Evidence of intrinsic impairment of osteoblast phenotype at the curve apex in girls with adolescent idiopathic scoliosis

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Evidence of intrinsic impairment of osteoblast phenotype at the curve apex in girls with adolescent idiopathic scoliosis. / Pearson, Mark; Philip, Ashleigh M; Nicholson, Thomas; Cooke, Megan; Grover, Liam; Newton Ede, Matthew; Jones, Simon.

In: Spine Deformity, Vol. 7, No. 4, 01.07.2019, p. 533-542.

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@article{11ad08d81a5641e584b73b214a113160,
title = "Evidence of intrinsic impairment of osteoblast phenotype at the curve apex in girls with adolescent idiopathic scoliosis",
abstract = "Study Design: An observational descriptive study based on a single cohort of patients.Objective: To determine whether spinal facet osteoblasts at the curve apex display a different phenotype to osteoblasts from outside the curve in adolescent idiopathic scoliosis (AIS) patients.Summary of Background Data: Intrinsic differences in the phenotype of spinal facet bone tissue and in spinal osteoblasts have been implicated in the pathology of AIS. However, no study has compared the phenotype of facet osteoblasts at the curve apex compared with outside the curve in AIS patients.Methods: Facet spinal tissue was collected perioperatively from three sites, the concave and convex side at the curve apex and from outside the curve (noncurve) from three AIS female patients aged 13–16 years. Spinal tissue was analyzed by micro–computed tomography to determine bone mineral density (BMD) and trabecular structure. Primary osteoblasts were cultured from concave, convex, and noncurve facet bone chips. The phenotype of osteoblasts was determined by assessment of cellular proliferation, cellular metabolism (alkaline phosphatase and Seahorse Analyzer), bone nodule mineralization (Alizarin red assay), and the mRNA expression of Wnt signaling genes (quantitative reverse transcriptase polymerase chain reaction).Results: Convex facet tissue exhibited greater BMD and trabecular thickness, compared with concave facet tissue. Osteoblasts at the convex side of the curve apex exhibited a significantly higher proliferative and metabolic phenotype and a greater capacity to form mineralized bone nodules, compared with concave osteoblasts. mRNA expression of SKP2 was significantly greater in both concave and convex osteoblasts, compared with noncurve osteoblasts. The expression of SFRP1 was significantly downregulated in convex osteoblasts, compared with either concave or noncurve.Conclusions: Intrinsic differences that affect osteoblast function are exhibited by spinal facet osteoblasts at the curve apex in AIS patients.",
keywords = "Adolescent idiopathic scoliosis, Bone, Curve apex, Osteoblasts, Wnt signalling",
author = "Mark Pearson and Philip, {Ashleigh M} and Thomas Nicholson and Megan Cooke and Liam Grover and {Newton Ede}, Matthew and Simon Jones",
year = "2019",
month = jul,
day = "1",
doi = "10.1016/j.jspd.2018.11.016",
language = "English",
volume = "7",
pages = "533--542",
journal = "Spine Deformity",
issn = "2212-134X",
publisher = "Elsevier",
number = "4",

}

RIS

TY - JOUR

T1 - Evidence of intrinsic impairment of osteoblast phenotype at the curve apex in girls with adolescent idiopathic scoliosis

AU - Pearson, Mark

AU - Philip, Ashleigh M

AU - Nicholson, Thomas

AU - Cooke, Megan

AU - Grover, Liam

AU - Newton Ede, Matthew

AU - Jones, Simon

PY - 2019/7/1

Y1 - 2019/7/1

N2 - Study Design: An observational descriptive study based on a single cohort of patients.Objective: To determine whether spinal facet osteoblasts at the curve apex display a different phenotype to osteoblasts from outside the curve in adolescent idiopathic scoliosis (AIS) patients.Summary of Background Data: Intrinsic differences in the phenotype of spinal facet bone tissue and in spinal osteoblasts have been implicated in the pathology of AIS. However, no study has compared the phenotype of facet osteoblasts at the curve apex compared with outside the curve in AIS patients.Methods: Facet spinal tissue was collected perioperatively from three sites, the concave and convex side at the curve apex and from outside the curve (noncurve) from three AIS female patients aged 13–16 years. Spinal tissue was analyzed by micro–computed tomography to determine bone mineral density (BMD) and trabecular structure. Primary osteoblasts were cultured from concave, convex, and noncurve facet bone chips. The phenotype of osteoblasts was determined by assessment of cellular proliferation, cellular metabolism (alkaline phosphatase and Seahorse Analyzer), bone nodule mineralization (Alizarin red assay), and the mRNA expression of Wnt signaling genes (quantitative reverse transcriptase polymerase chain reaction).Results: Convex facet tissue exhibited greater BMD and trabecular thickness, compared with concave facet tissue. Osteoblasts at the convex side of the curve apex exhibited a significantly higher proliferative and metabolic phenotype and a greater capacity to form mineralized bone nodules, compared with concave osteoblasts. mRNA expression of SKP2 was significantly greater in both concave and convex osteoblasts, compared with noncurve osteoblasts. The expression of SFRP1 was significantly downregulated in convex osteoblasts, compared with either concave or noncurve.Conclusions: Intrinsic differences that affect osteoblast function are exhibited by spinal facet osteoblasts at the curve apex in AIS patients.

AB - Study Design: An observational descriptive study based on a single cohort of patients.Objective: To determine whether spinal facet osteoblasts at the curve apex display a different phenotype to osteoblasts from outside the curve in adolescent idiopathic scoliosis (AIS) patients.Summary of Background Data: Intrinsic differences in the phenotype of spinal facet bone tissue and in spinal osteoblasts have been implicated in the pathology of AIS. However, no study has compared the phenotype of facet osteoblasts at the curve apex compared with outside the curve in AIS patients.Methods: Facet spinal tissue was collected perioperatively from three sites, the concave and convex side at the curve apex and from outside the curve (noncurve) from three AIS female patients aged 13–16 years. Spinal tissue was analyzed by micro–computed tomography to determine bone mineral density (BMD) and trabecular structure. Primary osteoblasts were cultured from concave, convex, and noncurve facet bone chips. The phenotype of osteoblasts was determined by assessment of cellular proliferation, cellular metabolism (alkaline phosphatase and Seahorse Analyzer), bone nodule mineralization (Alizarin red assay), and the mRNA expression of Wnt signaling genes (quantitative reverse transcriptase polymerase chain reaction).Results: Convex facet tissue exhibited greater BMD and trabecular thickness, compared with concave facet tissue. Osteoblasts at the convex side of the curve apex exhibited a significantly higher proliferative and metabolic phenotype and a greater capacity to form mineralized bone nodules, compared with concave osteoblasts. mRNA expression of SKP2 was significantly greater in both concave and convex osteoblasts, compared with noncurve osteoblasts. The expression of SFRP1 was significantly downregulated in convex osteoblasts, compared with either concave or noncurve.Conclusions: Intrinsic differences that affect osteoblast function are exhibited by spinal facet osteoblasts at the curve apex in AIS patients.

KW - Adolescent idiopathic scoliosis

KW - Bone

KW - Curve apex

KW - Osteoblasts

KW - Wnt signalling

UR - http://www.scopus.com/inward/record.url?scp=85059749533&partnerID=8YFLogxK

U2 - 10.1016/j.jspd.2018.11.016

DO - 10.1016/j.jspd.2018.11.016

M3 - Article

C2 - 31202368

VL - 7

SP - 533

EP - 542

JO - Spine Deformity

JF - Spine Deformity

SN - 2212-134X

IS - 4

ER -