Expression of 25-hydroxyvitamin D3-1alpha-hydroxylase in subcutaneous fat necrosis

Research output: Contribution to journalArticle

Authors

  • A Farooque
  • Celia Moss
  • D Zehnder
  • M Hewison
  • Nicholas Shaw

Colleges, School and Institutes

Abstract

BACKGROUND: The most serious complication of subcutaneous fat necrosis (SCFN), a rare condition of the newborn characterized by indurated purple nodules, is hypercalcaemia. However, the mechanism for this hypercalcaemia remains unclear. OBJECTIVES: To determine whether the hypercalcaemia associated with SCFN involves expression of the vitamin D-activating enzyme 25-hydroxyvitamin D(3)-1alpha-hydroxylase (1alpha-hydroxylase) in affected tissue. METHODS: Skin biopsies from two male patients with SCFN and hypercalcaemia were taken. The histological specimens were assessed using a polyclonal antibody against 1alpha-hydroxylase. RESULTS: Histology in both cases showed strong expression of 1alpha-hydroxylase protein (brown staining) within the inflammatory infiltrate associated with SCFN. This was consistent with similar experiments in other granulomatous conditions. CONCLUSIONS: Hypercalcaemia in SCFN appears to be due to abundant levels of 1alpha-hydroxylase in immune infiltrates associated with tissue lesions. This is consistent with previous observations of extrarenal 1alpha-hydroxylase in skin from other granulomatous conditions such as sarcoidosis and slack skin disease.

Details

Original languageEnglish
Pages (from-to)423-5
Number of pages3
JournalBritish Journal of Dermatology
Volume160
Issue number2
Publication statusPublished - 1 Feb 2009