Expression of RUNX1-ETO Rapidly Alters the Chromatin Landscape and Growth of Early Human Myeloid Precursor Cells
Research output: Contribution to journal › Article › peer-review
Authors
Colleges, School and Institutes
External organisations
- Melbourne Neuropsychiatry Centre, University of Melbourne and Melbourne Health
- Murdoch Children's Research Institute, Level 4 West, Royal Children's Hospital Melbourne
- Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, VIC, Australia.
Abstract
Acute myeloid leukemia (AML) is a hematopoietic malignancy caused by recurrent mutations in genes encoding transcriptional, chromatin, and/or signaling regulators. The t(8;21) translocation generates the aberrant transcription factor RUNX1-ETO (RUNX1-RUNX1T1), which by itself is insufficient to cause disease. t(8;21) AML patients show extensive chromatin reprogramming and have acquired additional mutations. Therefore, the genomic and developmental effects directly and solely attributable to RUNX1-ETO expression are unclear. To address this, we employ a human embryonic stem cell differentiation system capable of forming definitive myeloid progenitor cells to express RUNX1-ETO in an inducible fashion. Induction of RUNX1-ETO causes extensive chromatin reprogramming by interfering with RUNX1 binding, blocks differentiation, and arrests cellular growth, whereby growth arrest is reversible following RUNX1-ETO removal. Single-cell gene expression analyses show that RUNX1-ETO induction alters the differentiation of early myeloid progenitors, but not of other progenitor types, indicating that oncoprotein-mediated transcriptional reprogramming is highly target cell specific.
Bibliographic note
Details
Original language | English |
---|---|
Article number | 107691 |
Journal | Cell Reports |
Volume | 31 |
Issue number | 8 |
Publication status | Published - 26 May 2020 |
Keywords
- Acute Myeloid Leukemia (AML), RUNX1-ETO, chromatin, human ES cell differentiation, myelopoiesis, single cell RNA-Seq