Engineering Microbial Physiology with Synthetic Polymers: Cationic Polymers Induce Biofilm Formation in Vibrio cholerae and Downregulate the Expression of Virulence Genes

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Engineering Microbial Physiology with Synthetic Polymers : Cationic Polymers Induce Biofilm Formation in Vibrio cholerae and Downregulate the Expression of Virulence Genes. / Perez Soto, Nicolas; Moule, Lauren; Crisan, Daniel; Insua Lopez, Ignacio; Taylor-Smith, Leanne; Voelz, Kerstin; Fernandez-Trillo, Francisco; Krachler, Anne Marie.

In: Chemical Science, 16.05.2017.

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@article{7cdf5d547ef341d397aeaab63691da05,
title = "Engineering Microbial Physiology with Synthetic Polymers: Cationic Polymers Induce Biofilm Formation in Vibrio cholerae and Downregulate the Expression of Virulence Genes",
abstract = "Here we report the first application of non-bactericidal synthetic polymers to modulate the physiology of a bacterial pathogen. Poly(N-[3- (dimethylamino)propyl] methacrylamide) (P1) and poly(N-(3-aminopropyl)methacrylamide) (P2), cationic polymers that bind to the surface of V. cholerae, the infectious agent causing cholera disease, can sequester the pathogen into clusters. Upon clustering, V. cholerae transitions to a sessile lifestyle, characterised by increased biofilm production and the repression of key virulence factors such as the cholera toxin (CTX). Moreover, clustering the pathogen results in the minimisation of adherence and toxicity to intestinal epithelial cells. Our results suggest that the reduction in toxicity is associated with the reduction to the number of free bacteria, but also the downregulation of toxin production. Finally we demonstrate that these polymers can reduce colonisation of zebrafish larvae upon ingestion of water contaminated with V. cholerae. Overall, our results suggest that the physiology of this pathogen can be modulated without the need to genetically manipulate the microorganism and that this modulation is an off-target effect that results from the intrinsic ability of the pathogen to sense and adapt to its environment. We believe these findings pave the way towards a better understanding of the interactions between pathogenic bacteria and polymeric materials and will underpin the development of novel antimicrobial polymers.",
author = "{Perez Soto}, Nicolas and Lauren Moule and Daniel Crisan and {Insua Lopez}, Ignacio and Leanne Taylor-Smith and Kerstin Voelz and Francisco Fernandez-Trillo and Krachler, {Anne Marie}",
year = "2017",
month = may,
day = "16",
doi = "10.1039/C7SC00615B",
language = "English",
journal = "Chemical Science",
issn = "2041-6520",
publisher = "Royal Society of Chemistry",

}

RIS

TY - JOUR

T1 - Engineering Microbial Physiology with Synthetic Polymers

T2 - Cationic Polymers Induce Biofilm Formation in Vibrio cholerae and Downregulate the Expression of Virulence Genes

AU - Perez Soto, Nicolas

AU - Moule, Lauren

AU - Crisan, Daniel

AU - Insua Lopez, Ignacio

AU - Taylor-Smith, Leanne

AU - Voelz, Kerstin

AU - Fernandez-Trillo, Francisco

AU - Krachler, Anne Marie

PY - 2017/5/16

Y1 - 2017/5/16

N2 - Here we report the first application of non-bactericidal synthetic polymers to modulate the physiology of a bacterial pathogen. Poly(N-[3- (dimethylamino)propyl] methacrylamide) (P1) and poly(N-(3-aminopropyl)methacrylamide) (P2), cationic polymers that bind to the surface of V. cholerae, the infectious agent causing cholera disease, can sequester the pathogen into clusters. Upon clustering, V. cholerae transitions to a sessile lifestyle, characterised by increased biofilm production and the repression of key virulence factors such as the cholera toxin (CTX). Moreover, clustering the pathogen results in the minimisation of adherence and toxicity to intestinal epithelial cells. Our results suggest that the reduction in toxicity is associated with the reduction to the number of free bacteria, but also the downregulation of toxin production. Finally we demonstrate that these polymers can reduce colonisation of zebrafish larvae upon ingestion of water contaminated with V. cholerae. Overall, our results suggest that the physiology of this pathogen can be modulated without the need to genetically manipulate the microorganism and that this modulation is an off-target effect that results from the intrinsic ability of the pathogen to sense and adapt to its environment. We believe these findings pave the way towards a better understanding of the interactions between pathogenic bacteria and polymeric materials and will underpin the development of novel antimicrobial polymers.

AB - Here we report the first application of non-bactericidal synthetic polymers to modulate the physiology of a bacterial pathogen. Poly(N-[3- (dimethylamino)propyl] methacrylamide) (P1) and poly(N-(3-aminopropyl)methacrylamide) (P2), cationic polymers that bind to the surface of V. cholerae, the infectious agent causing cholera disease, can sequester the pathogen into clusters. Upon clustering, V. cholerae transitions to a sessile lifestyle, characterised by increased biofilm production and the repression of key virulence factors such as the cholera toxin (CTX). Moreover, clustering the pathogen results in the minimisation of adherence and toxicity to intestinal epithelial cells. Our results suggest that the reduction in toxicity is associated with the reduction to the number of free bacteria, but also the downregulation of toxin production. Finally we demonstrate that these polymers can reduce colonisation of zebrafish larvae upon ingestion of water contaminated with V. cholerae. Overall, our results suggest that the physiology of this pathogen can be modulated without the need to genetically manipulate the microorganism and that this modulation is an off-target effect that results from the intrinsic ability of the pathogen to sense and adapt to its environment. We believe these findings pave the way towards a better understanding of the interactions between pathogenic bacteria and polymeric materials and will underpin the development of novel antimicrobial polymers.

U2 - 10.1039/C7SC00615B

DO - 10.1039/C7SC00615B

M3 - Article

JO - Chemical Science

JF - Chemical Science

SN - 2041-6520

ER -