characteristics of bacteria during two process steps that are infrequently studied: agar plate culture and liquid culture set-up. During storage on agar plates, three discrete populations of varying green fluorescence intensity were observed along with a progressive shift of cells from the high green fluorescence population to an intermediate green fluorescence population, observed to be due formation of amyloid inclusion bodies. The dynamics of cellular fluorescence and scatter properties upon setup of liquid cultures were also assessed. These methods have the potential to improve the development of fermentation set-up, a currently little-understood area.

Details

Original languageEnglish
Pages (from-to)1485-1494
Number of pages10
JournalBiotechnology Letters
Volume36
Issue number7
Early online date21 Mar 2014
Publication statusPublished - 1 Jul 2014

Keywords

  • Congo Red, Flow Cytometry, green fluorescent protein, Inclusion Bodies, recombinant protein production