Estrogen sensitive liver transgenic zebrafish (Danio rerio) line (Tg(vtg1:mCherry)) suitable for the direct detection of estrogenicity in environmental samples

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Estrogen sensitive liver transgenic zebrafish (Danio rerio) line (Tg(vtg1:mCherry)) suitable for the direct detection of estrogenicity in environmental samples. / Bakos, Katalin; Kovacs, Robert; Balogh, Erna; Sipos, Dora Kanaine; Reining, Marta; Gyomorei-Neuberger, Orsolya; Balazs, Adrienn; Kriszt, Balazs; Bencsik, Dora; Csepeli, Andrea; Gazsi, Gyongyi; Hadzhiev, Yavor; Urbanyi, Bela; Mueller, Ferenc; Kovacs, Balazs; Csenki, Zsolt.

In: Aquatic Toxicology, Vol. 208, 01.03.2019, p. 157-167.

Research output: Contribution to journalArticle

Harvard

Bakos, K, Kovacs, R, Balogh, E, Sipos, DK, Reining, M, Gyomorei-Neuberger, O, Balazs, A, Kriszt, B, Bencsik, D, Csepeli, A, Gazsi, G, Hadzhiev, Y, Urbanyi, B, Mueller, F, Kovacs, B & Csenki, Z 2019, 'Estrogen sensitive liver transgenic zebrafish (Danio rerio) line (Tg(vtg1:mCherry)) suitable for the direct detection of estrogenicity in environmental samples', Aquatic Toxicology, vol. 208, pp. 157-167. https://doi.org/10.1016/j.aquatox.2019.01.008

APA

Bakos, K., Kovacs, R., Balogh, E., Sipos, D. K., Reining, M., Gyomorei-Neuberger, O., Balazs, A., Kriszt, B., Bencsik, D., Csepeli, A., Gazsi, G., Hadzhiev, Y., Urbanyi, B., Mueller, F., Kovacs, B., & Csenki, Z. (2019). Estrogen sensitive liver transgenic zebrafish (Danio rerio) line (Tg(vtg1:mCherry)) suitable for the direct detection of estrogenicity in environmental samples. Aquatic Toxicology, 208, 157-167. https://doi.org/10.1016/j.aquatox.2019.01.008

Vancouver

Author

Bakos, Katalin ; Kovacs, Robert ; Balogh, Erna ; Sipos, Dora Kanaine ; Reining, Marta ; Gyomorei-Neuberger, Orsolya ; Balazs, Adrienn ; Kriszt, Balazs ; Bencsik, Dora ; Csepeli, Andrea ; Gazsi, Gyongyi ; Hadzhiev, Yavor ; Urbanyi, Bela ; Mueller, Ferenc ; Kovacs, Balazs ; Csenki, Zsolt. / Estrogen sensitive liver transgenic zebrafish (Danio rerio) line (Tg(vtg1:mCherry)) suitable for the direct detection of estrogenicity in environmental samples. In: Aquatic Toxicology. 2019 ; Vol. 208. pp. 157-167.

Bibtex

@article{4d762c0de2ce4dcaa5042edeee2c899b,
title = "Estrogen sensitive liver transgenic zebrafish (Danio rerio) line (Tg(vtg1:mCherry)) suitable for the direct detection of estrogenicity in environmental samples",
abstract = "Environmental estrogens are a serious concern worldwide due to their ubiquity and adverse ecotoxicological and health effects. Chemical structure of these substances is highly diverse, therefore estrogenicity cannot be predicted on the basis of molecular structure. Furthermore, estimation of estrogenicity of environmental samples based on chemical analytics of suspects is difficult given the complex interaction of chemicals and the impact on estrogenicity. The full estrogenic impact of an environmental sample can thus only be revealed by a series of sensitive in vitro and in vivo ecotoxicological tests. Herein we describe a vitellogenin reporter transgenic zebrafish line (Tg(vtg1:mCherry)) that enables the detection of estrogenicity in the environmentally relevant, low concentration ranges in embryonic tests that are in accordance with 3Rs and relevant animal welfare regulations. The transgene construct used for the development of Tg(vtg1:mCherry) carried a long (3.4 kbp) natural vitellogenin-1 promoter sequence with a high number of ERE sites. A test protocol was developed based on our finding that the endogenous vitellogenin and the reporter show similar spatial expression pattern and both endogenous and vitellogenin reporter is only produced in the left hepatic lobe of 5 dpf zebrafish embryos. Seven generations of Tg(vtg1:mCherry) have been established, and the estrogen responsiveness was tested with different estrogenic substances and wastewater samples. Embryos were exposed from 3 to 5 days post fertilization (dpf). Fluorescence in embryos could be detected upon treatment with 17-{\ss}-estradiol from a concentration of 100 ng/L, 17-α-ethynilestradiol from 1 ng/L, zearalenone from 100 ng/L and bisphenol-A from 1 mg/L. In the adult stage transgene activity appeared to be more sensitive to estrogen treatment, with detectable transgene activity from 5 ng/L 17-{\ss}-estradiol concentration. The transgenic line Tg(vtg1:mCherry) was also suitable for the direct measurement of estrogenicity in wastewater samples without sample extraction. The detection of estrogenic activity using the reporter line was confirmed by the bioluminescent yeast estrogen screen.",
keywords = "transgenic, vitellogenin, endocrine disrupting chemicals, in vivo, YES",
author = "Katalin Bakos and Robert Kovacs and Erna Balogh and Sipos, {Dora Kanaine} and Marta Reining and Orsolya Gyomorei-Neuberger and Adrienn Balazs and Balazs Kriszt and Dora Bencsik and Andrea Csepeli and Gyongyi Gazsi and Yavor Hadzhiev and Bela Urbanyi and Ferenc Mueller and Balazs Kovacs and Zsolt Csenki",
year = "2019",
month = mar,
day = "1",
doi = "10.1016/j.aquatox.2019.01.008",
language = "English",
volume = "208",
pages = "157--167",
journal = "Aquatic Toxicology",
issn = "0166-445X",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Estrogen sensitive liver transgenic zebrafish (Danio rerio) line (Tg(vtg1:mCherry)) suitable for the direct detection of estrogenicity in environmental samples

