Estimation of disruption of animal cells by laminar shear stress

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Colleges, School and Institutes

External organisations

  • SERC Centre for Biochem. Engineering
  • University of Birmingham

Abstract

Using mechanical cell properties measured by micromanipulation, and a model of cell distortion in laminar flow fields, a method has been developed for predicting disruption of animal cells by laminar shear stresses. Predictions of the model were compared with measured losses of cell number and viability of TB/C3 murine hybridomas sheared in a cone and plate viscometer at shear rates up to 3950 s-1, and shear stresses up to 600 Nm-2, achieved by enhancement of viscosity with dextran. In all cases, the experimental results and predictions were within 30%. Such excellent agreement suggests it might be possible to use micromanipulation measurements of animal cell mechanical properties to predict cell damage in more complex flow fields, such as those in bioreactors.

Details

Original languageEnglish
Pages (from-to)1004-1010
Number of pages7
JournalBiotechnology and Bioengineering
Volume40
Issue number9
Publication statusPublished - Nov 1992

ASJC Scopus subject areas