Exploiting cell-mediated contraction and adhesion to structure tissues in vitro

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Exploiting cell-mediated contraction and adhesion to structure tissues in vitro. / Wudebwe, U. N. G.; Bannerman, A.; Goldberg-oppenheimer, P.; Paxton, J. Z.; Williams, Richard; Grover, L. M.

In: Royal Society of London. Proceedings B. Biological Sciences, Vol. 370, No. 1661, 20140200, 05.02.2015.

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@article{c3a3ec61ea934dc9af2d6536e3c765ca,
title = "Exploiting cell-mediated contraction and adhesion to structure tissues in vitro",
abstract = "Progress in tissue engineering is now impacting beyond the field of regenerative medicine. Engineered tissues are now used as tools to evaluate the toxicity of compounds or even to enable the modelling of disease. While many of the materials that are used to facilitate tissue growth are designed to enable cell attachment, many researchers consider that the contraction and modification of these matrices by attached cells is not desirable and take measures to prevent this from occurring. Where substantial alignment of the molecules within tissues, however, is a feature of structure the process of contraction can be exploited to guide new matrix deposition. In this paper, we will demonstrate how we have used the cell contraction process to generate tissues with high levels of organization. The tissues that have been grown in the laboratory have been characterized using a suite of analytical techniques to demonstrate significant levels of matrix organization and mechanical behaviour analogous to natural tissues. This paper provides an overview of research that has been undertaken to determine how tissues have been grown in vitro with structuring from the molecular, right through to the macroscopic level.",
keywords = "extracellular matrix, ligament, tendon, tissue engineering, collagen, ceramic",
author = "Wudebwe, {U. N. G.} and A. Bannerman and P. Goldberg-oppenheimer and Paxton, {J. Z.} and Richard Williams and Grover, {L. M.}",
year = "2015",
month = feb,
day = "5",
doi = "10.1098/rstb.2014.0200",
language = "English",
volume = "370",
journal = "Royal Society of London. Proceedings B. Biological Sciences",
issn = "0962-8452",
publisher = "The Royal Society",
number = "1661",

}

RIS

TY - JOUR

T1 - Exploiting cell-mediated contraction and adhesion to structure tissues in vitro

AU - Wudebwe, U. N. G.

AU - Bannerman, A.

AU - Goldberg-oppenheimer, P.

AU - Paxton, J. Z.

AU - Williams, Richard

AU - Grover, L. M.

PY - 2015/2/5

Y1 - 2015/2/5

N2 - Progress in tissue engineering is now impacting beyond the field of regenerative medicine. Engineered tissues are now used as tools to evaluate the toxicity of compounds or even to enable the modelling of disease. While many of the materials that are used to facilitate tissue growth are designed to enable cell attachment, many researchers consider that the contraction and modification of these matrices by attached cells is not desirable and take measures to prevent this from occurring. Where substantial alignment of the molecules within tissues, however, is a feature of structure the process of contraction can be exploited to guide new matrix deposition. In this paper, we will demonstrate how we have used the cell contraction process to generate tissues with high levels of organization. The tissues that have been grown in the laboratory have been characterized using a suite of analytical techniques to demonstrate significant levels of matrix organization and mechanical behaviour analogous to natural tissues. This paper provides an overview of research that has been undertaken to determine how tissues have been grown in vitro with structuring from the molecular, right through to the macroscopic level.

AB - Progress in tissue engineering is now impacting beyond the field of regenerative medicine. Engineered tissues are now used as tools to evaluate the toxicity of compounds or even to enable the modelling of disease. While many of the materials that are used to facilitate tissue growth are designed to enable cell attachment, many researchers consider that the contraction and modification of these matrices by attached cells is not desirable and take measures to prevent this from occurring. Where substantial alignment of the molecules within tissues, however, is a feature of structure the process of contraction can be exploited to guide new matrix deposition. In this paper, we will demonstrate how we have used the cell contraction process to generate tissues with high levels of organization. The tissues that have been grown in the laboratory have been characterized using a suite of analytical techniques to demonstrate significant levels of matrix organization and mechanical behaviour analogous to natural tissues. This paper provides an overview of research that has been undertaken to determine how tissues have been grown in vitro with structuring from the molecular, right through to the macroscopic level.

KW - extracellular matrix

KW - ligament

KW - tendon

KW - tissue engineering

KW - collagen

KW - ceramic

U2 - 10.1098/rstb.2014.0200

DO - 10.1098/rstb.2014.0200

M3 - Article

C2 - 25533106

VL - 370

JO - Royal Society of London. Proceedings B. Biological Sciences

JF - Royal Society of London. Proceedings B. Biological Sciences

SN - 0962-8452

IS - 1661

M1 - 20140200

ER -