AU - Bakos, Katalin

AU - Kovacs, Robert

AU - Balogh, Erna

AU - Sipos, Dora Kanaine

AU - Reining, Marta

AU - Gyomorei-Neuberger, Orsolya

AU - Balazs, Adrienn

AU - Kriszt, Balazs

AU - Bencsik, Dora

AU - Csepeli, Andrea

AU - Gazsi, Gyongyi

AU - Hadzhiev, Yavor

AU - Urbanyi, Bela

AU - Mueller, Ferenc

AU - Kovacs, Balazs

AU - Csenki, Zsolt

PY - 2019/3/1

Y1 - 2019/3/1

N2 - Environmental estrogens are a serious concern worldwide due to their ubiquity and adverse ecotoxicological and health effects. Chemical structure of these substances is highly diverse, therefore estrogenicity cannot be predicted on the basis of molecular structure. Furthermore, estimation of estrogenicity of environmental samples based on chemical analytics of suspects is difficult given the complex interaction of chemicals and the impact on estrogenicity. The full estrogenic impact of an environmental sample can thus only be revealed by a series of sensitive in vitro and in vivo ecotoxicological tests. Herein we describe a vitellogenin reporter transgenic zebrafish line (Tg(vtg1:mCherry)) that enables the detection of estrogenicity in the environmentally relevant, low concentration ranges in embryonic tests that are in accordance with 3Rs and relevant animal welfare regulations. The transgene construct used for the development of Tg(vtg1:mCherry) carried a long (3.4 kbp) natural vitellogenin-1 promoter sequence with a high number of ERE sites. A test protocol was developed based on our finding that the endogenous vitellogenin and the reporter show similar spatial expression pattern and both endogenous and vitellogenin reporter is only produced in the left hepatic lobe of 5 dpf zebrafish embryos. Seven generations of Tg(vtg1:mCherry) have been established, and the estrogen responsiveness was tested with different estrogenic substances and wastewater samples. Embryos were exposed from 3 to 5 days post fertilization (dpf). Fluorescence in embryos could be detected upon treatment with 17-ß-estradiol from a concentration of 100 ng/L, 17-α-ethynilestradiol from 1 ng/L, zearalenone from 100 ng/L and bisphenol-A from 1 mg/L. In the adult stage transgene activity appeared to be more sensitive to estrogen treatment, with detectable transgene activity from 5 ng/L 17-ß-estradiol concentration. The transgenic line Tg(vtg1:mCherry) was also suitable for the direct measurement of estrogenicity in wastewater samples without sample extraction. The detection of estrogenic activity using the reporter line was confirmed by the bioluminescent yeast estrogen screen.

AB - Environmental estrogens are a serious concern worldwide due to their ubiquity and adverse ecotoxicological and health effects. Chemical structure of these substances is highly diverse, therefore estrogenicity cannot be predicted on the basis of molecular structure. Furthermore, estimation of estrogenicity of environmental samples based on chemical analytics of suspects is difficult given the complex interaction of chemicals and the impact on estrogenicity. The full estrogenic impact of an environmental sample can thus only be revealed by a series of sensitive in vitro and in vivo ecotoxicological tests. Herein we describe a vitellogenin reporter transgenic zebrafish line (Tg(vtg1:mCherry)) that enables the detection of estrogenicity in the environmentally relevant, low concentration ranges in embryonic tests that are in accordance with 3Rs and relevant animal welfare regulations. The transgene construct used for the development of Tg(vtg1:mCherry) carried a long (3.4 kbp) natural vitellogenin-1 promoter sequence with a high number of ERE sites. A test protocol was developed based on our finding that the endogenous vitellogenin and the reporter show similar spatial expression pattern and both endogenous and vitellogenin reporter is only produced in the left hepatic lobe of 5 dpf zebrafish embryos. Seven generations of Tg(vtg1:mCherry) have been established, and the estrogen responsiveness was tested with different estrogenic substances and wastewater samples. Embryos were exposed from 3 to 5 days post fertilization (dpf). Fluorescence in embryos could be detected upon treatment with 17-ß-estradiol from a concentration of 100 ng/L, 17-α-ethynilestradiol from 1 ng/L, zearalenone from 100 ng/L and bisphenol-A from 1 mg/L. In the adult stage transgene activity appeared to be more sensitive to estrogen treatment, with detectable transgene activity from 5 ng/L 17-ß-estradiol concentration. The transgenic line Tg(vtg1:mCherry) was also suitable for the direct measurement of estrogenicity in wastewater samples without sample extraction. The detection of estrogenic activity using the reporter line was confirmed by the bioluminescent yeast estrogen screen.

KW - transgenic

KW - vitellogenin

KW - endocrine disrupting chemicals

KW - in vivo

KW - YES

UR - http://www.scopus.com/inward/record.url?scp=85060211459&partnerID=8YFLogxK

U2 - 10.1016/j.aquatox.2019.01.008

DO - 10.1016/j.aquatox.2019.01.008

M3 - Article

C2 - 30677711

VL - 208

SP - 157

EP - 167

JO - Aquatic Toxicology

JF - Aquatic Toxicology

SN - 0166-445X

ER -