Abstract
The effect of cytochalasin B (CB) and cytochalasin D (CD) on the endocytotic uptake of horseradish peroxidase (HRP) by intestinal absorptive cells was investigated by morphometric methods. The results showed that CD inhibited endocytosis considerably, and without any detrimental side-effects. CB had hardly any effect on the endocytosis of HRP, but caused a significant decrease in the number of apical vesicles and tubules involved in the transport of cell-coat glycoproteins from the Golgi apparatus to the brush border. Electron-microscopic autoradiographic analysis of the effect of CD showed that although endocytosis is inhibited significantly by the drug, the amount of radiolabelled cell-coat material entering the lysosome-like bodies was unaltered compared with control cultures. These observations support our hypothesis that the cell-coat glycoproteins of the absorptive cells enter the lysosome-like bodies by a crinophagic rather than by an exocytotic-endocytotic mechanism.
Original language | English |
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Pages (from-to) | 113-26 |
Number of pages | 14 |
Journal | Cell and tissue research |
Volume | 222 |
Issue number | 1 |
Publication status | Published - 1982 |
Keywords
- Endocytosis
- Dimethyl Sulfoxide
- Cells, Cultured
- Humans
- Horseradish Peroxidase
- Microscopy, Electron
- Intestinal Absorption
- Glycoproteins
- Cytochalasin B
- Cytochalasins