Expression of the lux genes in Streptococcus pneumoniae modulates pilus expression and virulence

Jenny A Herbert; Andrea M Mitchell; Ryan Ritchie; Jiangtao Ma; Kirsty Ross-Hutchinson; Timothy Mitchell

doi:10.1371/journal.pone.0189426

Expression of the lux genes in Streptococcus pneumoniae modulates pilus expression and virulence

Jenny A Herbert, Andrea M Mitchell, Ryan Ritchie, Jiangtao Ma, Kirsty Ross-Hutchinson, Timothy Mitchell

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Abstract

Bioluminescence has been harnessed for use in bacterial reporter systems and for in vivo imaging of infection in animal models. Strain Xen35, a bioluminescent derivative of Streptococcus pneumoniae serotype 4 strain TIGR4 was previously constructed for use for in vivo imaging of infections in animal models. We have shown that strain Xen35 is less virulent than its parent TIGR4 and that this is associated with the expression of the genes for bioluminescence. The expression of the luxA-E genes in the pneumococcus reduces virulence and down regulates the expression of the pneumococcal pilus.

Original language	English
Article number	e0189426
Journal	PLoS ONE
Volume	13
Issue number	1
DOIs	https://doi.org/10.1371/journal.pone.0189426
Publication status	Published - 17 Jan 2018

Keywords

Journal Article
Research Support, Non-U.S. Gov't

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abstract = "Bioluminescence has been harnessed for use in bacterial reporter systems and for in vivo imaging of infection in animal models. Strain Xen35, a bioluminescent derivative of Streptococcus pneumoniae serotype 4 strain TIGR4 was previously constructed for use for in vivo imaging of infections in animal models. We have shown that strain Xen35 is less virulent than its parent TIGR4 and that this is associated with the expression of the genes for bioluminescence. The expression of the luxA-E genes in the pneumococcus reduces virulence and down regulates the expression of the pneumococcal pilus.",

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AU - Herbert, Jenny A

AU - Mitchell, Andrea M

AU - Ritchie, Ryan

AU - Ma, Jiangtao

AU - Ross-Hutchinson, Kirsty

AU - Mitchell, Timothy

PY - 2018/1/17

Y1 - 2018/1/17

N2 - Bioluminescence has been harnessed for use in bacterial reporter systems and for in vivo imaging of infection in animal models. Strain Xen35, a bioluminescent derivative of Streptococcus pneumoniae serotype 4 strain TIGR4 was previously constructed for use for in vivo imaging of infections in animal models. We have shown that strain Xen35 is less virulent than its parent TIGR4 and that this is associated with the expression of the genes for bioluminescence. The expression of the luxA-E genes in the pneumococcus reduces virulence and down regulates the expression of the pneumococcal pilus.

AB - Bioluminescence has been harnessed for use in bacterial reporter systems and for in vivo imaging of infection in animal models. Strain Xen35, a bioluminescent derivative of Streptococcus pneumoniae serotype 4 strain TIGR4 was previously constructed for use for in vivo imaging of infections in animal models. We have shown that strain Xen35 is less virulent than its parent TIGR4 and that this is associated with the expression of the genes for bioluminescence. The expression of the luxA-E genes in the pneumococcus reduces virulence and down regulates the expression of the pneumococcal pilus.

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1371/journal.pone.0189426

DO - 10.1371/journal.pone.0189426

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SN - 1932-6203

VL - 13

JO - PLoS ONE

JF - PLoS ONE

IS - 1

M1 - e0189426

ER -

Expression of the lux genes in Streptococcus pneumoniae modulates pilus expression and virulence

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