Endothelial dysfunction in hypertension in pregnancy: associations between circulating endothelial cells, circulating progenitor cells and plasma von Willebrand factor.

Vellore Karthikeyan; Andrew Blann; Sabah Baghdadi; Deirdre Lane; D Gareth Beevers; Gregory Lip

doi:10.1007/s00392-010-0277-9

Endothelial dysfunction in hypertension in pregnancy: associations between circulating endothelial cells, circulating progenitor cells and plasma von Willebrand factor.

Vellore Karthikeyan, Andrew Blann, Sabah Baghdadi, Deirdre Lane, D Gareth Beevers, Gregory Lip

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Abstract

BACKGROUND: Endothelial damage/dysfunction has been related to hypertension in pregnancy, with implications in pregnancy outcomes. We hypothesised abnormal levels of circulating endothelial cells (CECs), circulating progenitor cells (CPCs) and plasma von Willebrand factor (vWf, a marker of endothelial damage/dysfunction) in pregnant women with hypertension, when compared to pregnant normotensives and non pregnant healthy controls. METHODS: Our study groups were 3rd trimester hypertensive pregnant women, 40 age matched normotensive pregnant women and 50 non pregnant healthy controls. CECs were measured by immunomagnetic separation using anti-CD146 monoclonal antibody coated beads. CPCs were defined using flow cytometry as CD133+/CD34+/CD45-. vWf was measured by ELISA. RESULTS: Hypertensive pregnant women had significantly higher CECs compared to normotensive pregnant women and non pregnant healthy controls (p

Original language	English
Pages (from-to)	531-537
Number of pages	7
Journal	Clinical Research in Cardiology
Volume	100
DOIs	https://doi.org/10.1007/s00392-010-0277-9
Publication status	Published - 9 Jan 2011

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title = "Endothelial dysfunction in hypertension in pregnancy: associations between circulating endothelial cells, circulating progenitor cells and plasma von Willebrand factor.",

abstract = "BACKGROUND: Endothelial damage/dysfunction has been related to hypertension in pregnancy, with implications in pregnancy outcomes. We hypothesised abnormal levels of circulating endothelial cells (CECs), circulating progenitor cells (CPCs) and plasma von Willebrand factor (vWf, a marker of endothelial damage/dysfunction) in pregnant women with hypertension, when compared to pregnant normotensives and non pregnant healthy controls. METHODS: Our study groups were 3rd trimester hypertensive pregnant women, 40 age matched normotensive pregnant women and 50 non pregnant healthy controls. CECs were measured by immunomagnetic separation using anti-CD146 monoclonal antibody coated beads. CPCs were defined using flow cytometry as CD133+/CD34+/CD45-. vWf was measured by ELISA. RESULTS: Hypertensive pregnant women had significantly higher CECs compared to normotensive pregnant women and non pregnant healthy controls (p ",

author = "Vellore Karthikeyan and Andrew Blann and Sabah Baghdadi and Deirdre Lane and {Gareth Beevers}, D and Gregory Lip",

year = "2011",

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doi = "10.1007/s00392-010-0277-9",

language = "English",

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journal = "Clinical Research in Cardiology",

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T1 - Endothelial dysfunction in hypertension in pregnancy: associations between circulating endothelial cells, circulating progenitor cells and plasma von Willebrand factor.

AU - Karthikeyan, Vellore

AU - Blann, Andrew

AU - Baghdadi, Sabah

AU - Lane, Deirdre

AU - Gareth Beevers, D

AU - Lip, Gregory

PY - 2011/1/9

Y1 - 2011/1/9

N2 - BACKGROUND: Endothelial damage/dysfunction has been related to hypertension in pregnancy, with implications in pregnancy outcomes. We hypothesised abnormal levels of circulating endothelial cells (CECs), circulating progenitor cells (CPCs) and plasma von Willebrand factor (vWf, a marker of endothelial damage/dysfunction) in pregnant women with hypertension, when compared to pregnant normotensives and non pregnant healthy controls. METHODS: Our study groups were 3rd trimester hypertensive pregnant women, 40 age matched normotensive pregnant women and 50 non pregnant healthy controls. CECs were measured by immunomagnetic separation using anti-CD146 monoclonal antibody coated beads. CPCs were defined using flow cytometry as CD133+/CD34+/CD45-. vWf was measured by ELISA. RESULTS: Hypertensive pregnant women had significantly higher CECs compared to normotensive pregnant women and non pregnant healthy controls (p

AB - BACKGROUND: Endothelial damage/dysfunction has been related to hypertension in pregnancy, with implications in pregnancy outcomes. We hypothesised abnormal levels of circulating endothelial cells (CECs), circulating progenitor cells (CPCs) and plasma von Willebrand factor (vWf, a marker of endothelial damage/dysfunction) in pregnant women with hypertension, when compared to pregnant normotensives and non pregnant healthy controls. METHODS: Our study groups were 3rd trimester hypertensive pregnant women, 40 age matched normotensive pregnant women and 50 non pregnant healthy controls. CECs were measured by immunomagnetic separation using anti-CD146 monoclonal antibody coated beads. CPCs were defined using flow cytometry as CD133+/CD34+/CD45-. vWf was measured by ELISA. RESULTS: Hypertensive pregnant women had significantly higher CECs compared to normotensive pregnant women and non pregnant healthy controls (p

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DO - 10.1007/s00392-010-0277-9

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JO - Clinical Research in Cardiology

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Endothelial dysfunction in hypertension in pregnancy: associations between circulating endothelial cells, circulating progenitor cells and plasma von Willebrand factor.

Abstract

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