Efflux in Acinetobacter baumannii can be determined by measuring accumulation of H33342 (bis-benzamide)

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Efflux in Acinetobacter baumannii can be determined by measuring accumulation of H33342 (bis-benzamide). / Richmond, G E; Chua, K L; Piddock, L J V.

In: Journal of Antimicrobial Chemotherapy, Vol. 68, No. 7, 07.2013, p. 1594-600.

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@article{7a814b53a8914f4583ee22abbff1673b,
title = "Efflux in Acinetobacter baumannii can be determined by measuring accumulation of H33342 (bis-benzamide)",
abstract = "Overexpression of efflux pumps in Acinetobacter baumannii is a common mechanism of multidrug resistance in this nosocomial pathogen. Increased efflux pump expression is often assumed from MICs of antibiotics and dyes, without measurement of efflux levels. This study describes a safe, rapid and simple 96-well plate assay that measures the accumulation of a fluorescent dye, Hoechst (H) 33342.",
author = "Richmond, {G E} and Chua, {K L} and Piddock, {L J V}",
year = "2013",
month = jul,
doi = "10.1093/jac/dkt052",
language = "English",
volume = "68",
pages = "1594--600",
journal = "Journal of Antimicrobial Chemotherapy",
issn = "0305-7453",
publisher = "Oxford University Press",
number = "7",

}

RIS

TY - JOUR

T1 - Efflux in Acinetobacter baumannii can be determined by measuring accumulation of H33342 (bis-benzamide)

AU - Richmond, G E

AU - Chua, K L

AU - Piddock, L J V

PY - 2013/7

Y1 - 2013/7

N2 - Overexpression of efflux pumps in Acinetobacter baumannii is a common mechanism of multidrug resistance in this nosocomial pathogen. Increased efflux pump expression is often assumed from MICs of antibiotics and dyes, without measurement of efflux levels. This study describes a safe, rapid and simple 96-well plate assay that measures the accumulation of a fluorescent dye, Hoechst (H) 33342.

AB - Overexpression of efflux pumps in Acinetobacter baumannii is a common mechanism of multidrug resistance in this nosocomial pathogen. Increased efflux pump expression is often assumed from MICs of antibiotics and dyes, without measurement of efflux levels. This study describes a safe, rapid and simple 96-well plate assay that measures the accumulation of a fluorescent dye, Hoechst (H) 33342.

U2 - 10.1093/jac/dkt052

DO - 10.1093/jac/dkt052

M3 - Article

C2 - 23467176

VL - 68

SP - 1594

EP - 1600

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

SN - 0305-7453

IS - 7

ER -