Equine transcriptome quantification using human GeneChip arrays can be improved using genomic DNA hybridisation and probe selection

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Equine transcriptome quantification using human GeneChip arrays can be improved using genomic DNA hybridisation and probe selection. / Graham, Neil S; Clutterbuck, Abigail L; James, Nicholas; Lea, Richard G; Mobasheri, Ali; Broadley, Martin R; May, Sean T.

In: The Veterinary Journal , Vol. 186, No. 3, 2010, p. 323-7.

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Graham, Neil S ; Clutterbuck, Abigail L ; James, Nicholas ; Lea, Richard G ; Mobasheri, Ali ; Broadley, Martin R ; May, Sean T. / Equine transcriptome quantification using human GeneChip arrays can be improved using genomic DNA hybridisation and probe selection. In: The Veterinary Journal . 2010 ; Vol. 186, No. 3. pp. 323-7.

Bibtex

@article{912881cc01da4e30b959cdf7b618c296,
title = "Equine transcriptome quantification using human GeneChip arrays can be improved using genomic DNA hybridisation and probe selection",
abstract = "Affymetrix GeneChip arrays are a powerful tool for transcriptome profiling and have been applied to a wide range of species. A genomic DNA (gDNA)-based probe selection method has been developed which broadens the range of species to which GeneChips may be successfully applied. This study demonstrated that gDNA-based probe selection on the Affymetrix U133+2 GeneChip array can be used to study the equine transcriptome which, to date, has received only limited attention. More than 29,000 transcripts can be detected in equine brain and liver and in primary cultures of equine articular chondrocytes. Gene ontology analysis of differentially expressed genes revealed the presence of expected categories within each tissue. The level of gene expression could also be correlated with the phenotypes and specialised functions of each tissue. The results demonstrated that probe selection on a human chip can be successfully used to study the equine transcriptome.",
author = "Graham, {Neil S} and Clutterbuck, {Abigail L} and Nicholas James and Lea, {Richard G} and Ali Mobasheri and Broadley, {Martin R} and May, {Sean T}",
note = "Copyright {\textcopyright} 2009 Elsevier Ltd. All rights reserved.",
year = "2010",
doi = "10.1016/j.tvjl.2009.08.030",
language = "English",
volume = "186",
pages = "323--7",
journal = "The Veterinary Journal ",
issn = "1090-0233",
publisher = "Elsevier (Singapore) Pte Ltd",
number = "3",

}

RIS

TY - JOUR

T1 - Equine transcriptome quantification using human GeneChip arrays can be improved using genomic DNA hybridisation and probe selection

AU - Graham, Neil S

AU - Clutterbuck, Abigail L

AU - James, Nicholas

AU - Lea, Richard G

AU - Mobasheri, Ali

AU - Broadley, Martin R

AU - May, Sean T

N1 - Copyright © 2009 Elsevier Ltd. All rights reserved.

PY - 2010

Y1 - 2010

N2 - Affymetrix GeneChip arrays are a powerful tool for transcriptome profiling and have been applied to a wide range of species. A genomic DNA (gDNA)-based probe selection method has been developed which broadens the range of species to which GeneChips may be successfully applied. This study demonstrated that gDNA-based probe selection on the Affymetrix U133+2 GeneChip array can be used to study the equine transcriptome which, to date, has received only limited attention. More than 29,000 transcripts can be detected in equine brain and liver and in primary cultures of equine articular chondrocytes. Gene ontology analysis of differentially expressed genes revealed the presence of expected categories within each tissue. The level of gene expression could also be correlated with the phenotypes and specialised functions of each tissue. The results demonstrated that probe selection on a human chip can be successfully used to study the equine transcriptome.

AB - Affymetrix GeneChip arrays are a powerful tool for transcriptome profiling and have been applied to a wide range of species. A genomic DNA (gDNA)-based probe selection method has been developed which broadens the range of species to which GeneChips may be successfully applied. This study demonstrated that gDNA-based probe selection on the Affymetrix U133+2 GeneChip array can be used to study the equine transcriptome which, to date, has received only limited attention. More than 29,000 transcripts can be detected in equine brain and liver and in primary cultures of equine articular chondrocytes. Gene ontology analysis of differentially expressed genes revealed the presence of expected categories within each tissue. The level of gene expression could also be correlated with the phenotypes and specialised functions of each tissue. The results demonstrated that probe selection on a human chip can be successfully used to study the equine transcriptome.

U2 - 10.1016/j.tvjl.2009.08.030

DO - 10.1016/j.tvjl.2009.08.030

M3 - Article

C2 - 19786357

VL - 186

SP - 323

EP - 327

JO - The Veterinary Journal

JF - The Veterinary Journal

SN - 1090-0233

IS - 3

ER -