TY - JOUR
T1 - Female meiosis in wild-type Arabidopsis thaliana and in two meiotic mutants
AU - Armstrong, Susan
AU - Jones, Gareth
PY - 2001/2/26
Y1 - 2001/2/26
N2 - Female meiosis in Arabidopsis has been analysed cytogenetically using an adaptation of a technique previously applied to male meiosis. Meiotic progression was closely correlated with stages of floral development, including the length and morphology of the gynoecium. Meiosis in embryo sac mother cells (EMCs) occurs later in development than male meiosis, in gynoecia that range in size between 0.3 and 0.8 mm. The earliest stages in EMCs coincide with the second division to tetrad stages in pollen mother cells. However, the details of meiotic chromosome behaviour in EMCs correspond closely to the observations we have previously made in male meiosis. In addition, BrdU labelling coupled with an immunolocalisation detection system was used to mark the S phase in cells preceding their entry into prophase I. These techniques allow female meiotic stages of Arabidopsis to be analysed in detail, from the S-phase through to the tetrad stage, and are shown to be equally applicable to the analysis of female meiosis in meiotic mutants.
AB - Female meiosis in Arabidopsis has been analysed cytogenetically using an adaptation of a technique previously applied to male meiosis. Meiotic progression was closely correlated with stages of floral development, including the length and morphology of the gynoecium. Meiosis in embryo sac mother cells (EMCs) occurs later in development than male meiosis, in gynoecia that range in size between 0.3 and 0.8 mm. The earliest stages in EMCs coincide with the second division to tetrad stages in pollen mother cells. However, the details of meiotic chromosome behaviour in EMCs correspond closely to the observations we have previously made in male meiosis. In addition, BrdU labelling coupled with an immunolocalisation detection system was used to mark the S phase in cells preceding their entry into prophase I. These techniques allow female meiotic stages of Arabidopsis to be analysed in detail, from the S-phase through to the tetrad stage, and are shown to be equally applicable to the analysis of female meiosis in meiotic mutants.
KW - meiosis
KW - megasporogenesis
KW - embryo sac mother cells
KW - Arabidopsis
UR - http://www.scopus.com/inward/record.url?scp=0034463356&partnerID=8YFLogxK
U2 - 10.1007/s004970000050
DO - 10.1007/s004970000050
M3 - Article
SN - 1432-2145
SN - 1432-2145
SN - 1432-2145
VL - 13
SP - 177
EP - 183
JO - Sexual Plant Reproduction
JF - Sexual Plant Reproduction
ER -