Expression and regulation of the Escherichia coli O157:H7 effector proteins NleH1 and NleH2

Ashleigh Holmes; Cecilia S Lindestam Arlehamn; Dai Wang; Tim J Mitchell; Tom J Evans; Andrew J Roe

doi:10.1371/journal.pone.0033408

Expression and regulation of the Escherichia coli O157:H7 effector proteins NleH1 and NleH2

Ashleigh Holmes, Cecilia S Lindestam Arlehamn, Dai Wang, Tim J Mitchell, Tom J Evans, Andrew J Roe

Immunology and Immunotherapy

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

E. coli O157 carries two genes encoding the effector proteins NleH1 and NleH2 which are 87% identical. Despite the similarity between the proteins, the promoter regions upstream of the genes encoding the effectors are more divergent suggesting that the actual expression of the genes may be differentially regulated. This was tested by creating reporter fusions and examining their expression in different genetic backgrounds, media and on contact with host cells. The function of the proteins was also tested following transfection into host cells.

Original language	English
Pages (from-to)	e33408
Journal	PLoS ONE
Volume	7
Issue number	3
DOIs	https://doi.org/10.1371/journal.pone.0033408
Publication status	Published - 2012

Keywords

Bacterial Secretion Systems
DNA Primers
Escherichia coli O157
Escherichia coli Proteins
Gene Expression Regulation, Bacterial
HEK293 Cells
Humans
Luciferases
Microscopy, Fluorescence
Promoter Regions, Genetic
Real-Time Polymerase Chain Reaction
Trans-Activators

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abstract = "E. coli O157 carries two genes encoding the effector proteins NleH1 and NleH2 which are 87% identical. Despite the similarity between the proteins, the promoter regions upstream of the genes encoding the effectors are more divergent suggesting that the actual expression of the genes may be differentially regulated. This was tested by creating reporter fusions and examining their expression in different genetic backgrounds, media and on contact with host cells. The function of the proteins was also tested following transfection into host cells.",

keywords = "Bacterial Secretion Systems, DNA Primers, Escherichia coli O157, Escherichia coli Proteins, Gene Expression Regulation, Bacterial, HEK293 Cells, Humans, Luciferases, Microscopy, Fluorescence, Promoter Regions, Genetic, Real-Time Polymerase Chain Reaction, Trans-Activators",

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AU - Holmes, Ashleigh

AU - Lindestam Arlehamn, Cecilia S

AU - Wang, Dai

AU - Mitchell, Tim J

AU - Evans, Tom J

AU - Roe, Andrew J

PY - 2012

Y1 - 2012

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AB - E. coli O157 carries two genes encoding the effector proteins NleH1 and NleH2 which are 87% identical. Despite the similarity between the proteins, the promoter regions upstream of the genes encoding the effectors are more divergent suggesting that the actual expression of the genes may be differentially regulated. This was tested by creating reporter fusions and examining their expression in different genetic backgrounds, media and on contact with host cells. The function of the proteins was also tested following transfection into host cells.

KW - Bacterial Secretion Systems

KW - DNA Primers

KW - Escherichia coli O157

KW - Escherichia coli Proteins

KW - Gene Expression Regulation, Bacterial

KW - HEK293 Cells

KW - Humans

KW - Luciferases

KW - Microscopy, Fluorescence

KW - Promoter Regions, Genetic

KW - Real-Time Polymerase Chain Reaction

KW - Trans-Activators

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DO - 10.1371/journal.pone.0033408

M3 - Article

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SN - 1932-6203

VL - 7

SP - e33408

JO - PLoS ONE

JF - PLoS ONE

IS - 3

ER -

Expression and regulation of the Escherichia coli O157:H7 effector proteins NleH1 and NleH2

Abstract

Keywords

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