Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations

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Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations. / Gustafsson, Nils; Culley, Siân; Ashdown, George; Owen, Dylan M.; Pereira, Pedro Matos; Henriques, Ricardo.

In: Nature Communications, Vol. 7, 12471, 12.08.2016.

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Gustafsson, Nils ; Culley, Siân ; Ashdown, George ; Owen, Dylan M. ; Pereira, Pedro Matos ; Henriques, Ricardo. / Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations. In: Nature Communications. 2016 ; Vol. 7.

Bibtex

@article{174a2e8295004fa7b7fd2c20bc50a3af,
title = "Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations",
abstract = "Despite significant progress, high-speed live-cell super-resolution studies remain limited to specialized optical setups, generally requiring intense phototoxic illumination. Here, we describe a new analytical approach, super-resolution radial fluctuations (SRRF), provided as a fast graphics processing unit-enabled ImageJ plugin. In the most challenging data sets for super-resolution, such as those obtained in low-illumination live-cell imaging with GFP, we show that SRRF is generally capable of achieving resolutions better than 150 nm. Meanwhile, for data sets similar to those obtained in PALM or STORM imaging, SRRF achieves resolutions approaching those of standard single-molecule localization analysis. The broad applicability of SRRF and its performance at low signal-to-noise ratios allows super-resolution using modern widefield, confocal or TIRF microscopes with illumination orders of magnitude lower than methods such as PALM, STORM or STED. We demonstrate this by super-resolution live-cell imaging over timescales ranging from minutes to hours.",
author = "Nils Gustafsson and Si{\^a}n Culley and George Ashdown and Owen, {Dylan M.} and Pereira, {Pedro Matos} and Ricardo Henriques",
year = "2016",
month = aug,
day = "12",
doi = "10.1038/ncomms12471",
language = "English",
volume = "7",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Springer",

}

RIS

TY - JOUR

T1 - Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations

AU - Gustafsson, Nils

AU - Culley, Siân

AU - Ashdown, George

AU - Owen, Dylan M.

AU - Pereira, Pedro Matos

AU - Henriques, Ricardo

PY - 2016/8/12

Y1 - 2016/8/12

N2 - Despite significant progress, high-speed live-cell super-resolution studies remain limited to specialized optical setups, generally requiring intense phototoxic illumination. Here, we describe a new analytical approach, super-resolution radial fluctuations (SRRF), provided as a fast graphics processing unit-enabled ImageJ plugin. In the most challenging data sets for super-resolution, such as those obtained in low-illumination live-cell imaging with GFP, we show that SRRF is generally capable of achieving resolutions better than 150 nm. Meanwhile, for data sets similar to those obtained in PALM or STORM imaging, SRRF achieves resolutions approaching those of standard single-molecule localization analysis. The broad applicability of SRRF and its performance at low signal-to-noise ratios allows super-resolution using modern widefield, confocal or TIRF microscopes with illumination orders of magnitude lower than methods such as PALM, STORM or STED. We demonstrate this by super-resolution live-cell imaging over timescales ranging from minutes to hours.

AB - Despite significant progress, high-speed live-cell super-resolution studies remain limited to specialized optical setups, generally requiring intense phototoxic illumination. Here, we describe a new analytical approach, super-resolution radial fluctuations (SRRF), provided as a fast graphics processing unit-enabled ImageJ plugin. In the most challenging data sets for super-resolution, such as those obtained in low-illumination live-cell imaging with GFP, we show that SRRF is generally capable of achieving resolutions better than 150 nm. Meanwhile, for data sets similar to those obtained in PALM or STORM imaging, SRRF achieves resolutions approaching those of standard single-molecule localization analysis. The broad applicability of SRRF and its performance at low signal-to-noise ratios allows super-resolution using modern widefield, confocal or TIRF microscopes with illumination orders of magnitude lower than methods such as PALM, STORM or STED. We demonstrate this by super-resolution live-cell imaging over timescales ranging from minutes to hours.

U2 - 10.1038/ncomms12471

DO - 10.1038/ncomms12471

M3 - Article

VL - 7

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 12471

ER -