Endosialin (TEM1, CD248) is a marker of stromal fibroblasts and is not selectively expressed on tumour endothelium

JR MacFadyen; OJ Haworth; D Robertson; Deborah Hardie; MT Webster; HR Morris; M Panico; M Sutton-Smith; A Dell; P van der Geer; D Wienke; Christopher Buckley; CM Isacke

doi:10.1016/j.febslet.2005.03.071

Endosialin (TEM1, CD248) is a marker of stromal fibroblasts and is not selectively expressed on tumour endothelium

JR MacFadyen, OJ Haworth, D Robertson, Deborah Hardie, MT Webster, HR Morris, M Panico, M Sutton-Smith, A Dell, P van der Geer, D Wienke, Christopher Buckley, CM Isacke

Immunology and Immunotherapy

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125 Citations (Scopus)

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Abstract

Fibroblasts are a diverse cell type and display clear topographic differentiation and positional memory. In a screen for fibroblast specific markers we have characterized four monoclonal antibodies to endosialin (TEM1/CD248). Previous studies have reported that endosialin is a tumour endothelium marker and is localized intracellularly. We demonstrate conclusively that endosialin is a cell surface glycoprotein and is predominantly expressed by fibroblasts and a subset of pericytes associated with tumour vessels but not by tumour endothelium. These novel antibodies will facilitate the isolation and classification of fibroblast and pericyte lineages as well as the further functional analysis of endosialin.

Original language	English
Pages (from-to)	2569-75
Number of pages	7
Journal	FEBS Letters
Volume	579
Issue number	12
Early online date	7 Apr 2005
DOIs	https://doi.org/10.1016/j.febslet.2005.03.071
Publication status	Published - 9 May 2005

Keywords

TEM
CD248
pericyte
fibroblast
angiogenesis
endosialin

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10.1016/j.febslet.2005.03.071

MacFadyen_et_al_Endosialin_marker_stromal_fibroblasts_FEBS_Letters_2005
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MacFadyen, JR., Haworth, OJ., Robertson, D., Hardie, D., Webster, MT., Morris, HR., Panico, M., Sutton-Smith, M., Dell, A., van der Geer, P., Wienke, D., Buckley, C., & Isacke, CM. (2005). Endosialin (TEM1, CD248) is a marker of stromal fibroblasts and is not selectively expressed on tumour endothelium. FEBS Letters, 579(12), 2569-75. https://doi.org/10.1016/j.febslet.2005.03.071

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abstract = "Fibroblasts are a diverse cell type and display clear topographic differentiation and positional memory. In a screen for fibroblast specific markers we have characterized four monoclonal antibodies to endosialin (TEM1/CD248). Previous studies have reported that endosialin is a tumour endothelium marker and is localized intracellularly. We demonstrate conclusively that endosialin is a cell surface glycoprotein and is predominantly expressed by fibroblasts and a subset of pericytes associated with tumour vessels but not by tumour endothelium. These novel antibodies will facilitate the isolation and classification of fibroblast and pericyte lineages as well as the further functional analysis of endosialin.",

keywords = "TEM, CD248, pericyte, fibroblast, angiogenesis, endosialin",

author = "JR MacFadyen and OJ Haworth and D Robertson and Deborah Hardie and MT Webster and HR Morris and M Panico and M Sutton-Smith and A Dell and {van der Geer}, P and D Wienke and Christopher Buckley and CM Isacke",

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MacFadyen, JR, Haworth, OJ, Robertson, D, Hardie, D, Webster, MT, Morris, HR, Panico, M, Sutton-Smith, M, Dell, A, van der Geer, P, Wienke, D, Buckley, C & Isacke, CM 2005, 'Endosialin (TEM1, CD248) is a marker of stromal fibroblasts and is not selectively expressed on tumour endothelium', FEBS Letters, vol. 579, no. 12, pp. 2569-75. https://doi.org/10.1016/j.febslet.2005.03.071

TY - JOUR

T1 - Endosialin (TEM1, CD248) is a marker of stromal fibroblasts and is not selectively expressed on tumour endothelium

AU - MacFadyen, JR

AU - Haworth, OJ

AU - Robertson, D

AU - Hardie, Deborah

AU - Webster, MT

AU - Morris, HR

AU - Panico, M

AU - Sutton-Smith, M

AU - Dell, A

AU - van der Geer, P

AU - Wienke, D

AU - Buckley, Christopher

AU - Isacke, CM

PY - 2005/5/9

Y1 - 2005/5/9

N2 - Fibroblasts are a diverse cell type and display clear topographic differentiation and positional memory. In a screen for fibroblast specific markers we have characterized four monoclonal antibodies to endosialin (TEM1/CD248). Previous studies have reported that endosialin is a tumour endothelium marker and is localized intracellularly. We demonstrate conclusively that endosialin is a cell surface glycoprotein and is predominantly expressed by fibroblasts and a subset of pericytes associated with tumour vessels but not by tumour endothelium. These novel antibodies will facilitate the isolation and classification of fibroblast and pericyte lineages as well as the further functional analysis of endosialin.

AB - Fibroblasts are a diverse cell type and display clear topographic differentiation and positional memory. In a screen for fibroblast specific markers we have characterized four monoclonal antibodies to endosialin (TEM1/CD248). Previous studies have reported that endosialin is a tumour endothelium marker and is localized intracellularly. We demonstrate conclusively that endosialin is a cell surface glycoprotein and is predominantly expressed by fibroblasts and a subset of pericytes associated with tumour vessels but not by tumour endothelium. These novel antibodies will facilitate the isolation and classification of fibroblast and pericyte lineages as well as the further functional analysis of endosialin.

KW - TEM

KW - CD248

KW - pericyte

KW - fibroblast

KW - angiogenesis

KW - endosialin

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U2 - 10.1016/j.febslet.2005.03.071

DO - 10.1016/j.febslet.2005.03.071

M3 - Article

C2 - 15862292

SN - 1873-3468

VL - 579

SP - 2569

EP - 2575

JO - FEBS Letters

JF - FEBS Letters

IS - 12

ER -

Endosialin (TEM1, CD248) is a marker of stromal fibroblasts and is not selectively expressed on tumour endothelium

Abstract

Keywords

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