Exploitation of the Escherichia coli lac operon promoter for controlled recombinant protein production

Research output: Contribution to journalReview articlepeer-review


Colleges, School and Institutes

External organisations

  • University of Birmingham
  • School of Biosciences, University of Kent, Ingram Building, Canterbury CT2 7NJ, U.K.


The Escherichia coli lac operon promoter is widely used as a tool to control recombinant protein production in bacteria. Here, we give a brief review of how it functions, how it is regulated, and how, based on this knowledge, a suite of lac promoter derivatives has been developed to give a controlled expression that is suitable for diverse biotechnology applications.

Bibliographic note

© 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.


Original languageEnglish
Pages (from-to)755-763
Number of pages9
JournalBiochemical Society Transactions
Issue number2
Early online date10 Apr 2019
Publication statusPublished - 30 Apr 2019


  • Escherichia coli/genetics, Lac Operon/genetics, Promoter Regions, Genetic/genetics, Recombinant Proteins/genetics, Repressor Proteins/genetics, Transcription, Genetic/genetics

ASJC Scopus subject areas