Electrical stimulation changes human mesenchymal stem cells orientation and cytoskeleton organization

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Electrical stimulation changes human mesenchymal stem cells orientation and cytoskeleton organization. / Mobini, Sahba; Talts, Ülle Linda; Xue, Ruikang; Cassidy, Nigel J.; Cartmell, Sarah H.

In: Journal of Biomaterials and Tissue Engineering, Vol. 7, No. 9, 16-832-R, 01.09.2017, p. 829-833.

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Mobini, Sahba ; Talts, Ülle Linda ; Xue, Ruikang ; Cassidy, Nigel J. ; Cartmell, Sarah H. / Electrical stimulation changes human mesenchymal stem cells orientation and cytoskeleton organization. In: Journal of Biomaterials and Tissue Engineering. 2017 ; Vol. 7, No. 9. pp. 829-833.

Bibtex

@article{98f4bff76c9f430299537c8043a27f90,
title = "Electrical stimulation changes human mesenchymal stem cells orientation and cytoskeleton organization",
abstract = "Electricity, as a physical stimulus, is recently becoming an attractive tool fortissue engineering. In this study we simulated the electrical field delivered by asimplified electro-bioreactor, using finite element analysis. In addition, humanmesenchymal stem cells (hMSCs) were cultured in an electro-bioreactor andan electrical field of 100 mV/mm for 1 hour per day was applied. The cell profile,orientation and cytoskeleton changes by CellProfiler was analysed at 1, 2, 3and 7 days. The cytoskeleton texture of cells exposed to electrical stimulationwas also compared with cells exposed to chemical stimulation during an earlyphase of osteogenic differentiation. Results showed that hMSCs orientationand cytoskeleton actin filaments reorganize perpendicular to the electrical fieldin the vicinity of the cathode area at day 7. This finding and analysis methodhas the potential to provide a framework for future studies of mechanismunderlying the changes in cell profile in electrical fields.",
keywords = "Electrical stimulation, Cell orientation, Computational modelling, Cytoskeleton reorganization",
author = "Sahba Mobini and Talts, {{\"U}lle Linda} and Ruikang Xue and Cassidy, {Nigel J.} and Cartmell, {Sarah H.}",
year = "2017",
month = sep
day = "1",
doi = "10.1166/jbt.2017.1631",
language = "English",
volume = "7",
pages = "829--833",
journal = "Journal of Biomaterials and Tissue Engineering",
issn = "2157-9083",
publisher = "American Scientific Publishers",
number = "9",

}

RIS

TY - JOUR

T1 - Electrical stimulation changes human mesenchymal stem cells orientation and cytoskeleton organization

AU - Mobini, Sahba

AU - Talts, Ülle Linda

AU - Xue, Ruikang

AU - Cassidy, Nigel J.

AU - Cartmell, Sarah H.

PY - 2017/9/1

Y1 - 2017/9/1

N2 - Electricity, as a physical stimulus, is recently becoming an attractive tool fortissue engineering. In this study we simulated the electrical field delivered by asimplified electro-bioreactor, using finite element analysis. In addition, humanmesenchymal stem cells (hMSCs) were cultured in an electro-bioreactor andan electrical field of 100 mV/mm for 1 hour per day was applied. The cell profile,orientation and cytoskeleton changes by CellProfiler was analysed at 1, 2, 3and 7 days. The cytoskeleton texture of cells exposed to electrical stimulationwas also compared with cells exposed to chemical stimulation during an earlyphase of osteogenic differentiation. Results showed that hMSCs orientationand cytoskeleton actin filaments reorganize perpendicular to the electrical fieldin the vicinity of the cathode area at day 7. This finding and analysis methodhas the potential to provide a framework for future studies of mechanismunderlying the changes in cell profile in electrical fields.

AB - Electricity, as a physical stimulus, is recently becoming an attractive tool fortissue engineering. In this study we simulated the electrical field delivered by asimplified electro-bioreactor, using finite element analysis. In addition, humanmesenchymal stem cells (hMSCs) were cultured in an electro-bioreactor andan electrical field of 100 mV/mm for 1 hour per day was applied. The cell profile,orientation and cytoskeleton changes by CellProfiler was analysed at 1, 2, 3and 7 days. The cytoskeleton texture of cells exposed to electrical stimulationwas also compared with cells exposed to chemical stimulation during an earlyphase of osteogenic differentiation. Results showed that hMSCs orientationand cytoskeleton actin filaments reorganize perpendicular to the electrical fieldin the vicinity of the cathode area at day 7. This finding and analysis methodhas the potential to provide a framework for future studies of mechanismunderlying the changes in cell profile in electrical fields.

KW - Electrical stimulation

KW - Cell orientation

KW - Computational modelling

KW - Cytoskeleton reorganization

UR - http://www.scopus.com/inward/record.url?scp=85029211281&partnerID=8YFLogxK

U2 - 10.1166/jbt.2017.1631

DO - 10.1166/jbt.2017.1631

M3 - Article

AN - SCOPUS:85029211281

VL - 7

SP - 829

EP - 833

JO - Journal of Biomaterials and Tissue Engineering

JF - Journal of Biomaterials and Tissue Engineering

SN - 2157-9083

IS - 9

M1 - 16-832-R

ER -