Effects of freezing, fixation and dehydration on surface roughness properties of porcine left anterior descending coronary arteries

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@article{6cb6a7ae55c14fc3af8878e75abd663f,
title = "Effects of freezing, fixation and dehydration on surface roughness properties of porcine left anterior descending coronary arteries",
abstract = "Background: To allow measurements of surface roughness to be made of coronary arteries using various imaging techniques, chemical processing, such as fixation and dehydration, is commonly used. Standard protocols suggest storing fresh biological tissue at -40°C. The aim of this study was to quantify the changes caused by freezing and chemical processing to the surface roughness measurements of coronary arteries, and to determine whether correction factors are needed for surface roughness measurements of coronary arteries following chemical processes typically used before imaging these arteries.Methods: Porcine left anterior descending coronary arteries were dissected ex vivo. Surface roughness was then calculated following three-dimensional reconstruction of surface images obtained using an optical microscope. Surface roughness was measured before and after a freeze cycle to assess changes during freezing, after chemical fixation, and again after dehydration, to determine changes during these steps of chemical processing.Results: No significant difference was caused due to the freeze cycle (p > 0.05). There was no significant difference in the longitudinally measured surface roughness (RaL = 0.99 ± 0.39 µm; p > 0.05) of coronary arteries following fixation and dehydration either. However, the circumferentially measured surface roughness increased significantly following a combined method of processing (RaC = 1.36 ± 0.40, compared 1.98 ± 0.27 µm, respectively; p < 0.05). A correction factor can compensate for the change in RaC due to processing of tissue, 〖Ra〗_Cβ = 〖Ra〗_C/(1+0.46). Where RaCβ, the corrected RaC, had a mean of 1.31 ± 0.21 µm.Conclusions: Independently, freezing, fixation and dehydration do not alter the surface roughness of coronary arteries. Combined, however, fixation and dehydration significantly increase the circumferential, but not longitudinal, surface roughness of coronary arteries.",
keywords = "Coronary arteries, Dehydration, Fixation, Freezing, Surface imaging, Surface roughness",
author = "Hanna Burton and Richard Williams and Daniel Espino",
year = "2017",
month = jun,
day = "22",
doi = "10.1016/j.micron.2017.06.009",
language = "English",
journal = "Micron",
issn = "0968-4328",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Effects of freezing, fixation and dehydration on surface roughness properties of porcine left anterior descending coronary arteries

AU - Burton, Hanna

AU - Williams, Richard

AU - Espino, Daniel

PY - 2017/6/22

Y1 - 2017/6/22

N2 - Background: To allow measurements of surface roughness to be made of coronary arteries using various imaging techniques, chemical processing, such as fixation and dehydration, is commonly used. Standard protocols suggest storing fresh biological tissue at -40°C. The aim of this study was to quantify the changes caused by freezing and chemical processing to the surface roughness measurements of coronary arteries, and to determine whether correction factors are needed for surface roughness measurements of coronary arteries following chemical processes typically used before imaging these arteries.Methods: Porcine left anterior descending coronary arteries were dissected ex vivo. Surface roughness was then calculated following three-dimensional reconstruction of surface images obtained using an optical microscope. Surface roughness was measured before and after a freeze cycle to assess changes during freezing, after chemical fixation, and again after dehydration, to determine changes during these steps of chemical processing.Results: No significant difference was caused due to the freeze cycle (p > 0.05). There was no significant difference in the longitudinally measured surface roughness (RaL = 0.99 ± 0.39 µm; p > 0.05) of coronary arteries following fixation and dehydration either. However, the circumferentially measured surface roughness increased significantly following a combined method of processing (RaC = 1.36 ± 0.40, compared 1.98 ± 0.27 µm, respectively; p < 0.05). A correction factor can compensate for the change in RaC due to processing of tissue, 〖Ra〗_Cβ = 〖Ra〗_C/(1+0.46). Where RaCβ, the corrected RaC, had a mean of 1.31 ± 0.21 µm.Conclusions: Independently, freezing, fixation and dehydration do not alter the surface roughness of coronary arteries. Combined, however, fixation and dehydration significantly increase the circumferential, but not longitudinal, surface roughness of coronary arteries.

AB - Background: To allow measurements of surface roughness to be made of coronary arteries using various imaging techniques, chemical processing, such as fixation and dehydration, is commonly used. Standard protocols suggest storing fresh biological tissue at -40°C. The aim of this study was to quantify the changes caused by freezing and chemical processing to the surface roughness measurements of coronary arteries, and to determine whether correction factors are needed for surface roughness measurements of coronary arteries following chemical processes typically used before imaging these arteries.Methods: Porcine left anterior descending coronary arteries were dissected ex vivo. Surface roughness was then calculated following three-dimensional reconstruction of surface images obtained using an optical microscope. Surface roughness was measured before and after a freeze cycle to assess changes during freezing, after chemical fixation, and again after dehydration, to determine changes during these steps of chemical processing.Results: No significant difference was caused due to the freeze cycle (p > 0.05). There was no significant difference in the longitudinally measured surface roughness (RaL = 0.99 ± 0.39 µm; p > 0.05) of coronary arteries following fixation and dehydration either. However, the circumferentially measured surface roughness increased significantly following a combined method of processing (RaC = 1.36 ± 0.40, compared 1.98 ± 0.27 µm, respectively; p < 0.05). A correction factor can compensate for the change in RaC due to processing of tissue, 〖Ra〗_Cβ = 〖Ra〗_C/(1+0.46). Where RaCβ, the corrected RaC, had a mean of 1.31 ± 0.21 µm.Conclusions: Independently, freezing, fixation and dehydration do not alter the surface roughness of coronary arteries. Combined, however, fixation and dehydration significantly increase the circumferential, but not longitudinal, surface roughness of coronary arteries.

KW - Coronary arteries

KW - Dehydration

KW - Fixation

KW - Freezing

KW - Surface imaging

KW - Surface roughness

U2 - 10.1016/j.micron.2017.06.009

DO - 10.1016/j.micron.2017.06.009

M3 - Article

JO - Micron

JF - Micron

SN - 0968-4328

ER -