Evaluation of PCR in the molecular diagnosis of endocarditis

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Evaluation of PCR in the molecular diagnosis of endocarditis. / Lang, S; Watkin, Richard; Lambert, PA; Bonser, Robert; Littler, William; Elliott, Thomas.

In: Journal of Infection, Vol. 48, No. 3, 01.04.2004, p. 269-275.

Research output: Contribution to journalArticle

Harvard

Lang, S, Watkin, R, Lambert, PA, Bonser, R, Littler, W & Elliott, T 2004, 'Evaluation of PCR in the molecular diagnosis of endocarditis', Journal of Infection, vol. 48, no. 3, pp. 269-275. https://doi.org/10.1016/S0163-4453(03)00102-6

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Vancouver

Author

Lang, S ; Watkin, Richard ; Lambert, PA ; Bonser, Robert ; Littler, William ; Elliott, Thomas. / Evaluation of PCR in the molecular diagnosis of endocarditis. In: Journal of Infection. 2004 ; Vol. 48, No. 3. pp. 269-275.

Bibtex

@article{b717d838c7e644309ecace6012e58c6c,
title = "Evaluation of PCR in the molecular diagnosis of endocarditis",
abstract = "OBJECTIVE: Infective endocarditis (IE) is diagnosed by the Duke criteria, which can be inconclusive particularly when blood cultures are negative. This study investigated the application of polymerase chain reaction (PCR) to identify bacterial DNA in excised valvular tissue, and its role in establishing the diagnosis of IE. METHODS: Ninety-eight patients undergoing valve replacement surgery were studied. Twenty-eight patients were confirmed as definite for endocarditis by the Duke criteria; nine were considered as possible and 61 had no known or previous microbial infection of the endocardium. A broad-range PCR technique was used to amplify prokaryotic 16S rRNA genes present within homogenised heart valve tissue. Subsequent DNA sequencing of the PCR amplicon allowed identification of the infecting microorganism. RESULTS: PCR results demonstrated the presence of bacterial DNA in the heart valves obtained from 14 out of 20 (70%) definite IE patients with positive blood cultures preoperatively. The causative microorganism for one patient with definite culture negative endocarditis was identified by PCR. Two out of nine (22%) of the valves from possible endocarditis patients also had bacterial DNA present converting them into the definite criteria whereas in the valves of seven out of nine (78%) of these patients no bacterial DNA was detected. CONCLUSION: The application of PCR to the explanted valves in patients with possible or confirmed diagnosis can augment the Duke criteria thereby improving post-surgical antimicrobial therapeutic options.",
author = "S Lang and Richard Watkin and PA Lambert and Robert Bonser and William Littler and Thomas Elliott",
year = "2004",
month = apr,
day = "1",
doi = "10.1016/S0163-4453(03)00102-6",
language = "English",
volume = "48",
pages = "269--275",
journal = "Journal of Infection",
issn = "0163-4453",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - Evaluation of PCR in the molecular diagnosis of endocarditis

AU - Lang, S

AU - Watkin, Richard

AU - Lambert, PA

AU - Bonser, Robert

AU - Littler, William

AU - Elliott, Thomas

PY - 2004/4/1

Y1 - 2004/4/1

N2 - OBJECTIVE: Infective endocarditis (IE) is diagnosed by the Duke criteria, which can be inconclusive particularly when blood cultures are negative. This study investigated the application of polymerase chain reaction (PCR) to identify bacterial DNA in excised valvular tissue, and its role in establishing the diagnosis of IE. METHODS: Ninety-eight patients undergoing valve replacement surgery were studied. Twenty-eight patients were confirmed as definite for endocarditis by the Duke criteria; nine were considered as possible and 61 had no known or previous microbial infection of the endocardium. A broad-range PCR technique was used to amplify prokaryotic 16S rRNA genes present within homogenised heart valve tissue. Subsequent DNA sequencing of the PCR amplicon allowed identification of the infecting microorganism. RESULTS: PCR results demonstrated the presence of bacterial DNA in the heart valves obtained from 14 out of 20 (70%) definite IE patients with positive blood cultures preoperatively. The causative microorganism for one patient with definite culture negative endocarditis was identified by PCR. Two out of nine (22%) of the valves from possible endocarditis patients also had bacterial DNA present converting them into the definite criteria whereas in the valves of seven out of nine (78%) of these patients no bacterial DNA was detected. CONCLUSION: The application of PCR to the explanted valves in patients with possible or confirmed diagnosis can augment the Duke criteria thereby improving post-surgical antimicrobial therapeutic options.

AB - OBJECTIVE: Infective endocarditis (IE) is diagnosed by the Duke criteria, which can be inconclusive particularly when blood cultures are negative. This study investigated the application of polymerase chain reaction (PCR) to identify bacterial DNA in excised valvular tissue, and its role in establishing the diagnosis of IE. METHODS: Ninety-eight patients undergoing valve replacement surgery were studied. Twenty-eight patients were confirmed as definite for endocarditis by the Duke criteria; nine were considered as possible and 61 had no known or previous microbial infection of the endocardium. A broad-range PCR technique was used to amplify prokaryotic 16S rRNA genes present within homogenised heart valve tissue. Subsequent DNA sequencing of the PCR amplicon allowed identification of the infecting microorganism. RESULTS: PCR results demonstrated the presence of bacterial DNA in the heart valves obtained from 14 out of 20 (70%) definite IE patients with positive blood cultures preoperatively. The causative microorganism for one patient with definite culture negative endocarditis was identified by PCR. Two out of nine (22%) of the valves from possible endocarditis patients also had bacterial DNA present converting them into the definite criteria whereas in the valves of seven out of nine (78%) of these patients no bacterial DNA was detected. CONCLUSION: The application of PCR to the explanted valves in patients with possible or confirmed diagnosis can augment the Duke criteria thereby improving post-surgical antimicrobial therapeutic options.

UR - http://www.scopus.com/inward/record.url?scp=1642272244&partnerID=8YFLogxK

U2 - 10.1016/S0163-4453(03)00102-6

DO - 10.1016/S0163-4453(03)00102-6

M3 - Article

C2 - 15001306

VL - 48

SP - 269

EP - 275

JO - Journal of Infection

JF - Journal of Infection

SN - 0163-4453

IS - 3

ER -