Evaluating newly formulated osteoprotegerin-chitosan gel: an in vitro and in vivo study

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Evaluating newly formulated osteoprotegerin-chitosan gel : an in vitro and in vivo study. / Jayash, Soher; Hashim, Najihah Mohd; Misran, Misni; Baharuddin, Nor Adinar.

In: Research & Reviews: Journal of Material Sciences, Vol. 5, No. 4, 07.2017, p. 90.

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Jayash, Soher ; Hashim, Najihah Mohd ; Misran, Misni ; Baharuddin, Nor Adinar. / Evaluating newly formulated osteoprotegerin-chitosan gel : an in vitro and in vivo study. In: Research & Reviews: Journal of Material Sciences. 2017 ; Vol. 5, No. 4. pp. 90.

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@article{74c0d0bcf3534a93a7cc2b8033e36d8e,
title = "Evaluating newly formulated osteoprotegerin-chitosan gel: an in vitro and in vivo study",
abstract = "The binding of the receptor activator of nuclear factor kappa-B ligand (RANKL) to the receptor activator of nuclear factor kappa-B (RANK) on pre-osteoclast is essential for osteoclast maturation and activity. Osteoproteogerin (OPG) is a soluble decoy receptor for RANKL. Its binding to RANK prevents binding of RANKL to RANK and subsequent hinders activation of osteoclast. Various in vivo studies and clinical trials have investigated the systemic use of OPG in treatment of bone diseases. Chitosan is a cationic polymer derived from chitin. This material has antimicrobial activity, biodegradability, and mucoadhesive properties, making it an ideal material for biomedical applications. It also promotes cell adhesion, proliferation and differentiation. It is suggested that the local application of new formulated OPG-chitosan preparation may have similar osteogenic potential in the bone defect. The objectives of this study are: (i) to formulate of different forms of OPG-chitosan gels from different molecular weights of water-soluble chitosan (10, 25, 50 kDa), (ii) to evaluate the gels biodegradation, (iii) to determine amount of OPG protein release from gels and (iv) to evaluate the cytotoxicity of gels by Alamar Blue assay and scanning electron microscope. (iv) Efficacy of gel on bone regeneration in rabbit. From our studies we conclude that our gels are (i) non-toxic (ii) biodegradable and (iii) exhibits sustained release property. (iv) OPG-chitosan has a positive effect on bone formation. Therefore, we have proven that OPG-chitosan gel is evidently viable to be used locally for potential bone defect application.",
author = "Soher Jayash and Hashim, {Najihah Mohd} and Misni Misran and Baharuddin, {Nor Adinar}",
year = "2017",
month = jul,
language = "English",
volume = "5",
pages = "90",
journal = "Research & Reviews: Journal of Material Sciences",
issn = "2321-6212",
number = "4",
note = "10th International Conference on Emerging Materials and Nanotechnology ; Conference date: 27-07-2017 Through 29-07-2017",

}

RIS

TY - JOUR

T1 - Evaluating newly formulated osteoprotegerin-chitosan gel

T2 - 10th International Conference on Emerging Materials and Nanotechnology

AU - Jayash, Soher

AU - Hashim, Najihah Mohd

AU - Misran, Misni

AU - Baharuddin, Nor Adinar

PY - 2017/7

Y1 - 2017/7

N2 - The binding of the receptor activator of nuclear factor kappa-B ligand (RANKL) to the receptor activator of nuclear factor kappa-B (RANK) on pre-osteoclast is essential for osteoclast maturation and activity. Osteoproteogerin (OPG) is a soluble decoy receptor for RANKL. Its binding to RANK prevents binding of RANKL to RANK and subsequent hinders activation of osteoclast. Various in vivo studies and clinical trials have investigated the systemic use of OPG in treatment of bone diseases. Chitosan is a cationic polymer derived from chitin. This material has antimicrobial activity, biodegradability, and mucoadhesive properties, making it an ideal material for biomedical applications. It also promotes cell adhesion, proliferation and differentiation. It is suggested that the local application of new formulated OPG-chitosan preparation may have similar osteogenic potential in the bone defect. The objectives of this study are: (i) to formulate of different forms of OPG-chitosan gels from different molecular weights of water-soluble chitosan (10, 25, 50 kDa), (ii) to evaluate the gels biodegradation, (iii) to determine amount of OPG protein release from gels and (iv) to evaluate the cytotoxicity of gels by Alamar Blue assay and scanning electron microscope. (iv) Efficacy of gel on bone regeneration in rabbit. From our studies we conclude that our gels are (i) non-toxic (ii) biodegradable and (iii) exhibits sustained release property. (iv) OPG-chitosan has a positive effect on bone formation. Therefore, we have proven that OPG-chitosan gel is evidently viable to be used locally for potential bone defect application.

AB - The binding of the receptor activator of nuclear factor kappa-B ligand (RANKL) to the receptor activator of nuclear factor kappa-B (RANK) on pre-osteoclast is essential for osteoclast maturation and activity. Osteoproteogerin (OPG) is a soluble decoy receptor for RANKL. Its binding to RANK prevents binding of RANKL to RANK and subsequent hinders activation of osteoclast. Various in vivo studies and clinical trials have investigated the systemic use of OPG in treatment of bone diseases. Chitosan is a cationic polymer derived from chitin. This material has antimicrobial activity, biodegradability, and mucoadhesive properties, making it an ideal material for biomedical applications. It also promotes cell adhesion, proliferation and differentiation. It is suggested that the local application of new formulated OPG-chitosan preparation may have similar osteogenic potential in the bone defect. The objectives of this study are: (i) to formulate of different forms of OPG-chitosan gels from different molecular weights of water-soluble chitosan (10, 25, 50 kDa), (ii) to evaluate the gels biodegradation, (iii) to determine amount of OPG protein release from gels and (iv) to evaluate the cytotoxicity of gels by Alamar Blue assay and scanning electron microscope. (iv) Efficacy of gel on bone regeneration in rabbit. From our studies we conclude that our gels are (i) non-toxic (ii) biodegradable and (iii) exhibits sustained release property. (iv) OPG-chitosan has a positive effect on bone formation. Therefore, we have proven that OPG-chitosan gel is evidently viable to be used locally for potential bone defect application.

UR - http://www.rroij.com/archive/joms-volume-5-issue-4-year-2017.html

UR - http://www.rroij.com/aimsandscope-material-sciences.php

UR - http://10.4172/2321-6212-C1-003

M3 - Abstract

VL - 5

SP - 90

JO - Research & Reviews: Journal of Material Sciences

JF - Research & Reviews: Journal of Material Sciences

SN - 2321-6212

IS - 4

Y2 - 27 July 2017 through 29 July 2017

ER -