Endothelial dysfunction in hypertension in pregnancy: associations between circulating endothelial cells, circulating progenitor cells and plasma von Willebrand factor.

Research output: Contribution to journalArticle

Authors

  • Vellore Karthikeyan
  • Andrew Blann
  • Sabah Baghdadi
  • D Gareth Beevers

Colleges, School and Institutes

Abstract

BACKGROUND: Endothelial damage/dysfunction has been related to hypertension in pregnancy, with implications in pregnancy outcomes. We hypothesised abnormal levels of circulating endothelial cells (CECs), circulating progenitor cells (CPCs) and plasma von Willebrand factor (vWf, a marker of endothelial damage/dysfunction) in pregnant women with hypertension, when compared to pregnant normotensives and non pregnant healthy controls. METHODS: Our study groups were 3rd trimester hypertensive pregnant women, 40 age matched normotensive pregnant women and 50 non pregnant healthy controls. CECs were measured by immunomagnetic separation using anti-CD146 monoclonal antibody coated beads. CPCs were defined using flow cytometry as CD133+/CD34+/CD45-. vWf was measured by ELISA. RESULTS: Hypertensive pregnant women had significantly higher CECs compared to normotensive pregnant women and non pregnant healthy controls (p 

Details

Original languageEnglish
Pages (from-to)531-537
Number of pages7
JournalClinical Research in Cardiology
Volume100
Publication statusPublished - 9 Jan 2011