Fibrinogen-CD11b/CD18 interaction activities the NF-kappa B pathway and delays apoptosis in human neutrophils
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Colleges, School and Institutes
The regulation of neutrophil half-life by members of the coagulation cascade is critical for the resolution of the inflammatory response. We have demonstrated that soluble fibrinogen (sFbg) delays human neutrophil (PMN) apoptosis through a mechanism that involves CD11b interactions, and phosphorylation of focal adhesion kinase (FAK) and extracellular signal-regulated kinase 1/2 (ERK1/2). Since NF-kappaB is a key element in the regulation of apoptotic mechanisms in several immune cells, we investigated whether NF-kappaB is involved in the control of PMN survival by sFbg. We show that sFbg triggers inhibitor protein kappaB (IkappaB-alpha) degradation and NF-kappaB activation. Furthermore, pharmacological inhibition of NF-kappaB abrogates sFbg effects on apoptosis. In addition, specific inhibition of MAPK ERK1/2 significantly reduces NF-kappaB translocation by sFbg, suggesting a relationship between ERK1/2 and NF-kappaB activation. Similar results are obtained when granulocytic-differentiated HL-60 cells are treated with sFbg, making this model highly attractive for integrin-induced gene expression studies. It can be concluded that NF-kappaB participates in the prevention of apoptosis induced by sFbg with the participation of MAPK ERK1/2. These results shed light on the molecular mechanisms that control human granulocyte apoptosis, and suggest that NF-kappaB regulation may be of benefit for the resolution of the inflammatory response.
|Number of pages||10|
|Journal||European Journal of Immunology|
|Publication status||Published - 1 May 2003|
- NF-kappa B, integrin, apoptosis, fibrinogen, neutrophil