Efflux in Acinetobacter baumannii can be determined by measuring accumulation of H33342 (bis-benzamide)

Research output: Contribution to journalArticlepeer-review


Colleges, School and Institutes


Overexpression of efflux pumps in Acinetobacter baumannii is a common mechanism of multidrug resistance in this nosocomial pathogen. Increased efflux pump expression is often assumed from MICs of antibiotics and dyes, without measurement of efflux levels. This study describes a safe, rapid and simple 96-well plate assay that measures the accumulation of a fluorescent dye, Hoechst (H) 33342.


Original languageEnglish
Pages (from-to)1594-600
Number of pages7
JournalJournal of Antimicrobial Chemotherapy
Issue number7
Publication statusPublished - Jul 2013