Effects of plasticisers and related compounds on the expression of the soluble form of catechol-O-methyltransferase in MCF-7 cells

Research output: Contribution to journalReview article

Authors

  • PWL Ho
  • ACY Chu
  • KHH Kwok
  • HF Liu
  • MHW Kung
  • SL Ho

Colleges, School and Institutes

Abstract

Previously we have shown that E2 down regulates S-COMT expression. Here the effects of four phthalate esters and 4-(tert-octyl) phenol on the intra-cellular levels of S-COMT and COMT activity were studied in MCF-7 cells as a measure of estrogenic activity of these compounds. The four phthalate esters caused significant reductions in both S-COMT protein and COMT activity levels. These effects were inhibited by the ER receptor antagonist ICI182780. 4-(tert-octyl) phenol also caused reductions in these parameters, but the effects were not abolished by ICI182780. Assay of S-COMT protein levels represents a simple and convenient method of assessing the estrogenic potential of a compound.

Details

Original languageEnglish
Pages (from-to)276-279
Number of pages4
JournalCurrent Drug Metabolism
Volume9
Issue number4
Publication statusPublished - 1 May 2008

Keywords

  • plasticiser, estrogen, phthalate, endocrine disruption, COMT