Expression of the lux genes in Streptococcus pneumoniae modulates pilus expression and virulence

Research output: Contribution to journalArticle

Authors

  • Ryan Ritchie
  • Jiangtao Ma
  • Kirsty Ross-Hutchinson

External organisations

  • Institute of Microbiology and Infection, College of Medical and Dental Sciences, The University of Birmingham, Birmingham, United Kingdom.
  • University of Glasgow
  • Technology Hub Manager, Infrastructure and Facilities, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom.

Abstract

Bioluminescence has been harnessed for use in bacterial reporter systems and for in vivo imaging of infection in animal models. Strain Xen35, a bioluminescent derivative of Streptococcus pneumoniae serotype 4 strain TIGR4 was previously constructed for use for in vivo imaging of infections in animal models. We have shown that strain Xen35 is less virulent than its parent TIGR4 and that this is associated with the expression of the genes for bioluminescence. The expression of the luxA-E genes in the pneumococcus reduces virulence and down regulates the expression of the pneumococcal pilus.

Details

Original languageEnglish
Article numbere0189426
JournalPLoS ONE
Volume13
Issue number1
Publication statusPublished - 17 Jan 2018

Keywords

  • Journal Article, Research Support, Non-U.S. Gov't